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"This thesis presents a novel method for fabricating the smallest superconducting quantum interference device (SQUID) that resides on the apex of a very sharp tip. The nanoSQUID-on-tip displays a characteristic size down to 100 nm and a field sensitivity of 10^-3 Gauss/Hz^(1/2). A scanning SQUID microsope was constructed by gluing the nanoSQUID-on-tip to a quartz tuning-fork. This enabled the nanoSQUID to be scanned within nanometers of the sample surface, providing simultaneous images of sample topography and the magnetic field distribution. This microscope represents a significant improvement over the existing scanning SQUID techniques and is expected to be able to image the spin of a single electron."

"A nonconductor optical technique, having lateral resolution of about 5 μm and vertical resolution of about 0.09 μm, for surface profile (roughness) measurement was studied. It based on a heterodyne interferometer in which two orthogonal polarized beams of slightly different frequencies were used in a modified interference microscope. The beams scanned the surface of a work piece, and the reflected beams were allowed to interfere with one another. The phase of the beat frequency of the interfering return beams is directly proportional to the surface height. The result of a surface measurement include graphical displays of surface profile, roughness (Ra), root mean square (rms) and peak to valley (P-V) value."

"Kondensasi kromosom pada tahap metafase memiliki peranan penting dalam keberlangsungan siklus sel. Sejauh ini masih terus dilakukan penelitian mengenai faktor yang mempengaruhi kondensasi kromosom. Kation divalen, terutama kalsium (Ca2+) diketahui memiliki peranan penting dalam kondensasi kromosom manusia. Akan tetapi, peranan Ca2+ terhadap kromosom tumbuhan belum diketahui. Gandum merupakan salah satu organisme model dalam sitogenetika. Oleh karena itu, diperlukan penelitian mengenai efek Ca2+ kalsium terhadap kondensasi kromsosom gandum (Triticum spp.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian 1 mM BAPTA (1, 2-bis(2-aminophenoxy) ethane-N, N, N′, N′-tetraacetic acid), 1 mM EDTA (Ethylenediaminetetraacetic acid), sebagai pelekat Ca2+, terhadap struktur kromosom gandum menggunakan mikroskop cahaya dan Scanning Electron Microscope (SEM). Kecambah gandum dipotong bagian ujung akar dan diinkubasi di dalam larutan PFA (Paraformaldehyde) 2%. Sampel diinkubasi dengan enzim selulase 2,5 % dan pectoliase 2,5%. Setelah itu sampel diberi perlakuan masing-masing dengan 1 mM BAPTA, 1 mM EDTA, dan kontrol dengan PBS. Kromosom kemudian diwarnai dengan aceto orcein sedangkan yang diamati SEM dipreparasi melalui tahap fiksasi, post-fiksasi, staining, dehidrasi, dan dikeringkan dengan HMDS (hexamethyldisilazane) sebelum diamati menggunakan SEM. Hasil yang diperoleh pada pengamatan kuantitatif menunjukkan panjang rata-rata untuk kromosom kontrol 12,5±3,4 µm sedangkan pada perlakuan 1 mM BAPTA dan 1 mM EDTA panjang rata-rata yang diperoleh adalah 17,7±6,6 µm dan 41,9±16,3 µm. Struktur kromosom kontrol secara kualitatif lebih padat dan lebih terkondensasi. Akan tetapi kromosom yang diberi perlakuan 1 mM BAPTA dan 1 mM EDTA menunjukkan hasil yang lebih panjang, tersebar, dan terjadi dekondensasi kromosom sehingga serat-serat kromatin lebih terlihat jelas. Hal tersebut menunjukkan Ca2+ memiliki peranan penting dalam kondensasi kromosom gandum.

