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"Polimorfisme genetik IL-1β pada wanita menopause sebagai faktor risiko penyakit periodontal. Penyakit periodontal merupakan penyakit oral dengan etiologi yang multifaktorial serta dapat terjadi pada wanita postmenopause. Tujuan: Penelitian ini bertujuan untuk mengetahui gambaran polimorfisme genetik IL-1β pada populasi wanita postmenopause Indonesia sebagai faktor risiko penyakit periodontal. Metode: Penelitian ini merupakan studi deskriptif menggunakan sampel biologi tersimpan sebanyak 55 buah. Hasil ekstraksi DNA, dilakukan pemeriksaan polimorfisme IL-1β +3954 menggunakan metode PCR-RFLP dengan digest enzim restriksi Taqα 1 kemudian hasilnya dielektroforesis. Hasil penelitian dianalisis menggunakan tes chi-square. Hasil: Gambaran frekuensi genotipe adalah CC 85,7%, CT 14,3%, dan TT tidak ada pada orang yang normal. CC 91,7%, CT 8,3%, and TT tidak ada pada orang yang memiliki penyakit periodontal. Simpulan: Polimorfisme genetik IL-1β pada wanita pascamenopause Indonesia bukan merupakan faktor risiko penyakit periodontal.

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Periodontal disease is a disease with multifactorial etiology that can occur in postmenopausal women. Objective: This research aims to know the description of the genetic polymorphism of IL-1β inpostmenopausal women in Indonesia as a risk factor for periodontal disease. Methods: This study is a descriptive study on 55 biological stored amples. Sample’s extracted DNA was analysed for polymorphisms IL-1β+3954 using PCR-RFLP method with Taqα 1 restriction enzyme digests then the result was electroforized.Research results were analyzed using chi square test. Results: The genotype CC frequency was 85.7%, CT 14.3%, and TT was absent in normal people. CC 86.5%, CT 8.3%, and TT did not exist in people who have periodontal disease. Conclusion: Genetic polymorphisms IL-1β in Indonesia’s postmenopausal women is not a risk factor for periodontal disease. "

"Periodontal status is a periodontum condition evaluated by using plaque index, calculus index, gingival index and pocket index. The main mediator of periodontum inflammation is IL-113 examined by ELISA method. There is an evaluation of PMN s in periodontum inflammation, but the leucotoxin as well as the protease in turn lowers the PMN phagocytotic function. Phagocytotic function was measured by flowcytometry. The aim of the study was to evaluate the high risk factors of being type 2 DM. A diagnostic study was conducted by using cross-sectional design on 45 controlled DM (CDM) subjects, 45 uncontrolled DM (UCDM) subjects in the Metabolic Endocrinology Clinic Cipto Mangunkusumo Hospital Jakarta, as compared to 45 non-DM control subjects. The result of multivariate analysis showed that patients of older age (>54 years old), low periodontum status (periodontal index >1.80), high IL-113 level (>23.70 pg/mL), and low PMN phagocytotic function <<53.47%), were significantly at high risk of having DM compared to non-DM (p<0.05). Lower periodontum status showed an increase in IL-113 level, decrease PMN phagocytotic function, and consequently, an increase in the risk of being type 2 DM."

"Tujuan: Mengetahui hubungan polimorfisme genetik MnSOD Ala-9Val dengan retinoblastoma pada pasien-pasien di Indonesia, serta menilai hubungan polimorfisme gen MnSOD ini dengan aktivitas enzim SOD.Disain: Penelitian kasus-kontrolMetode: Polimorfisme gen MnSOD Ala-9Va1 dideteksi pada 35 pasien retinoblastoma yang berasal dari Divisi Pediatri Departemen Mata RS Cipto Mangunkusumo Jakarta dan 81 kontrol anak sehat dengan menggunakan metode polymerase chain reaction (PCR) dan restriction fragment length polymorphism (RFLP) menggunakan enzim restriksi NgoMIV. Aktivitas SOD dinilai dengan menggunakan prinsip perubahan dl-epinefrin menjadi adenokrom yang dapat dibaca dengan spektrofotometer.Hasil: Pada penelitian ini hanya ditemukan genotip Val/Val dan Ala/Val. Frekuensi polimorfisme gen MnSOD genotip Ala/Val meningkat pada kelompok kasus dibandingkan kontrol meskipun tidak bermakna (OR 2,643 95% CI=0,850-8,217). Frekuensi ale! juga meningkat pada kelompok pasien dibandingkan kontrol (OR=2,46, 95% CI=0,829-7,302). Aktivitas SOD lebih tinggi pada kelompok kasus daripada kontrol (p=0,433). Namun tidak ditemukan perbedaan aktivitas SOD antara kelompok genotip Val/Val dan Ala/Val.Kesimpulan: Sejauh ini frekuensi polimorfisme gen MnSOD Ala-9 Val genotip Ala/Val meningkat pada pasien retinoblastoma, namun genotip ini belum dapat dikatakan sebagai faktor resiko retinoblastoma. Selain itu tidak ditemukan hubungan bermakna antara polimorfisme gen MnSOD Ala-9 Val dengan retinoblastoma dan aktivitas SOD.

