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"The mouse is a perfect model organism to study mammalian, and thus indirectly also human, embryology. Most scientific achievements that have had an important impact on the understanding of basic mechanisms governing embryo development in humans, originated from mouse embryology. Stem cell research, which now offers the promise of regenerative medicine, began with the isolation and culture of mouse embryonic stem cells by Martin Evans (who received the Nobel Prize in medicine in 2007 for this achievement) and Matthew Kaufman. This book provides an overview of mouse development, spanning from oocytes before fertilization to the state-of-the-art description of embryonic and adult stem cells. "

"Isolasi dan ekspansi sel punca kanker payudara secara in vitro tanpa menghilangkan sifat pluripotensi sel sangat diperlukan untuk mempelajari karakteristik sel punca kanker payudara. Penelitian ini bertujuan untuk mencari suatu kondisi kultur terbaik dengan sistem ko-kultur untuk mempertahankan sifat pluripotensi. Sel tersebut diko-kultur dengan feeder layer yang terbentuk dari Mouse Embryonic Fibroblast (MEF). Pengukuran ekspresi penanda permukaan CD44+/CD24-menggunakan metode spektrofluorometri, sedangkan pengukuran ekspresi gen SOX2 dilakukan secara kuantitatif dengan metode real time polymerase chain reaction. Pada pengukuran ekspresi gen SOX2 dilakukan normalisasi menggunakan house keeping gene PUM1 sebagai kontrol dalam. Hasil penelitian menunjukkan bahwa ekspresi CD44+/CD24-pada sel punca kanker payudara yang diko-kultur dengan MEFs menggunakan media CM dan DMEM lebih tinggi dibandingkan dengan yang tidak diko-kultur dengan MEF menggunakan CM dan DMEM. Ekspresi gen SOX2 meningkat pada sel yang diko-kultur dengan MEF di CM dan sel yang diko-kultur dengan MEF dalam DMEM yaitu berturut-turut 83,28 dan 48,33 kali dari kontrol masing masing. Hasil penelitian menunjukan bahwa kondisi kultur terbaik untuk mempertahankan pluripotensi sel punca kanker payudara adalah ko-kultur dengan MEF menggunakan CM sebagai media.

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In objective to understand the role and importance of breast cancer stem cells and the molecular and cellular events that occur during cancer development, it is essential to isolate and propagate breast cancer stem cells in long-term in vitro culture without loosing their pluripotency. This study aimed to find the best culture condition with co-cultured system to maintain the breast cancer stem cells pluripotency. The cell cultured on top of a feeder layer formed by mouse embryonic fibroblast (MEF). We observed the expression of breast stem cell markers CD44+/CD24-using spectrofluorometry and analyzed the expression of gene SOX2 using quantitative real-time polymerase chain reaction (qRT-PCR).

Fluorescence spectroscopy results showed that CD44+/CD24-expression of cancer stem cells co-cultured in CM and DMEM high glucose with MEF is higher than one cultured in CM or in DMEM high glucose without MEF only. After being normalized by using house keeping gene, PUM1, SOX2 gene expression levels in cells cultured in CM and DMEM with MEF i.e 83,28 and 48,33 times higher, respectively, compared to their control. Result showed that the best condition to maintain pluripotency of breast cancer stem cells was to co-culture the cells with MEF using CM as medium."

"This volume will cover a series of reviews on stem cells including adult and embryonic stem cells. Speakers were invited to present these talks during the Stem Cell Symposia in fall of 2010, in Samsun, Turkey. Unique aspect of this volume is that it brings a multidisciplinary aspect of stem cells extracted from a symposium."

"Neural development and stem cells, provides neuroscientists with a handy guide to stem cells in the nervous system, tracing with great clarity the development of stem cells from differentiation to neurons, astrocytes, and oligodendrocytes."

"This book describes the nuclear reprogramming, chronicling how the field has developed over the last 50-60 years."

"The main objective of this book is to provide a comprehensive review on stem cells and their role in tissue regeneration, homeostasis and therapy. In addition, the role of cancer stem cells in cancer initiation, progression and drug resistance are discussed. The cell signaling pathways and microRNA regulating stem cell self-renewal, tissue homeostasis and drug resistance are also mentioned. Overall, these reviews will provide a new understanding of the influence of stem cells in tissue regeneration, disease regulation, therapy and drug resistance in several human diseases."

"Sel induk(Stem Cell) adalah sel pembangun setiap organ dan jaringan tubtih kita. Dia adalah sel yang belum berdiferensiasi, tapi dengan kondisi tepat mampu berkembang inenjadi jaringan khusus dan organ tertentu. Sebdgian besar dari sel-sel jaringan tidak dapat beregenerasi jika rusak berat atau sakit, seperti pada kasus Infark Miokard. Penelitian terbaru mulai berfokus pada sel induk, yang dapat berproliferasi, dan berdiferensiasi menjadi sel otot jantung. Artikel ini bertujuan untuk ineiierangkan transplantasi sel induk pada infark miokard, keniajuan dan kendala yang terdapat dalam btdang kardiotnioplasti selular. (Med J Indones 2005; 15:3-8).

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Stem cells are the foundation cells for every organ and tissue in the body. They are undifferenciated cells that under proper conditions begin to develop into specialized tissues and organs. Most of the body's specialized cells cannot be replaced by natural processes if they are seriously damaged or diseased, such as in Myocardial Infarction. Recent interest has focused on stem cells, which can proliferate, and differentiate into cardiomyocytes.This paper aim to provide overview of stem cell transplantation in myocardial infarction, milestones and setbacks in the study of cellular cardiomyoplasty. (Med J Indones 2005; 15:3-8)."

"Masalah kebutaan adalah salah satu masalah kesehatan yang memprihatinkan. Kedokteran regeneratif lewat rekayasa jaringan dan optimalisasi regenerasi jaringan melalui sel induk lokal atau transplantasi sel induk telah mendapat banyak perhatian. Tinjauan pustakan kali ini membahas penemuan penting sel induk mata dan eksperimen in vivo transplantasi sel induk serta tren terkini di bidang rekayasa jaringan mata.

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AbstractBlindness is one of the most devastating condition for mankind. Regenerative medicine through tissue engineering and optimizing tissue regeneration through local adult stem cell differentiation or stem cell transplantation has received a lot of attention. This review presents highlights in the discovery of ocular stem cell and in vivo experiment for stem cell transplantation and current trends in tissue engineering of the eye. "