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Chromosome condensation at metaphase has an important role in the continuation of the cell cycle. Researches on the investigation of the major factors affecting chromosome condensation has been conducted so far. Divalent cations, especially Ca2+ has been reported to have an important role in human chromosome condensation. Nevertheless, its effect on the plant chromosome has yet to be evaluated. Wheat (Triticum spp.) is one of the model organisms in cytogenetics.Therefore, study to evaluate the effect of Ca2+ on wheat chromsosomes is required. The purpose of this study was to determine the effect of 1 mM BAPTA and 1 mM EDTA as the Ca2+ chelating agent on the wheat chromosomes using light microscope and SEM (Scanning Electron Microscope). The root tips of wheat sprouts was cut off and incubated in a 2% PFA (Paraformaldehyde)  solution. The sample was incubated with 2.5% cellulase and 2.5% pectoliase enzyme. After that, the samples were treated with 1 mM BAPTA, 1 mM EDTA, and PBS as control. Chromosome were then stained with aceto orcein, while sample for SEM were subjected to SEM preparation including fixation, post-fixtaion, staining, dehydration, and HMDS (hexamethyldisilazane)  drying. The results showed that the average length of the control chromosome was 12.5±3.4 µm, while those treated with 1 mM BAPTA and 1 mM EDTA showed the average length of 17.7 ± 6.6 µm and 41. 9 ± 16.3 µm, respectively. The qualitative observation of the control chromosomes showed the more compact and condensed structure. However, chromosomes treated with 1 mM BAPTA and 1 mM EDTA showed a longer, dispered, and decondensed chromosome showing the chromatin fibers. This result indicated that Ca2+ play an important role in wheat chromosome condensation."

"Kromosom memiliki peranan penting dalam menyimpan materi genetik setiap makhluk hidup. Salah satu faktor yang memengaruhi struktur kromosom adalah kation divalen termasuk ion tembaga (Cu2+). Hingga saat ini, belum terdapat penelitian yang menganalisis pengaruh Cu2+ terhadap struktur kromosom tumbuhan. Tumbuhan gandum (Triticum aestivum) merupakan organisme model terkait studi kromosom. Tujuan penelitian ini adalah mengetahui pengaruh Cu2+ pada berbagai konsentrasi (0, 20, 40, 80, 160, dan 1000 µM) terhadap struktur kromosom gandum menggunakan mikroskop cahaya dan scanning electron microscope (SEM). Biji gandum direndam di larutan Cu2+ selama 4 jam, kemudian dikecambahkan selama 48 jam. Ujung akar gandum kemudian disinkronisasi dengan kolkisin 0,01% dan difiksasi dengan larutan Carnoy. Sampel yang akan diamati menggunakan mikroskop cahaya kemudian dihidrolisis dengan HCl 1N, diwarnai dengan aceto-orcein, dan diamati di bawah mikroskop. Sampel yang akan diamati menggunakan SEM diberi enzim 2,5% selulase-pektinase, difiksasi menggunakan 2,5% glutaraldehid, post-fiksasi menggunakan OsO4, dehidrasi dengan etanol, dikeringkan dengan HMDS, dan diamati di bawah SEM. Hasil kualitatif menunjukkan struktur kromosom mulai dari yang paling terkondensasi hingga tidak terkondensasi berturut-turut adalah 20, 80, 40, 1000, 160, dan 0 µM Cu2+. Hasil kuantitatif menunjukkan panjang+lebar kromosom dari setiap perlakuan 0, 20, 40, 80, 160, dan 1000 µM Cu2+ berturut-turut adalah 18,378+1,676 µm, 11,484+2,028 µm, 16,035+1,765 µm, 15,402+1,791 µm, 17,427+1,550 µm, dan 16,321+1,500 µm. Uji perbandingan Tukey dan Dunn menunjukkan bahwa konsentrasi Cu2+ memberi pengaruh yang signifikan terhadap panjang dan lebar kromosom, khususnya pada konsentrasi 20 µM. Hasil dari penelitian ini menyatakan bahwa Cu2+ memiliki peran penting dalam menjaga struktur kromosom gandum.

