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"Aktivitas pengelolaan minyak bumi terus meningkat sehingga diperlukan tindakan pengelolaan pencemaran. Penelitian ini bertujuan untuk mengetahui perbedaan pertumbuhan bakteri dan penurunan kadar TPH pada proses bioremediasi yang distimulasi dengan penambahan kompos dan lumpur IPAL pada 5% dan 10% secara eksperimental skala laboratorium dengan simulasi tanah tercemar dengan kadar TPH sebesar 5,5% selama 5 minggu hingga mencapai baku mutu yaitu di bawah 1%. Tanah yang digunakan berasal dari pantai Marunda, kompos dari UPS Merdeka, lumpur IPAL dari Jababeka, dan bakteri diisolat dari tanah tercemar minyak diwilayah sekitrar kilang. Hasilnya berupa laju pertumbuhan bakteri pada kompos dan lumpur IPAL dengan kadar 5% masing-masing adalah 0,7567/minggu dan 1,154/minggu serta 0,8783/minggu dan 1,1109/minggu pada kadar 10%. Sedangkan efisiensi penyisihan TPH yang didapatkan adalah 95,32% dan 96,85% untuk penambahan kompos 5% dan 10% serta 91,15% dan 91,02% untuk penambahan lumpur IPAL sebanyak 5% dan 10%. Hasil uji t menyatakan perbedaan baik pertumbuhan bakteri maupun penurunan kadar TPH tidak signifikan. Kemudian hasil uji korelasi menunjukkan korelasi rendah berbanding terbalik untuk hubungan TPH dengan jumlah bakteri.

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Crude oil's processing into energy continuous to increase, hence the treatment for its environmental impact is needed. This study aims to determine the differences of bacterial growth rate and removal efficiency of Total Petroleum Hydrocarbon (TPH) between compost and WWTP sludge addtion at 5% and 10%. Those effect was acknowledge through experimental in laboratory scale using soil contaminated by 5,5% TPH within 5 weeks until it reach less than 1% as the requirement. The soil comes from Marunda Beach, compost from UPS Merdeka, WWTP sludge from Jababeka, bacterial isolated from soil contaminated at the surrounding of refining.

Result of this study showed that the bacterial growth rate in compost and WWTP sludge at 5% and 10% concentration each are 0,7567/weeks and 1,154/week for compost also 0,8783/week and 1,1109/week for WWTP sludge. While the TPH removal efficiency obtained was 95,32% and 96,85% for the addition of compsot as well as 91,15% and 91,02% for the addition of WWTP sludge.at 5% and 10% concentration. Due to t-Test, the differences between all the variation of concentration are not significant. The correlation test between TPH degradation to bacterial growth showed that there is a weak downhill (negative) linear relationship."

"ABSTRACTCrude oil's processing into energy continue to increase, hence treatment for its environmental impact is needed. The objectives of the study is to determine the differences in bacteria growth rate and removal efficiency of Total Petroleum Hydrocarbon (TPH) between compost and WWTP sludge addition at 5% and 10% concentration levels. Those effects were acknowledge through experiments in laboratory scale using oil contaminated 5,5% TPH within 5 weeks until it reach less than 1% as the requirement. The soil comes from Marunda Beach, compost from UPS Merdeka, WWTP sludge from Jababeka, and bacteria isolated from soil contaminated in the area surrounding refining. The treatment used in this experiment was landfirming with nutrition addition and the main variable analyzed was TPH and the microorganism population. Result of this study show that bacteria growth rate in compost and WWTP sludge at 5% and 10% concentration each are 0,7567/weeks and 1,154/week for compost and also 0,8783/week and 1,1109/week for WWTP sludge. The TPH removal efficiency obtained was95,32% and 96,85% for the addition of compost as well as 91,15% and 91,02% for the addition of WWTP sludge at 5% and 10% concentrations. Base on a t-Test, the differences between all the variation of concentrations are not significant. The correlation test between TPH degradation to bacteria growth showed that there is a week downward (negative) linear relationship."

