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"Keragaman dan Komposisi Asam Lemak Bakteri Lipolitik Isolat Asal Tanah dan Sedimen Perairan Wilayah Hutan dan Perkebunan Sawit. Bakteri spesifik dalam hutan dan perkebunan kelapa sawit adalah bakteri lipolitik. Enzim-enzim dari bakteri lipolitik telah diaplikasikan di agro-akuakultur, makanan, deterjen, farmasi, industri susu, dan biodiesel-biokerosin. Penelitian bertujuan memberikan informasi keragaman bakteri lipolitik asal tanah dan sedimen perairan di areal hutan dan perkebunan kelapa sawit, dan komposisi asam lemaknya. Sampel tanah berasal dari tanah lapisan atas hutan dataran rendah dan perkebunan kelapa sawit dan sedimen air tawar di dekat situs tersebut. Hutan tersebut terletak di Taman Nasional Bukit Duabelas dan perkebunan kelapa sawit sekitarnya di Kabupaten Sarolangun, Jambi, Indonesia. Sebanyak 22 isolat terpilih dari tiga puluh dua isolat bakteri lipolitik yang tumbuh pada media selektif lipolitik, terdiri dari 11 isolat dari tanah lapisan atas dan 11 isolat dari sedimen air di hutan dan daerah perkebunan. Isolat-isolat bakteri diidentifikasi berdasarkan analisis gen 16S rRNA. Hasil identifikasi menunjukkan isolat-isolat tersebut terdiri atas lima genera yaitu Burkholderia, Cupriavidus, Serratia, Acinetobacter, dan Kurthia. Pada pohon filogenetik yang dibangun menggunakan metode maximum likelihood isolat-isolat tersebut terdistribusi ke dalam tiga grup, yaitu grup Burkholderia-Cupriavidus, grup Serratia-Acinetobacter, dan grup Kurthia. Hasil analisis kromatografi gas (GC-FID) menunjukkan bahwa enzim lipolitik yang dihasilkan bakteri-bakteri tersebut terdiri atas berbagai asam lemak. Beberapa isolat bakteri menghasilkan asam lemak esensial, seperti asam lemak: linoleat, linolenat, arakidonat, eikosapentanoat (EPA), dan dokosaheksanoat (DHA).

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The specific bacteria in forests and on oil palm plantations are lipolytic bacteria. Their enzymes have been applied in the agro-aquaculture, food, detergent, pharmaceutical, dairy, and biodiesel-biokerosene industries. This study describes the diversity of cultivable lipolytic bacteria from soil and aquatic sediment in a forest and on an oil palm plantation and their fatty acid products. Soil samples used in this research were obtained from topsoil in a lowland forest and on an oil palm plantation and from sediments in fresh water near these sites. The forest is located in Bukit Duabelas National Park, and the oil palm plantation is near the forest in Sarolangun District, Jambi Province, Indonesia. Twenty-two isolates of lypolitic bacteria were selected from 32 isolates grown in lipolytic selective medium. The 22 consisted of 11 isolates from topsoil and 11 from aquatic sediment from the forest and plantation area. These isolates were identified by 16S rRNA-sequence data analysis. Taxonomically, they belonged to five genera: Burkholderia, Cupriavidus, Serratia, Acinetobacter, and Kurthia. The maximum likelihood tree showed that they are phylogenetically distributed in three clusters. They were clustered into three groups: the Burkholderia-Cupriavidus group, the Serratia-Acinetobacter group, and the Kurthia group. Their lipolytic enzymes formed various fatty acids after analysis by gas chromatography-flame ionization detector (GC-FID). Some isolates formed essential fatty acids, such as linoleic, linolenic, arachidonic, eicosapentanoic acid (EPA), and docosahexanoic acid (DHA)."

"ABSTRACTThe Oil derrived from the oil palm plant has become one of the most contested agricultural commodities in Southeast Asia, and development of novel uses for it is in high demand. Trehalose fatty acid esters have begun attracting attention due to their specific potent applications (based on the exceptional intrinsic properties of the hydrophilic trehalose part) and their amphiphilicity. In order to enhance the usability of palm products and trehalose fatty acid esters, in this present study we focused on the use of trehose fatty acid monoesters (TMEs) prepared with natural fatty acids extracted from oil palms as cryoprotectants. "