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Chromosomes have an important role in storing the genetic material of every living creature. One of various factors that can affect the structure of chromosomes is divalent cations including copper ions (Cu2+). Until now, no research has analysed the effect of Cu2+ on plant chromosome structure. Wheat plants (Triticum aestivum) are a model organisms related to the study of chromosomes. This study aimed to determine the effect of Cu2+ at various concentrations (0, 20, 40, 80, 160, and 1000 µM) on wheat chromosome structure using a light microscope and scanning electron microscope (SEM). Wheat seeds were soaked in each Cu2+ solution for 4 hours and germinated for 48 hours. Root tips were then synchronized in colchicine 0.01% and fixed with Carnoy’s solution. For light microscope observation, the root tips were hydrolysed with 1N HCl, stained with aceto-orcein, and observed under the microscope. For SEM observation, the root tips were then treated with 2,5% cellulase-pectinase enzyme, fixed using glutaraldehyde, post-fixed using OsO4, dehydration using ethanol, dried with HMDS, and observed under SEM. The qualitative results showed that the chromosomes structure from most condensed to less condensed were 20, 80, 40, 1000, 160, and 0 µM Cu2+ respectively. The quantitative results showed that the length+width of the chromosomes from each treatment of 0, 20, 40, 80, 160, and 1000 µM Cu2+ were 18,378+1,676 µm, 11,484+2,028 µm, 16,035+1,765 µm, 15,402+1,791 µm, 17,427+1,550 µm, and 16,321+1,500 µm respectively. Tukey and Dunn comparison test showed that the concentration of Cu2+ significantly affected the length and width of chromosomes, especially at 20 µM concentration. The result of our study indicates that Cu2+ has an important role in maintaining the structure of wheat chromosomes.

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"Kromosom merupakan substansi genetik pada makhluk hidup yang diwariskan ke generasi selanjutnya. Kromosom terbentuk melalui proses kondensasi selama siklus sel. Faktor-faktor yang mempengaruhi kondensasi kromosom adalah protein scaffold dan kation divalen, terutama ion kalsium (Ca2+). Peran ion kalsium terhadap kromosom masih terbatas pada kromosom manusia dan belum pernah dilaporkan pada kromosom tumbuhan. Tujuan penelitian ini untuk mengetahui peran ion kalsium terhadap struktur kromosom barley menggunakan mikroskop fluoresens dan scanning electron microscope. Kromosom barley diisolasi kemudian diberi perlakuan 1 mM BAPTA sebagai agen pengikat ion kalsium dan PBS sebagai kontrol. Terdapat perbedaan panjang dan struktur kromosom barley setelah diberikan perlakuan 1 mM BAPTA dibandingkan dengan kromosom kontrol. Kromosom kontrol memiliki panjang rata-rata 5,3 μm dengan struktur kromosom yang padat, sedangkan kromosom dengan perlakuan BAPTA memiliki panjang rata-rata 10,7 μm dengan struktur kromosom lebih renggang dan kurang padat. Hasil tersebut menunjukkan bahwa ion kalsium memiliki peranan penting dalam menjaga struktur kromosom barley.

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Chromosomes are genetic substances in living organisms that are passed on to the next generation. Chromosomes are formed through the process of condensation during the cell cycle. Factors that influence chromosome condensation are scaffold proteins and divalent cations, especially calcium ions (Ca2+). The role of calcium ions on chromosomes is still limited to human chromosomes and has never been reported on plant chromosomes. The purpose of this study was to determine the role of calcium ions on the structure of barley chromosomes using a fluorescence microscope and scanning electron microscope. The barley chromosome was isolated and then treated with 1 mM BAPTA as a calcium ion chelating agent and PBS as a control. According to the data obtained, there are differences in length and structure of barley chromosomes after being treated with 1 mM BAPTA compared to control chromosomes. The control chromosome has an average length of 5.3 μm with a compact chromosome structure and chromosomes with BAPTA treatment have an average length of 10.7 μm with a less condense chromosome structure. These results indicated that calcium ions have an important role on maintaining the structure of barley chromosomes."