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Objectives: In the present study, we investigated the genetic association between a functional polymorphism Ala-9Va1 in the human manganese SOD (MnSOD) gene and retinoblastoma; and the association between this polymorphism and SOD activity.

Methods: This case-control study was examined in 35 retinoblastoma cases and 81 controls. The Ala-9Val polymorphism was detected by PCR and RFLP using NgoMV restriction enzyme. SOD activities was evaluated by the changes of dlepinefrin to adenochrom which measured by spectrofotometry.

Results: No significant differences in the allelic or genotipic distribution between retinoblastoma and controls were observed. Retinoblastoma risk was slightly elevated in Ala/Val genotype (OR: 2,643, 95%CI: 0,85-8,217) as compared with Va JVal genotype. We did not find AlalAla genotype in both groups. There was significant difference in SOD activity between cases and controls (p=0,033). The SOD activity was higher in retinoblastoma than controls.

Conclusions: The MnSOD gene polymorphism Ala-9Val was not found to be associated with retinoblastoma in this case-control study. It seemed that the Ala-9Val polymorphism was not a risk factor for retinoblastoma. There was also no association between MnSOD gene Ala-9VaI polymorphism and SOD activities. Studies with a larger sample size are needed to confirm the findings."

"Latar belakang: Periodontitis adalah penyakit kronis terlokalisasi pada jaringan penyangga gigi. Penyebab penyakit ini multifaktorial, termasuk faktor genetik.Tujuan: Menganalisis hubungan polimorfisme genetik IL-10 pada laki-laki terhadap derajat keparahan periodontitis.Metode: Menggunakan tehnik PCR dan RFLP (enzim RSA I), dianalisis dengan elektroforesis dan divisualisasi menggunakan Gel Doc.Hasil: 44 sampel normal terdapat genotip: CC 27,27%, CA 41,37%, AA 11,36%; 70 kelompok periodontitis : ringan CC 50%, CA 12,5%, AA 37,5%; sedang: CC 38,9%, CA 47,2%, AA 13,9%; berat: CC 42,3%, CA 30,8%, AA 19,4%.Kesimpulan: Ditemukan gambaran polimorfisme IL-10 pada pada penelitian ini, namun tidak berhubungan dengan derajat keparahan periodontitis.(p>0.05).

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Background: Periodontitis is a chronic disease localized to the supporting tissue and bone of teeth. It is multifactorial, including genetic factors.

Aim: To analyze the relationship of genetic polymorphisms in the IL-10 men against the severity of periodontitis.

Methods: Using PCR and RFLP techniques (RSA enzyme I), were analyzed by electrophoresis and visualized using the Gel Doc.

Results: 44 normal samples contained genotypes: CC 27.27%, 41.37% CA, AA 11.36%; 70 samples of periodontitis: a light CC 50%, CA 12.5% ​​AA 37.5%; were: CC 38.9%, CA 47.2%, AA 13.9%, by weight: 42.3% CC, CA 30.8%, 19.4% AA.

Conclusion: We found the distribution of the IL-10 genetic polymorphism in the normal group and periodontitis groups, but not related to the severity of periodontitis. (P> 0.05)."

"The Zinc (Zn) concentration was determined from salivary gland of Wistar King A (WKA) rats. The salivary glands are divided in to sub-lingual, sub-mandible and parotis. The Zn concentration was determined within 15-60 minutes of restraint stress. Concentration of Zn in sub-mandible, parotis and sub-Lingual glands was significantly higher (p<0.001) than in the control group. Further more at Zinc concentration in sub-mandible gland at 30 min restraint stress was increase (p<0.001), and decrease significantly at 49 min by restraint stress. By the way, Zn concentration in parotis gland was increase significantly (p<0.0001) after 60 min restraint stress. The results suggest that Zn is linked to oral saliva under physiological stimuli, and that Zn accumulates in the salivary gland during salivary enzyme activity."