"Deep Eutectic Solvent (DES) merupakan solven yang dapat dijadikan sebagai pelarut ekstraksi untuk deasidifikasi asam lemak bebas dari minyak inti sawit. Pelarut tersebut memiliki toksisitas rendah dan tidak mereduksi kandungan senyawa anti-oksidan di PKO/CPKO. Penelitian ekstraksi asam lemak bebas menggunakan DES berbasis kolin klorida dan polialkohol telah dibuktikan untuk mengekstraksi asam palmitat pada minyak sawit. Tujuan penelitian ini adalah untuk menggunakan DES berbasis kolin klorida dengan HBD 1,2-hexandiol dengan berbagai rasio molar terhadap kolin klorida untuk meninjau kelarutannya terhadap asam laurat pada PKO. Selain itu, melakukan ekstraksi asam laurat pada PKO untuk mendapatkan jumlah tahapan ekstraksi untuk mencapai mutu PKO asam lemak bebas dibawah 0.3%. Kemudian, perbedaan kelarutan asam lemak bebas yang berbeda pada minyak dalam DES pun ditinjau. Hasil penapisan menunjukan DES terbaik dengan HBA kolin klorida dan HBD 1,2 heksandiol dengan rasio molar 1:8 yang dapat memisahkan asam lemak bebas dari model minyak inti sawit dengan efisiensi ekstraksi maksimum 61.0%. Selain itu, dibutuhkan 4 jumlah tahapan ekstraksi agar kandungan asam lemak bebas pada model minyak inti sawit di bawah 0.3% mutu SNI 2012. Hasil dari penelitian menunjukan perbedaan efisiensi DES dalam mengekstraksi asam lemak bebas yang berbeda dalam minyak inti sawit, dimana bertambahnya 2 jumlah atom karbon pada asam dapat menyebabkan penurunan yield ekstraksi DES sebesar 11%.

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Deep Eutectic Solvent (DES) is a solvent that can be used for the extraction of free fatty acids from palm kernel oil. This solvent has low toxicity and does not reduce the content of antioxidant compounds in PKO/CPKO. Research on the extraction of free fatty acids using DES based on choline chloride and polyalcohol has been proven effective in extracting palmitic acid from palm oil. The objective of this study is to use choline chloride-based DES with 1,2-hexanediol as the hydrogen bond donor (HBD) at various molar ratios to examine its solubility towards lauric acid in PKO. Additionally, the extraction of lauric acid from PKO is performed to determine the number of extraction stages required to achieve a free fatty acid content in PKO below 0.3%. Furthermore, the interaction between DES and different fatty acid contents in the oil is investigated. The screening results identified the best DES with choline chloride as the hydrogen bond acceptor (HBA) and 1,2-hexanediol as the HBD, at a molar ratio of 1:8, which effectively separated free fatty acids from a palm kernel oil model with a maximum extraction efficiency of 61.0%. Moreover, it was found that four extraction stages were required to reduce the free fatty acid content in the palm kernel oil model below the 0.3% threshold specified by the SNI 2012 quality standard. Different interactions of DES in extracting different free fatty acids from palm kernel oil were observed, where an increase of two carbon atoms in the fatty acid led to an 11% decrease in the extraction yield of DES."

"Senyawa ester sukrosa asam lemak telah berhasil disintesis melalui reaksi esterfikasi antara dua variasi asam lemak yaitu asam lemak hasil hidrolisis minyak kelapa sawit dan asam palmitat dengan sukrosa secara enzimatis menggunakan enzim lipase Candida rugosa. Optimasi reaksi dilakukan pada rasio molar antara sukrosa dan asam lemak, yaitu 1:0,2 ; 1:0,4 ; 1:0,6 dan 1:0,8. Pelarut yang digunakan adalah n-heksana dengan rasio 1:1 v/v substrat. Berat enzim yang digunakan adalah 5 dari total berat substrat. Kondisi optimum pembentukan ester sukrosa palmitat dan ester sukrosa asam lemak hasil hidrolisat minyak kelapa sawit diperoleh pada rasio 1:0,8 berturut ndash; turut, yaitu sebesar 38,23 dan 24,15. Identifikasi menggunakan instrument FTIR menunjukan pita serapan yang khas untuk gugus ester sukrosa palmitat dan ester sukrosa asam lemak hasil hidrolisat berturut ndash; turut, yaitu 1735 cm-1 dan 1734 cm-1. Ester sukrosa palmitat dan ester sukrosa hidrolisat memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus tetapi tidak untuk bakteri Eschericia coli. Aktivitas hambatan terbesar terhadap bakteri S.aureus ditemukan pada rasio molar 1:0,2. Diameter hambat terbesar untuk ester sukrosa palmitat sebesar 13 mm, sedangkan ester sukrosa hidrolisat sebesar 10 mm.