"Critical Sociolinguistic Research Methods: Studying Language Issues that Matter is a guide on conducting concrete ethnographic and discourse analytic research projects, written by top scholars for students and researchers in social science fields. Adopting a critical perspective focusing on the role of language in the construction of social difference and social inequality, the authors walk the reader through five key moments in the life of a research project: composing research questions, designing the project, doing fieldwork, performing data analysis, and writing academic texts or otherwise engaging in conversation with different types of social actors about the project. These moments are illustrated by color-coded examples from the authors' experiences which help researchers and students follow the sequential stages of a project. Clear and highly applicable, with a detailed workbook full of practical tips and examples, this book is a great resource for graduate-level qualitative methods courses in linguistics and anthropology, as well as methods courses in the humanities and social sciences that focus on the role of language in research. It is a timely text for investigating language issues that matter to and have consequences for people's lives"

"[ABSTRAKLatar Belakang: Peran root surface conditioning terhadap keberadaan smear layer.Tujuan: Menganalisis hubungan antara penggunaan root surface conditioning (minosiklin 2,1% dan EDTA 24%) terhadap keberadaan smear layer setelah penghalusan akar gigi. Metoda: Sepuluh gigi manusia yang dicabut akibat kelainan periodontal dan dilakukan penghalusan akar. Gigi dipotong pada daerahsepertiga servikal, dan 30 spesimen yang terbentuk dibagi dalam tiga kelompok.Hasil: Tidak ada perbedaan signifikan terhadap tingkat keberadaan smear layer antara kelompok minosiklin maupun EDTA (p=0,759). Terdapat perbedaan signifikan antara kelompok minosiklin dan EDTA dengan salin sebagai kontrol (p=0,00). Kesimpulan: Ada hubungan antara penggunaan root surface conditioning terhadap keberadaan smear layer.

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ABSTRACTBackground: Role of root surface conditioning of the existence of smear layer.Objective: To analyze the smear layer on root surface conditioned withminocycline HCl 2,1% , EDTA gel 24%, after root planed.Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00).Conclusion: There was relationship of root surface conditioning treatment with smear layer.;Background: Role of root surface conditioning of the existence of smear layer.Objective: To analyze the smear layer on root surface conditioned withminocycline HCl 2,1% , EDTA gel 24%, after root planed.Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00).Conclusion: There was relationship of root surface conditioning treatment with smear layer., Background: Role of root surface conditioning of the existence of smear layer.Objective: To analyze the smear layer on root surface conditioned withminocycline HCl 2,1% , EDTA gel 24%, after root planed.Materials and methods: Ten human teeth removed due to chronic periodontitis were collected and root planed. The teeth were sectioned on 1/3 cervikal, 30 specimens were divided into three groups. Results: No significant differences of smear layer between minocycline and EDTA (p=0,759). There was significant differences of minocycline and EDTA group compare to saline (p=0,00).Conclusion: There was relationship of root surface conditioning treatment with smear layer.]"

"Vortex methods have emerged as a new class of powerful numerical techniques to analyze and compute vortex motion. This book addresses the theoretical, numerical, computational, and physical aspects of vortex methods and vortex motion. Vortex phenomena in fluid flows and the experimental, theoretical, and numerical methods used to characterize them are discussed in reviews by leading experts. Extensive photographs and sample computer graphics are provided. The development of large vortex structure in fluid flow is responsible for some of the most fascinating aspects of fluid dynamics, such as mixing, shearing, transport, and instability. Such issues arise in a variety of flow regimes, ranging from fundamental mathematical questions in laminar, transitional, and turbulent flow to sophisticated engineering settings and devices."