"Kegiatan industri pertambangan minyak bumi di Indonesia telah menimbulkan banyak kasus pencemaran limbah berbahaya dan beracun (B3). Kasus tersebut dapat menimbulkan dampak buruk bagi kualitas lingkungan. Pada KepMenLH No. 128 Tahun 2003, disebutkan bahwa pemulihan lahan tercemar oleh minyak bumi dapat dilakukan secara biologis, dengan menggunakan kapasitas kemampuan mikroorganisme. Salah satu teknik penerapan pemulihan tersebut adalah dengan menggunakan teknik Bioventing. Tujuan penelitian ini adalah untuk mengetahui pengaruh injeksi udara dan mikroorganisme yang berperan dalam proses remediasi dan faktor-faktor yang mempengaruhi kinerjanya bioventing. Minyak bumi yang digunakan merupakan crude oil yang berasal dari PPPTMGB Lemigas. Selama 5 minggu penelitian, didapatkan penyisihan konsentrasi TPH terbesar yaitu sebesar 82% yang terdapat pada sampel dengan konsentrasi bakteri Bacillus Subtilis 10% v/v. Sedangkan pada sampel dengan konsentrasi bakteri Bacillus Subtilis 15% v/v, dan tanpa penambahan bakteri (bakteri indigenous) 1 dan 2 secara berurut adalah 67,1%, 54,24%, dan 68,12%. Penyisihan konsentrasi BTEX terbesar, yaitu sebesar 66,65% pada kontrol 2. Sedangkan sampel dengan kontrol 1, konsentrasi bakteri Bacillus Subtilis 10% v/v, dan bakteri Bacillus Subtilis 15% v/v secara berurut adalah 23,39%, 34,41%, dan 37,69%. Dari penelitian ini dapat disimpulkan bahwa sampel dengan konsentrasi bakteri Bacillus Subtilis 10% v/v dan Kontrol 2 yang paling baik dalam mendukung efektivitas proses degradasi minyak bumi.

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Oil mining industry in Indonesia has generated many cases of very hazardous waste pollution. Those cases could adversely affect the quality of environment. Ministry of Environment through the Ministry of Environment Decree No. 128/2003, stated that the recovery of oil contaminated area can be purified by using microbial activity, called bioremediation. On of the most preferred methods for the remediation process of oil contaminated soil is bioventing.

The main objective of this study was to determine the effect of air injection and microorganisms that play a role in the remediation process and the factors that affect performance bioventing. Oil used in this study was crude oil which was derived from PPTMGB Lemigas. The purpose of this study. During the 5 weeks of the study, obtained the largest TPH concentrations allowance that is equal to 82% were found in the sample with the concentration of the bacteria Bacillus Subtilis 10% v/v. While the sample with the concentration of bacteria Bacillus Subtilis 15% v/v, and without the addition of bacteria (indigenous) 1 and 2 in sequence is 67.1%, 54.24%, and 68.12%. Provision largest concentration of BTEX, amounting to 66.65% in the control 2. Whereas the control 1, the concentration of the bacteria Bacillus Subtilis 10% v / v, and the bacteria Bacillus Subtilis 15% v / v in the order are 23.39%, 34.41%, and 37.69%.

From this study it can be concluded that the sample with the concentration of the bacteria Bacillus Subtilis 10% v / v and Control 2 is best in support of the effectiveness of oil degradation process."

"Konsorsium bakteri lokal (gabungan Salipiger bermudensis DQ 178660, Alterierythrobacter evoxidivorans DQ 304436, Alteromonas macleodii Y 18228 dan Vibrio harveyi DQ 146936) pendegradasi senyawa hidrokarbon kontaminan yang diisolasi dari kawasan eksplorasi minyak Cepu Jawa Tengah diuji kemampuannya dalam merombak senyawa hidrokarbon minyak bumi yang mencemari tanah di kawasan industri Krakatau Steel Cilegon. Dalam penelitian ini, karakterisasi produksi biosurfaktan yang dihasilkan konsorsium bakteri dilakukan dengan mengevaluasi pola pertumbuhan, analisis tegangan permukaan, analisis tegangan antarmuka, analisis komposisi kimia dan uji aktivitas emulsifikasi. Pengujian selama 30 hari pengamatan meliputi pH, suhu, tekstur tanah empat fraksi (berpasir, liat kasar, liat halus, berdebu), karbon organik, nitrogen organik, rasio karbon/nitrogen organik, fosfor dan kalium serta analisis sampel tanah tercemar hidrokarbon menggunakan Gas Chromatography-Mass Sphectroscopy (GC-MS). Hasil penelitian menunjukkan bahwa biosurfaktan yang dihasilkan konsorsium bakteri memiliki kemampuan menurunkan tegangan permukaan air lebih tinggi dibanding dengan bakteri tunggal (51 dynes/cm dari 72 dyns/cm), reduksi nilai tegangan antarmuka air dengan minyak paling tinggi dihasilkan konsorsium bakteri (10 dynes/cm), nilai indeks emulsifikasi (93,75%) paling tinggi dihasilkan oleh konsorsium bakteri. Analisis komposisi kimia biosurfaktan yang dihasilkan konsorsium bakteri menunjukkan bahwa biosurfaktan merupakan senyawa kompleks terdiri dari karbohidrat, protein dan lipid. Setelah 30 hari massa inkubasi, hasil analisis GC-MS menunjukkan bahwa bakteri dan konsorsium bakteri mampu merombak senyawa hidrokarbon tersisa yang mencemari tanah di kawasan PT Krakatau Steel Cilegon Banten.;

