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"Bifidobacterium usus maternal merupakan faktor penting yang mempengaruhi kolonisasi Bifidobacterium pada usus neonatus. Kolonisasai bakteri tersebut penting pada neonatus karena dapat mempengaruhi kesehatan dan sistem imunnya di masa yang akan datang, dengan demikian faktor yang dapat mempengaruhi jumlah Bifidobacterium usus maternal penting untuk diketahui. Berbagai penelitian telah menunjukkan bahwa diet tinggi lemak dapat mempengaruhi jumlah Bifidobacterium usus. Tujuan penelitian ini adalah untuk mengetahui korelasi antara asupan lemak total dan rasio asupan asam lemak omega-6 terhadap omega-3 dengan jumlah Bifidobacterium usus maternal pada kehamilan trimester ketiga. Lima puluh dua ibu hamil (33-37 minggu) pada 10 puskesmas kecamatan Jakarta Timur ikut serta pada penelitian potong lintang ini. Asupan lemak total dan rasio asupan asam lemak secara berurutan dinilai dengan metode 2-day repeated 24 hours food recall dan SQFFQ. Jumlah Bifidobacterium usus dianalisa menggunakan quantitative real-time PCR. Penelitian ini menunjukkan bahwa terdapat korelasi positif lemah antara asupan lemak total dengan jumlah Bifidobacterium usus maternal pada kehamilan trimester ketiga. Namun belum dapat membuktikan adanya korelasi yang bermakna antara rasio asupan asam lemak omega-6 terhadap omega-3 dengan jumlah Bifidobacterium usus maternal pada kehamilan trimester ketiga.

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Bifidobacterium colonization in neonates is crucial because it has a large effect on their health and immunity in the future, thus factors that influenced the maternal gut Bifidobacterium became important to known because maternal gut Bifidobacterium is the most important factor to determined the initial colonization. Dietary intervention studies have shown that high fat diet can dramatically changed the nurmbers of gut Bifidobacterium.

The aim of this study was to examine the correlation between total dietary fat intake and the ratio of essential fatty acids with the numbers of maternal gut Bifidobacterium on third trimester of pregnancy. Fifty two pregnant women (33?37 weeks) from 10 district primary health care in East Jakarta were enrolled in this cross sectional study. Dietary fat intake and the ratio of essential fatty acids was assesed with 2-day repeated 24 hours food recall and SQFFQ, respectively. Maternal gut Bifidobacterium was analysed by quantitative real-time PCR.

This present study have shown that total dietary fat intake have a weak positive correlation with maternal gut Bifidobacterium in third trimester of pregnancy. Hormonal and immunological changes in pregnancy is the possible explanation to this phenomenon. This study have not been able to prove a correlation between omega-6 to omega-3 fatty acid ratio with maternal gut Bifidobacterium."

"Asupan asam lemak omega-3 penting selama kehamilan dan mempengaruhi luaran lahir. Sedikit data yang terbit di Indonesia mengenai asupan lemak omega-3 pada ibu hamil (bumil) dan hubungannya terhadap ukuran lahir bayi. Desain potong lintang terhadap bumil yang terdaftar ANC (antenatal care) dengan usia kehamilan >32 minggu di semua puskesmas kecamatan di Jakarta timur.Kuesioner terstruktur, semi-quantitative food frequency questionnaire (SQFFQ), shorr board, and HemoCue digunakan untuk menilai faktor ibu dan asupan omega-3 serta mengukur antropometri dan kadar hemoglobin. Pita SECA, timbang bayi dan data rekam medik digunakan untuk mengukur lingkar lengan atas ibu, berat bayi dan mencatat panjang bayi. Analisis multivariate digunakan untuk menilai hubungan asupan omega-3 terhadap ukuran lahir setelah faktor pengganggu dikendalikan. Bumil dengan asupan lemak kurang dari 54.27 mg/hr akan 2.1 (95% CI: 1.16-4.03) kali beresiko memiliki anak dengan lingkar kepala (LIKA) sub normal. Bumil dengan asupan α-linolenat kurang dari 0.82 gr/hr akan memiliki bayi dengan berat lahir 95 (95%CI:9.3-180) gram lebih rendah. Tidak ada asupan asam lemak essensial yang berhubungan dengan panjang badan. Lemak berhubungan dengan LIKA sedangkan ALA berhubungan dengan berat lahir.