"Latar Belakang: Osteoporosis adalah penyakit yang ditandai oleh BMD yang rendah dan mikroarsitektur jaringan tulang yang memburuk akibat kerapuhan tulang yang meningkat dan kerentanan terhadap patah tulang. Beberapa faktor lingkungan dan genetik dianggap dapat berkontribusi terhadap terjadinya penyakit osteoporosis. Salah satu gen yang dapat mempengaruhi proses resorpsi tulang adalah gen LRP5 Gen LRP5 telah terbukti memainkan peran penting dalam biologi tulang. LRP5 adalah protein transmembran dan berfungsi sebagai co-receptor untuk protein Wnt. LRP5 diekspresikan dalam osteoblast dan mempengaruhi pembentukan tulang dengan mengubah Wnt signaling.Tujuan: Penelitian ini bertujuan untuk melihat ada atau tidaknya polimorfisme dan perbedaan polimorfisme gen LRP5 Q89R pada wanita pascamenopause dengan osteoporosis.Metode: 100 bahan biologis tersimpan (50 sampel wanita pascamenopause dengan osteoporosis dan 50 sampel individu sehat) dianalisa menggunakan teknik PCR-RFLP dengan enzim retriksi AvaII, selanjutnya data diuji secara statistik menggunakan uji Chi-square.Hasil: Ditemukan banyak genotip QQ baik pada kelompok osteoporosis dan non-osteoporosis. Pada kelompok osteoporosis terdapat 93% genotip QQ dan 3% genotip QR dan tidak ditemukan genotip RR. Pada kelompok non-osteoporosis, terdapat 100% genotip QQ dan tidak ditemukan genotip QR dan RR.Kesimpulan: Tidak terdapat perbedaan bermakna pada distribusi polimorfisme gen LRP5 Q89R antara penderita osteoporosis dengan kelompok non-osteoporosis (p = 0.105).

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Background: Osteoporosis is a disease characterized by low bone mineral density (BMD) and deteriorating bone tissue microarchitecture due to increased bone fragility and susceptibility to fractures. Some environmental and genetic factors are considered to contribute to the occurrence of osteoporosis. One of the genes that can affect the bone resorption process is the LRP5 gene. The LRP5 gene has been shown to play an important role in bone biology. LRP5 is a transmembran protein and functions as a co-receptor for Wnt protein. LRP5 is expressed in osteoblasts and affects bone formation by changing Wnt signaling.Objective: This research aims to look for genetic polymorphism and differentiate the distribution LRP5 Q89R gene polymorphism in postmenopausal woman with osteoporosis.Methods: 100 stored biological samples (50 samples of postmenopausal woman with osteoporosis and 50 healthy control samples) were analyzed with PCR-RFLP technique using AvaII restriction enzyme, and subsequently assessed with statistical analysis using Chi-square test.Result: QQ genotype was found with the highest amount in both samples. The postmenopausal group has 94% of GG genotype, 6% of QR genotype, and no RR genotype was found. The healthy control group has 100% of GG genotype and no QR and RR genotype was found. Based on Fisher-Extract test, there is no significant association between LRP5 Q89R and postmenopausal osteoporosis (p value = 0.105).Conclusion: The genetic polymorphism of LRP5 Q89R in postmenopausal woman was found, but the polymorphism didnt have any association with osteoporosis in Indonesia populations."

"ABSTRAKLatar belakang: Crossbite merupakan salah satu maloklusi yang sering ditemukan di masyarakat. Crossbite dapat menyebabkan trauma oklusi yang dapat memperberat penyakit periodontal. Masih jarang dijumpai penelitian yang langsung menghubungkan pengaruh crossbite terhadap jaringan periodontal. Tujuan penelitian: Menganalisis hubungan crossbite dengan status periodontal. Metode: Penelitian cross-sectional pada 68 subjek normalbite dan 68 subjek crossbite menggunakan data kartu status rekam medik Klinik Integrasi RSKGM FKG UI tahun kunjungan 20010-2015. Data dianalisis menggunakan uji Mann-Whitney. Hasil: Tidak ada perbedaan bermakna p>0,05 rerata resesi gingiva, kehilangan perlekatan, dan perdarahan gingiva pada subjek normalbite dibandingkan dengan subjek crossbite. Terdapat perbedaan bermakna.