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Local bacterial consortium (combined of Salipiger bermudensis DQ 178 660, Alterierythrobacter evoxidivorans DQ 304 436, Alteromonas macleodii Y 146 936 and Vibrio harveyi DQ 18228) hydrocarbons degrading contaminants that isolated from oil exploration areas in Cepu Central Java was analyzed for its ability to degrade petroleum hydrocarbons that polluted the soil in industrial area of PT. Krakatau Steel Cilegon.

In this study, characterization of biosurfactant produced by bacterial consortium conducted to evaluate growth patterns, analysis of surface tension, interfacial tension, chemical composition and emulsification activity assay. Analysis for 30 days of observation include pH, temperature, soil texture four fractions (sandy, dusty, rough clayey, smooth clayey), organic carbon, organic nitrogen, the ratio of carbon/nitrogen organic, phosphorus and potassium as well as analysis of hydrocarbon contaminated soil samples using Gas Chromatography -Mass Sphectroscopy (GC-MS).

The results showed that the biosurfactants produced by bacterial consortium have the ability to lower the surface tension of water is higher than with a single bacterium (51 dynes/cm from 72 dyns/cm), the reduction of the highest values ​​of water interfacial tension with oil produced by bacterial consortium (10 dynes/cm ), the highest value of emulsification index (93.75%) produced by bacterial consortium. Analysis of the chemical composition of biosurfactants produced by bacterial consortium showed that biosurfactants are complex compounds composed of carbohydrates, proteins and lipids. After 30 days of incubation time, the results of GC-MS analysis showed that bacteria and bacterial consortium are capable of overhauling the remaining hydrocarbon compounds that polluted the soil in the area of PT Krakatau Steel Cilegon Banten."

"Lumpur padat merupakan endapan suspensi limbah cair dan mikroorganisme yang berasal dari pengolahan air limbah pada IPLT. Pembuatan pupuk kompos merupakan salah satu alternatif yang dapat dilakukan sebagai upaya dalam pemanfaatan lumpur padat karena kandungan yang dimiliki oleh lumpur padat tersebut. Penelitian ini bertujuan untuk menganalisis unsur hara makro dan mikro serta logam berat dan menganalisis potensi aplikasi lumpur padat hasil pengolahan IPLT Pulo gebang serta menganalisis risiko pencemaran logam berat terhadap tanah, tanaman, dan manusia. Pembuatan kompos menggunakan bahan baku berupa lumpur padat hasil pengolahan IPLT Pulo Gebang dan bahan pendukung kompos berupa sampah organik dan dedaunan kering. Metode pengomposan yang digunakan adaah metode open bin composting dengan wadah kompos berupa compost bag 200 L yang dimodifikasi. Pengomposan dilakukan dengan 3 variasi kompos selama 50 hari dimana dilakukan pengamatan suhu, pH, dan kelembapan setiap 2 hari sekali serta pengamatan warna dan bau setiap 1 minggu sekali. Seluruh reaktor kompos berhasil melewati proses pengomposan dengan baik, yaitu berhasil mencapai fase mesofilik, termofilik, dan pendinginan. Berdasarkan hasil uji laboratorium, kompos II dengan perbandingan lumpur padat : sampah organik : dedaunan kering sebesar 50 : 25 : 25 mempunyai kandungan yang paling baik dengan kandungan C-organik sebesar 36,75%, N-Total sebesar 3,57%, rasio C/N sebesar 10,29, P2O5 Total sebesar 2,45%, K2O total sebesar 0,38%, Fe total sebesar 1,97%, Mn sebesar 0,03%, Cu sebesar 33,6 ppm, dan As sebesar 13,09 ppm. Proses pengomposan dapat menaikkan kadar unsur hara makro dan mikro serta menurunkan kadar logam berat. Analisis risiko paparan logam berat terhadap manusia dilakukan dengan 2 metode, yaitu berdasarkan nilai HQ (Health Quotient) dan ECR (Excess Cancer Risk). Kandungan logam berat pada kompos matang mempunyai potensi untuk menimbulkan efek kesehatan dan efek kanker pada 23 – 106 orang dari 10.000 orang.