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Dietary intake of omega-3 fatty acids (FAs) is important during pregnancy and influence birth outcome. Limited published study in Indonesia related to dietary omega-3 FAs intake among pregnant women and its association with infant birth size. A cross-sectional study was designed among registered antenatal care and >32 weeks of gestational age pregnant women in all sub-district public health centers in East Jakarta.

Structured interview, semi-quantitative food frequency questionnaire (SQFFQ), shorrboard, and HemoCue were used to assess maternal factors, usual intake of omega-3 FAs, measure anthropometric and hemoglobin level data respectively. Mid-upper arm circumference (MUAC) SECA tape, pediatric weighing scale and medical record were used to assess infant head circumference, birth weight and length, respectively.

Multivariate analysis was used to find adjusted association between omega-3 FAs intake and birth size. Pregnant women with fat intake below 54.27 mg/d would have 2.1 (95% CI: 1.16-4.03) times higher risk to have sub normal head circumference while pregnant women with alpa-linolenic acid (ALA) intake below 0.82 gram/d would have 95 (95%CI: 9.3-180) gram lower birth weight in adjusted data. None of omega-3 fatty acids was associated with birth length. Fat and ALA had association with head circumference and birth weight, respectively."

"Asam lemak omega-3 merupakan komponen penting pada pembentukan sel saraf, retina dan membran sel. Penelitian terakhir menunjukkan bahwa asam lemak omega-3 berkaitan dengan pertumbuhan janin. Ibu hamil dengan asupan asam lemak omega-3 yang tidak tercukupi memiliki risiko lebih tinggi untuk melahirkan bayi berat lahir rendah (BBLR). Di Indonesia, angka BBLR masih tergolong tinggi. Oleh karena itu, kecukupan asam lemak omega-3 sangat penting untuk diperhatikan pada ibu hamil sejak awal kandungan. Penelitian ini bertujuan untuk mengetahui kecukupan asam lemak omega-3 ibu hamil di Indonesia. Desain penelitian yang digunakan adalah studi potong lintang. Subjek penelitian merupakan ibu hamil sehat dengan usia gestasi hingga 20 minggu. Kecukupan dinilai melalui pendataan asupan asam lemak omega-3, omega-6 beserta rasionya dan pemeriksaan kadar asam lemak omega-3 membran eritrosit. Data penelitian kemudian dianalisis dengan uji korelasi Spearman. Hasil penelitian menunjukkan bahwa asupan asam lemak omega-3 total subjek penelitian masih kurang akibat rendahnya rerata asupan ALA subjek, meskipun rerata asupan EPA dan DHAnya telah terpenuhi. Asupan asam lemak omega-6 total subjek telah terpenuhi, begitu pula dengan asupan LAnya, tetapi asupan AA subjek masih belum terpenuhi. Rasio asupan asam lemak omega-6/omega-3 sebagian besar subjek masih kurang baik. Dari uji korelasi ditemukan adanya korelasi positif bermakna antara asupan EPA dengan kadar DHA (r=0,34) dan kadar EPA+DHA (r=0,38), asupan DHA dengan kadar DHA (r=0,31), asupan EPA+DHA dengan kadar DHA (r=0,34) dan kadar EPA+DHA (r=0,35). Rasio asupan asam lemak omega-6/omega-3 ditemukan memiliki korelasi negatif bermakna dengan kadar EPA (r= ?0,34). Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa asam lemak omega-3 ibu hamil belum tercukupi sehingga diperlukan edukasi dan screening kecukupan asupan asam lemak omega-3 bagi ibu hamil sejak awal kandungan.