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The fatty acid sucrose ester compound has been successfully synthesized through an esterification reaction between the two variations of fatty acids is fatty acid hydrolysis of palm oil and palmitic acid with sucrose enzymatically using Candida rugosa lipase enzyme. Reaction optimization was performed on variations in the molar ratio between sucrose and fatty acid 1 0.2 1 0.4 1 0.6 and 1 0.8. The solvent used was n hexane with a ratio of 1 1 v v substrates. The weight of the enzyme used is 5 of the total weight of the substrate. The optimum conditions of sucrose palmitate ester formation and fatty acid sucrose hydrolyzate acid esters were obtained at a ratio of 1 0.8, 38.23 and 24.15, respectively. The identification using FTIR indicates a typical absorption band for the C O ester group at the 1735 cm 1 wave number for the sucrose palmitate ester and 1734 cm 1 for the sucrose fatty acid sucrose ester. The palmitate sucrose ester and sucrose hydrolyzate ester have the ability to inhibit the growth of Staphylococcus aureus bacteria but not Eschericia coli bacteria. The largest inhibitory activity against Staphylococcus aureus bacteria was found in a 1 0.2 molar ratio with the largest inhibitory diameter for a palmitate sucrose ester of 13 mm, while a sucrose hydrolyzate ester of 10 mm."

"Asam suksinat dapat diproduksi dari tandan kosong kelapa sawit (TKKS) melalui proses fermentasi. Pada penelitian ini, produksi asam suksinat dilakukan menggunakan isolat bakteri dari rumen sapi melalui metode Semi Simultaneous Saccharification and Fermentation (SSSF). Isolat bakteri dari cairan rumen sapi diperoleh dengan melakukan tahap isolasi terlebih dahulu. Tahapan isolasi dilakukan dengan melakukan enrichment, subkultur, isolasi, dan fermentasi bakteri. TKKS sebagai sumber karbon, juga dilakukan tahap pretreatment terlebih dahulu menggunakan larutan peracetic acid dan alkaline peroxide serta tahap prehidrolisis menggunakan enzim selulase untuk menghasilkan glukosa. Tahap SSSF dilakukan dengan konsentrasi awal glukosa yang berbeda, yaitu 0,45; 0,48; dan 0,61 g/L. Berdasarkan hasil yang diperoleh, konsentrasi, yield, dan produktivitas asam suksinat tertinggi sebesar 3,12 g/L, 0,312 g/g TKKS, dan 0,13 g/L/jam, secara berurutan, diperoleh pada konsentrasi awal glukosa sebesar 0,61 g/L. Selain itu, berat kering bakteri dan konversi glukosa tertinggi sebesar 0,0775 gr dan 73,61 %, secara berurutan, juga diperoleh pada konsentrasi awal glukosa sebesar 0,61 g/L. Estimasi parameter kinetika pertumbuhan bakteri juga dilakukan dalam penelitian ini. Berdasarkan perhitungan, laju pertumbuhan spesifik tertinggi sebesar 0,051 jam^-1 diperoleh pada konsentrasi awal glukosa sebesar 0,61 g/L.

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Succinic acid can be produced from oil palm empty fruit bunches (OPEFB) through a fermentation process. In this study, succinic acid production was carried out using bacteria isolated from cattle rumen through the Semi Simultaneous Saccharification and Fermentation (SSSF) method. Bacteria were isolated from cattle rumen fluid by doing the isolation stage first. The stages of isolation were carried out by doing enrichment, subculture, isolation, and fermentation of bacteria originated. OPEFB as a carbon source, were pretreated through pretreatment stage using peracetic acid and alkaline peroxide solution and then continue to the prehydrolysis stage using cellulase enzymes in order to produce glucose. The SSSF stage was carried out with different initial glucose concentrations, which are 0.45; 0.48; and 0.61 g/L.

Based on the results obtained, the highest concentration, yield, and productivity of succinic acid of 3.12 g/L, 0,312 g/g EFB, and 0.13 g/L/h, respectively, were obtained at the initial glucose concentration of 0.61 g/L. In addition, the highest dry weight of bacteria and glucose conversion were 0.0775 gr and 73.61 %, respectively, were also obtained at the initial glucose concentration of 0.61 g/L. Estimation of bacterial growth kinetics parameters was also carried out in this study. Based on calculations, the highest specific growth rate of 0.051 h^-1 was obtained at the initial glucose concentration of 0.61 g/L."