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Sludge is a liquid waste suspension and microorganisms precipitate from wastewater treatment at WWTP. Compost is an alternative that can be done as an effort to utilize sludge because of the content possessed by the sludge. This study aims to analyze macro and micronutrients as well as heavy metals and analyze the potential application of solid sludge from Pulo Gebang WWTP processing. In addition, also analyze the risk of heavy metal contamination to soil, plants, and humans. Compost production uses raw materials in the from of sludge from Pulo Gebang WWTP processing and compost supporting materials in the form of organic waste and dry leaves. The composting method used is the open bin composting method with a modified 200 L compost bag. Composting was carried out with 3 variations of compost for 50 days where temperature, pH and humidity were observed every 2 days and color and odor observations were made once every 1 week. All of the compost reactors successfully passed the composting process well, successfully achieving the mesophilic, thermophilic, and cooling phases. Based on laboratory test results, compost II with a ratio 50 : 25 : 25 of sludge: organic waste: dry leaves has the best content with C-organic content of 36.75%, N-Total of 3.57%, ratio of C /N 10.29, P2O5 Total 2.45%, K2O total 0.38%, Fe total 1.97%, Mn 0.03%, Cu 33.6 ppm, and As 13, 09 ppm. The composting process can increase levels of macro and micronutrients and reduce levels of heavy metals. Analysis of the risk of heavy metal exposure to humans was carried out using 2 methods, namely based on HQ (Health Quotient) and ECR (Excess Cancer Risk) values. The content of heavy metals in mature compost has the potential to cause health effects and cancer effects in 23-106 people out of 10,000 people."

"ABSTRAKKegiatan eksplorasi dan produksi minyak bumi berpotensi menimbulkan kontaminasi minyak pada tanah, misalnya dari kegiatan operasional, kebocoran pipa, maupun akumulasi timbulan limbah minyak di masa lalu. Keputusan Menteri Lingkungan Hidup no 128 tahun 2003 menyatakan bahwa tanah yang terkontaminasi minyak dikategorikan sebagai limbah bahan berbahaya dan beracun (B3) yang berpotensi menimbulkan kerusakan lingkungan. Pemulihan tanah tercemar oleh minyak bumi dapat dilakukan secara biologis, dengan menggunakan kapasitas kemampuan mikroorganisme. TPH atau Total Petroleum Hydrocarbons dalam hal ini merupakan jumlah hidrokarbon minyak bumi yang terukur dari media tanah. Dalam Keputusan Menteri Lingkungan Hidup No.128 tahun 2003 dijelaskan bahwa target konsentrasi TPH yang aman bagi lingkungan ialah dibawah 1%. Landfarming merupakan salah satu teknik yang dapat diterapkan dalam pemulihan tanah tercemar minyak bumi. Tujuan penelitian ini adalah untuk mengetahui jenis bulking agent yang paling efektif dalam pelaksanaan proses bioremediasi. Sampel tanah terkontaminasi minyak bumi yang diolah diambil dari tanah terkontaminasi minyak mentah (Crude Oil Contaminated Soil) di Pre-treatment pit dalam (SBF) Soil Bioremediation Facility PT. Chevron Pacific Indonesia di Minas, Riau. Bulking agent yang digunakan dalam penelitian adalah serbuk kayu dan cacahan serabut tandan kosong kelapa sawit. Selama 6 minggu penelitian, penyisihan konsentrasi TPH terbesar yaitu 41,04% pada sampel dengan penambahan bulking agent serbuk kayu 4% (w/w). Sedangkan pada sampel dengan penambahan bulking agent serabut tandan kosong kelapa sawit 4% (w/w) dan tanpa penambahan bulking agent berturut-turut adalah 40,45% dan 35,04%. Dari penelitian ini dapat disimpulkan bahwa sampel dengan penambahan bulking agent serbuk kayu 4% (w/w) yang paling efektif dalam proses degradasi minyak bumi.