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ABSTRACTBackground Crossbite is one of the most common malocclusion found in the society. Crossbite is a potential cause of trauma from occlusion and can be a cofactor of periodontal diseases. However, research on the effects of crossbite on periodontium is still rare. Objective To analyze the relationship between crossbite and periodontal status. Method A cross sectional study of 68 subjects with normalbite and 68 subjects with crossbite using dental records of patients in Klinik Integrasi RSKGM FKG UI during 2010 2015. Data was statistically analyzed by Mann Whitney test. Result There were no statistically significant differences p 0,05 in the mean values of gingival recession, loss of attachment, and gingival bleeding between normalbite and crossbite groups. However, statistically significant difference."

"ABSTRAKLatar belakang: Terapi regeneratif periodontal GTR memiliki keterbatasan pada defek tulang alveolar satu dinding. Rekayasa jaringan menggunakan teknologi periodontal ligament cell sheet pada chitosan dengan kombinasi molekul adhesif Arginylglycyaspartic Acid RGD diharapkan dapat meningkatkan kadar periostin sebagai indikator regenerasi tulang. Tujuan: Mengevaluasi peningkatan kadar periostin pascaaplikasi RGD pada chitosan-periodontal ligament cell sheet PDLCS . Metode: Aplikasi chitosan-PDLCS dengan penambahan RGD n=3 dan tanpa RGD n=3 pada defek tulang satu dinding yang dibuat pada insisif lateral M. nemestrina. Sampel CKG dikumpulkan setiap minggu selama empat minggu dan disimpan dalam suhu -80 C. Analisis kadar protein menggunakan perangkat ELISA Human POSTN Elabscience. Hasil: Terdapat peningkatan kadar periostin pascaaplikasi RGD pada PDLCS dibandingkan kelompok non-RGD pada minggu pertama dan kedua, dan penurunan kadar periostin pada minggu ketiga dan keempat dengan perbedaan bermakna pada minggu kedua dan keempat p.

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ABSTRACTBackground Periodontal regenerative therapy has limitations on one wall alveolar bone defect. Tissue engineering using periodontal ligament cell sheet on chitosan addition of adhesive molecule Arginylglycyaspartic Acid RGD is expected to increase periostin levels as an indicator of bone regeneration. Objective To see levels of periostin post application of RGD on chitosan periodontal ligament cell sheet PDLCS . Method Application of chitosan PDLCS with addition of RGD n 3 and without RGD n 3 on one wall bone defect made on the lateral incisor of M. nemestrina. The CKG sample was collected weekly for four weeks and stored at 80 C. Analysis of protein content using ELISA Human POSTN Elabscience. Results Periostin level was increased in RGD PDLCS compared to non RGD groups in the first and second weeks, and decreased periostin levels in the third and fourth weeks with significant differences in second and fourth weeks p "

"Latar belakang: Murine double minute 2 MDM2 merupakan regulator negatif p53. Gen ini memiliki peran penting dalam meregulasi tingkat dan aktivitas p53, yang merupakan tumor supresor. Polimorfisme gen MDM2 SNP309 dengan perubahan basa T menjadi G dilaporkan meningkatkan suseptibilitas kanker kepala leher KKL. Tujuan: Penelitian ini bertujuan untuk melihat pola distribusi polimorfisme gen MDM2 SNP309 pada penderita KKL dan individu sehat di populasi Indonesia. Metode: Teknik PCR-RFLP dilakukan untuk mengidentifikasi polimorfisme gen MDM2 SNP309 pada 50 sampel penderita KKL dan 50 individu sehat. Dilakukan analisis statistik dengan uji Fisher exact untuk melihat perbedaan distribusi polimorfisme gen MDM2 SNP309 pada kedua kelompok. Hasil: Penelitian ini menunjukkan persentase genotip polimorfisme sebesar 70 pada sampel KKL dan 80 pada sampel kontrol. Kesimpulan: Polimorfisme gen MDM2 SNP309 ditemukan pada kedua kelompok, namun tidak terdapat perbedaan bermakna distribusi polimorfisme gen MDM2 SNP309 pada penderita KKL dan individu sehat p = 0,356.

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Background: Murine double minute 2 MDM2 is negative regulator of p53. This gene plays a critical role by down regulating tumor suppressor p53 level and activity. Polymorphism of MDM2 SNP309 with T to G change has been reported to increase the susceptibility of head and neck cancer HNC.

Aim: This study aimed to find relationship and distribution of MDM2 SNP309 genetic polymorphism in HNC patients and controls in Indonesian population.

Method: PCR RFLP technique is used to identify the polymorphism in 50 HNC patients and 50 healthy individuals. A statistical analysis with Fisher exact test is used to see the difference of genetic polymorphism of MDM2 SNP309 distribution in both groups.

Result: This research showed the percentage of polymorphism genotype is 70 in head and neck cancer samples and 80 in healthy individual samples.