"Penelitian ini bertujuan untuk melihat pengaruh dari harga CPO terhadap alih fungsi pada hutan produksi konversi (HPK) menjadi perkebunan sawit di Indonesia, dengan adanya proses pelepasan kawasan hutan untuk perkebunan sawit. Dengan menggunakan data pelepasan kawasan hutan untuk perkebunan sawit di tingkat provinsi tahun 1995 sampai tahun 2017, pelepasan kawasan hutan diestimasi menggunakan model tobit dengan left-censored (0), serta variabel ekonomi yang mempengaruhi permintaan lahan untuk sawit yaitu harga CPO, tingkat suku bunga, nilai tukar, serta besarnya share industri pengolahan dalam Produk Domestik Bruto (PDB), dan GDP dunia. Hasil empiris menujukkan bahwa tingkat harga pada lag tertentu secara signifikan mempengaruhi peningkatan pelepasan kawasan hutan untuk perkebunan sawit sebesar 23,55% dan 23,48%. Penelitian ini menemukan bahwa harga CPO berperan besar dalam pelepasan kawasan hutan untuk perkebunan sawit di Indonesia di masing-masing wilayah.

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This study aims to look at the effect of CPO prices on the conversion in conversion production forests (HPK) to oil palm plantations in Indonesia, with the process of releasing forest areas for oil palm plantations. Using data on the release of forest areas for oil palm plantations at the provincial level from 1995 to 2017, the release of forest areas was estimated using the Tobit model with left-censored (0), as well as economic variables that affect land demand for oil palm, namely CPO prices, interest rates, values exchange rates, as well as the size of the share of the manufacturing industry in the Gross Domestic Product (GDP), and world GDP. Empirical results show that the price level at a certain lag significantly influences the increase in the release of forest areas for oil palm plantations by 23.55% and 23.48%.This study found that the price of CPO played a major role in the release of forest areas for oil palm plantations in Indonesia in each region."

"AbstrakThe chief purpose of this paper is to analyze the formation and growth of oil palm plantation enterprises in postcolonial Indonesia. Most of the oil palm plantation enterprises in Indonesia are incorporated businesses organized as limited companies (perseroan terbatas, PT). Amendments of the corporate charters of limited companies concerning their formation, increase in capital, executive appointments, and so on must be reported to the government. Each amendment is announced by an Appendix of the State Gazette (Tambahan Berita Negara Republik Indonesia, TBN RI), which is annually printed, bound, and stored by the State Printing Office (Percetakan Negara Republik Indonesia, PNRI). Using data from these official documents for the period until 1999, this article presents an outline of the development of oil palm plantation enterprises mainly in the 1980s and 1990s. It contains the profiles of top class business groups such as Asian Agri, Astra Agro, Salim (Indoagri), and Sinar Mas, as well as some second class groups among the nearly 500 corporate enterprises that were engaged in the oil palm plantation business."

"ABSTRAKReduction of free fatty acid (FFA) in used palm oil was determined after treatment of waste cooking oil with an adsorbent derived from coffee bean husk ash. Coffee husks were burned at 600 ºC for 12 hrs to obtain the adsorbent ash. Free fatty acid removal efficiency was optimized with respect to ash dosage, contact time and temperature. It was found that shaking ash (1 g) with waste palm oil (50 g) at 250 rpm and 30°C for 330 min gave the highest reduction in free fatty acids (FFA) (1966 mg/g). The adsorption isotherm was followed by Temkin (R2 = 0.9283) and Freundlich models (R2 = 0.9146). The adsorption of FFA at all adsorbent doses followed pseudo-second order kinetics (R2 = 0.9817-0.9999). A thermodynamic study revealed that the changes in enthalpy, entropy and Gibbs free energy were 89.07 kJ/mol, -0.25 kJ/mol.K and -5.15 to -12.17 kJ/mol, respectively. The coffee husk ash (5 g ash / 50 g waste palm oil) was found to reduce FFA by 100% at 30°C.KeywordsCoffee husk, Used palm oil, Free fatty acid, Adsorption, Ash"