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ABSTRACTExploration and production of oil has potential to contaminate soil, such as from operations, pipeline leak, and accumulation of oil waste. Ministry of Environment through the Ministry of Environment Decree No. 128/2003, stated that oil contaminated soil is classified as hazardous and toxic waste (B3) that could potentially cause damage to the environment. The remediation of oil contaminated soil can be purified by using microbial activity. TPH or Total Petroleum Hydrocarbons is the amount of petroleum hydrocarbons measured from the soil media. In MOE Decree No. 128/2003 stated that TPH concentrations target that are safe for the environment is below 1%. Landfarming is one of the most preferred technique that can be applied in the remediation of oil contaminated soil. The main purpose of this study was to determine which type of bulking agent is the most effective on bioremediation process. Crude Oil Contaminated Soil sample were taken from pre-treatment pit in Soil Bioremediation Facility PT. Chevron Pacific Indonesia in Minas, Riau. Bulking agents used in the study were wood chip and oil palm shell fiber. During 6 weeks of the study, the largest TPH removal was 41.04% which is a sample with the addition of 4% wood chip (w/w). While the sample with the addition of 4% oil palm shell fiber (w/w) and the sample without the addition of bulking agent were respectively 40.45% and 35.04%. From this study, it can be concluded that the sample with the addition of 4% wood chip (w/w) was the most effective in the crude oil biodegradation process."

"Keterbatasan lahan TPA Cipayung diiringi meningkatnya jumlah timbulan sampah yang dihasilkan memicu pemerintah kota Depok untuk menemukan solusi dalam permasalahan persampahan yaitu dengan mengurangi volume sampah yang masuk ke dalam TPA salah satunya dengan mengolah sampah organik dengan cara pengomposan. Berbagai upaya dilakukan untuk mempercepat proses pengomposan, salah satunya dengan penambahan bulking agent (BA). Tujuan dari penelitian ini yaitu menganalisis karakteristik feedstock, menganalisis pengaruh penambahan bulking agent terhadap pengomposan, menganalisis karakteristik produk kompos dan menganalisis pengaruh penambahan BA terhadap dinamika populasi mikroba. Penelitian ini dilakukan di UPS Merdeka 2 dengan membuat dua gundukan kompos open windrow (tanpa penambahan dan dengan penambahan BA) berukuran 1 m x 1 m x 1,2 m (p x l x t) dengan periode pengomposan selama 91 hari. Penambahan BA berupa cacahan kayu sebesar 25% dari total massa gundukan kompos. Hasil penelitian menunjukkan bahwa BA mampu mempertahankan porositas selama pengomposan sehingga aliran oksigen dapat terdistribusi dengan baik dan dapat menjadi sumber karbon yang baik. Produk akhir kompos G2 memiliki rasio C/N yang lebih tinggi yaitu sebesar 9,04 sedangkan rasio C/N kompos G1 sebesar 5,34. Hasil uji perkecambahan menggunakan tanaman kangkung dan pokchoi menunjukkan nilai GI (%) lebih dari 100% untuk kompos G2 dan nilai GI kompos G1 pada tanaman kangkung sedangkan nilai GI kompos G1 pada tanaman pokchoi sebesar 98,75%. Populasi mikroba pada kompos G2 memiliki populasi yang cenderung lebih tinggi jika dibandingkan dengan kompos G1 dapat disebabkan oleh adanya penambahan porositas.

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The limitation of Cipayung landfill area accompanied by the amount of waste generated has triggered the Depok City government to find solutions to waste problems by reducing the volume of waste entering the landfill, one of which is composting. Various efforts are made to increase the composting process time, one of which is by the addition of bulking agent (BA). The purpose of this study was to analyze the characteristics of organic raw materials, the effect of bulking agents on composting, the characteristics of final product of composting and the effect of adding BA on dynamics of microbial population. This research was conducted in UPS Merdeka 2 by making two open windrow compost mounds (without and with the addition of BA) with each dimension was 1 m x 1 m x 1.2 m (l x w x h) in 91 days period of composting. The addition of BA (wood chips) is 25% of the total mass of the compost mound.

The results showed that BA was able to maintain porosity during composting so the flow of oxygen could be well distributed and could be a good source of carbon. The final product of compost G2 has higher C/N ratio of 9,04 while the C/N ratio of compost G1 was 5,34. The germination test results using water spinach and pokchoi seeds showed GI values (%) more than 100% for compost G2 and GI value of compost G1 using water spinach seeds while GI value of compost G1 using pokchoi seeds was 98,75%. The microbial population of compost G2 has higher population when compared to compost G1s microbial population which can be caused by the addition of porosity in the compost mound."

"Perubahan fisika dan kimia dalam timbunan kompos diteliti selama proses pengomposan limbah pembuatan botol yang terkontaminasi oleh gemuk (grease). Temperature awal menunjukkan peningkatan diikutioleh penurunan secara bertahap pada akhir proses pengomposan...."