Conclusion: This study found MDM2 SNP309 genetic polymorphism in both groups, but there is no significant distribution difference between head and neck cancer patients and healthy individuals p 0,356."

"Latar Belakang: Faktor transkripsi Hypoxia inducible factor-1 (HIF-1 merupakanpengatur utama hipoksia, termasuk menyebabkan penekanan sistem perbaikan deoxyribose nucleic acid (DNA), sehingga menghasilkan instabilitas genetik pada sel kanker. Varian genetik HIF-1α C1772T (P582S) dan G1790A (A588T) dipercaya mempunyai aktivitas transkripsi yang lebih tinggi.Peranan polimorfisme HIF-1α ini sudah diteliti pada beberapa jenis kanker seperti kanker ginjal, payudara, ovarium, tetapi belum ada penelitian pada kanker paru. Metode: Polimorfisme HIF-1α diperiksa dengan menggunakan direct sequencing dengan total sampel 83 pasien kanker paru (42 adenokarsinoma, 30 skuamous sel karsinoma, empat adenoskuamous sel karsinoma dan tujuh kanker paru karsinoma sel kecil (KPKSK) dan 110 subjek sehat sebagai kontrol. Hubungan polimorfisme HIF-1α dengan kelainan genetik/epigentik loss of heterozygot (LOH) TP53, LOH 1p34, LOH retinoblastoma-1 (RB1), inaktivasi p16 dan kelainan epidermal growth factor receptor (EGFR) kemudian diperiksa. Hasil: Frekuensi polimorfisme HIF-1α pada kanker paru dan kontrol telah sesuai dengan keseimbangan Hardy-Weinberg. Pada penelitian ini tidak ditemukan perbedaan frekuensi genotipe C1772T atau G1790A antara kanker paru dengan kontrol sehat. Tetapi, frekuensi varian HIF1A C1772T ditemukan tinggi bermakna di pasien kanker paru dengan LOH TP53 (p=0,015). Pada pasien adenokarsinoma, individu dengan varian alel memiliki frekuensi tinggi LOH TP53 (p=0,047), LOH 1p34 (p=0,009) atau keduanya (LOH TP53 dan LOH 1p34) (p=0,008). Aktivitas transkripsi juga diperiksa secara in vitro dan ditemukan HIF1A varian pada sel kanker paru A549 mempunyai aktivitas yang meningkat secara bermakna dibanding wild type HI1F1A baik di kondisi normoksia atau hipoksia, terutama P582A di sel dengan mutan p53 (p< 0,0005 dan p< 0,005). Kesimpulan: Penelitian ini mengindikasikan polimorfisme gen HIF-1α mempunyai peranan penting dalam karsinogenesis paru terutama pada adenokarsinoma, diduga melalui peningkatan instabilitas genetik.

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Background and objective: The transcription factor, hypoxia-inducible factor-1 (HIF-1), is a master regulator of hypoxia, including repression of DNA repair systems, resulting in genomic instability in cancer cells. The roles of the polymorphic HIF-1a variants, C1772T (P582S) and G1790A (A588T), which are known to enhance transcriptional activity, were evaluated in lung cancers.

Methods: HIF-1a polymorphisms were assessed by direct sequencing in a total of 83 lung cancer patients (42 adenocarcinomas, 30 squamous cell, four adenosquamous cell and seven small cell lung carcinomas) and in 110 healthy control subjects. The relationship between these polymorphisms and the frequently observed genetic and/or epigenetic aberrations, TP53 loss of heterozygosity (LOH), 1p34 LOH, retinoblastoma-1 (RB1) LOH, p16 inactivation and epidermal growth factor receptor aberrations, was then assessed.

Results: There were no significant differences in genotype frequencies for either C1772T or G1790A between lung cancer patients and healthy controls. However, the frequency of the HIF1A C1772T variant allele was significantly higher in lung cancer patients with TP53 LOH (P = 0.015). Among adenocarcinoma patients, individuals with variant alleles of either polymorphism showed significantly higher frequencies of TP53 LOH (P = 0.047), 1p34 LOH (P = 0.009), or either of these (P = 0.008) in the tumours. The in vitro transcriptional activity of these HIF1A variants in A549 lung cancer cells was significantly greater than that of the wild type under either normoxic or hypoxic conditions, especially for P582S in cells containing mutant p53 (P < 0.0005 and P < 0.005, respectively).

Conclusions: These findings indicate that functional polymorphisms in the HIF-1a gene may have an important impact on lung carcinogenesis, especially in adenocarcinomas, possibly by increasing genomic instability."