"Penambahan substrat sampah makanan dan limbah minyak dan lemak berpotensi menghasilkan akumulasi VFA yang berdampak kepada ketidakseimbangan proses AD. Penelitian ini bertujuan untuk menentukan kesesuaian limbah minyak dan lemak dengan sampah makanan dan kotoran sapi yang dioperasikan menggunakan AD terhadap parameter kualitas efluen dengan mengidentifikasi hubungan antara akumulasi VFA dan LCFA dalam pembentukan biogas dan VSD. Penelitian ini dilakukan selama 245 hari menggunakan Continuous Stirred Tank Reactor CSTR dengan kapasitas 400 L dengan rata-rata suhu 29 0,90 C dan pH 6,2 0,71. Reaktor dioperasikan dengan Organic Loading Rate OLR 10 Kg VS/m3.2-3hari dan pengadukan 30 rpm selama 4 jam/hari. Penelitian terdiri dari tiga variasi, yang terdiri dari variasi 1 sampah makanan dan kotoran sapi dengan perbandingan 9:1, variasi 2 limbah minyak dan lemak dengan sampah makanan dan kotoran sapi dengan perbandingan 1:7, dan variasi 3 limbah minyak dan lemak dengan sampah makanan dan kotoran sapi dengan perbandingan 1:2. Pada kondisi steady state, Variasi 1 menunjukan rata-rata reduksi COD 54,24 7,11 ; VSD 75,8 6,47 ; amonia 1,17 6,74 ; dan pH 6,0 3,87. Penambahan limbah minyak dan lemak dengan variasi 2 menunjukan hasil pengujian yang berbeda secara signifikan dengan variasi 1 yang ditandai dengan uji test-T dengan tingkat kepercayaan 95. Hasil pengujian varasi 2 menunjukan rata-rata reduksi COD mencapai 57,6 6,9 ; VSD 88,9 5,49 ; amonia 1,08 11,55 ; pH 5,82 3,6 , dan rasio VFA/TA akhir proses dengan nilai 0,36 yang menunjukan proses AD berjalan stabil dan tidak ada akumulasi VFA dan LCFA. Sedangkan variasi 3 lebih efisien dalam mengolah limbah minyak dan lemak menggunakan reaktor AD sampah makanan yang dibuktikan dengan reduksi COD diatas 50 dengan range 52,21 ndash; 68,27, rata-rata VSD 93,46, rasio VFA/TA akhir proses 0,69 yang menunjukan ada beberapa ketidakstabilan akan terjadi pada proses AD, akan tetapi, tetap tidak terjadi akumulasi VFA pada dan LCFA pada variasi 3, serta menghasilkan volume biogas tertinggi hingga 980 L/hari.

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The addition of food waste substrate and waste oils and fats has the potential to produce VFA accumulation which has an impact on the imbalance of the AD process. This research was aimed to determine the suitability between of fat, oil, and grease FOG wastes, and food waste and cow mannure that was operated using AD on effluent quality parameter by identifying the relationship between VFA and LCFA accumulation in the formation of biogas and VSD. This research was conducted for 245 days used Continuous Stirred Tank Reactor CSTR with it area of capacity 400 by its averages temperature and pH, were 29 0,90 C and pH 6,2 0,71, respectively. To operate the reactor using Organic Loading Rate OLR 10 Kg VS m3.2 3day and steering 30 rpm for 4 hours day. The study consisted of three variations, consisting of variation 1 food waste and cow mannure in the ratio of 9 1 , variation 2 waste of fat, oil, and grease with food waste and cow mannure in the ratio of 1 7 , and variation 3 waste of fat, oil, and grease with food waste and cow mannure with a ratio of 1 2. In steady state conditions, Variation 1 shows the average COD reduction reached 54,24 7,11 VSD 75.81 6,47 ammonia 1,17 6,74 and pH 6,0 3,87, respectively. The addition of FOG waste with variation 2 have significantly different test results with variation 1 characterized by a test T with a 95 confidence level. The test results of variation 2 obtained that the average reduction of COD reached 57,6 6,9 VSD 88,9 5,49 ammonia 1,08 11,55 pH 5,82 3,6, and a final process VFA TA ratio of 0,36 indicating that the AD process is stable and there is no accumulation of VFA and LCFA, respectively. While the addition of FOG waste variation 3 more efficient in treating FOG waste using AD reagent food waste as evidenced by the reduction of COD above 50 with a range of 52.21 68.27 and average VSD 93.46, a final process VFA TA ratio of 0,69 indicates that some instability will occur in the AD process, however, there is no VFA accumulation in and LCFA in variation 3 with produce highest biogas volume up to 980 L day. "