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Omega-3 fatty acids are important components of neural, retinal and cell membranes. Latest findings show that omega-3 fatty acids play a role in fetal growth. Pregnant women with low intakes of omega-3 fatty acids were found to have higher risk of low birth weight (LBW). In Indonesia, LBW prevalence is still high. Thus omega-3 fatty acids sufficiency is of great importance starting early in pregnancy. The aim of this study is to determine omega-3 fatty acids status of pregnant women in Indonesia. The design of the study is cross-sectional. Subjects are healthy pregnant women with gestational age up to 20 weeks pregnancy. Omega-3 fatty acids status obtained from interview on intakes of omega-3 fatty acids and level of omega-3 fatty acids in erythrocyte membrane. The data?s then analyzed using Spearman correlation test. The study results show that total omega-3 fatty acids intakes are insufficient, due to low ALA intake, even though EPA and DHA intakes are sufficient. Intakes of total omega-6 fatty acids and LA are sufficient but AA intake is very low. The ratio of omega-6/omega-3 fatty acids intake is found to be higher than expected. Spearman correlation test shows significant positive correlations between intake of EPA and DHA level (r=0.34), intake of EPA and EPA+DHA level (r=0.38), intake of DHA and its level (r=0.31), intake of EPA+DHA and DHA level (r=0.35), intake of EPA+DHA and its level (r=0.34). Ratio of omega-6/omega-3 fatty acids intake is found to be significantly correlated with EPA level (r=?0.34). In conclusion, omega-3 fatty acids intake of pregnant women in Indonesia is still insufficient. Therefore, education to increase omega-3 fatty acids intake during pregnancy and screening of omega-3 fatty acids intake need to be perform early in pregnancy."

"Bifidobacterium merupakan salah satu mikrobiota yang memberikan manfaat bagi kesehatan manusia termasuk pada kehamilan, dan penting pada proses kolonisasi mikrobiota usus bayi baru lahir. Jumlah Bifidobacterium usus pada dewasa relatif stabil, namun belum diketahui jumlahnya pada ibu hamil trimester ketiga, terutama di Indonesia. Asupan makanan termasuk serat dapat mempengaruhi pertumbuhan Bifidobacterium, termasuk serat. Stimulasi serat terhadap pertumbuhan Bifidobacterium dapat berupa stimulasi langsung sebagai prebiotik, atau secara tidak langsung sebagai substrat yang dapat difermentasi dan menurunkan pH kolon dan meningkatkan enzim intestinal alkaline phospatase (IAP). Dua mekanisme terakhir secara tidak langsung menurunkan jumlah bakteri patogen sehingga jumlah Bifidobacterium meningkat. Penelitian potong lintang di seluruh Puskesmas Kecamatan di Jakarta Timur pada bulan Maret-Juni 2015 dilakukan untuk menilai korelasi asupan serat dengan jumlah Bifidobacterium usus ibu hamil trimester ketiga. Lima puluh dua subjek menyelesaikan prosedur penelitian. Asupan serat dinilai dengan Food Frequency Questionnaire semikuantitatif, dan kuantifikasi Bifidobacterium dengan real time PCR. Nilai asupan serat adalah 18,9 (5,6?43,0) g/hari, dan 92,3% subjek tidak memenuhi AKG. Jumlah Bifidobacterium usus adalah 7,7 (5,12?9,50) log sel/g feses. Tidak terdapat korelasi bermakna (p >0,05) antara asupan serat total, serat larut dan tidak larut, dengan jumlah Bifidobacterium usus (r = 0,223; r = 0,245; r = 0,2).

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Bifidobacterium is one of the beneficial microbiota in human health, including in pregnancy and important for first intestinal microbiota colonization in newborn. The number of intestinal Bifidobacterium in adults is relatively stable, but still unknown in the third trimester of pregnancy, especially in Indonesia. Dietary intake is one of the factors influencing the growth of Bifidobacterium, including dietary fiber. Dietary fiber stimulation act directly as a prebiotic, or indirectly as a fermentation substrate that promote the decreasing of colonic pH, and increasing intestinal alkaline phospatase (IAP) enzyme, resulting a decrease of the amount of pathogenic microbiota. A cross-sectional study in all district public health care in the East Jakarta, March until June 2015 was performed to assess the correlation between dietary fiber intake and the amount of intestinal Bifidobacterium in third trimester of pregnancy women. Fifty-two subjects completed the study procedures. Dietary fiber intake was assessed using semiquantitative Food Frequency Questionnaire, and instestinal Bifidobacterium was quantified using real time Polymerase Chain Reaction (rPCR). Dietary fiber intake in this study was 18.9 (5.6?43.0) g/day and 92.3% subjects did not meet the Dietary Reference Intake. The intestinal Bifidobacterium count is 7.7 (5.12?9.50) log cell/g faeces. The results show that there is no significant correlation (p > 0.05) between dietary fiber, dietary soluble fiber, and dietary insoluble fiber intake with the amount of intestinal Bifidobacterium in third trimester of pregnancy (r = 0.223; r = 0.245; r = 0.2)."

"Zat besi merupakan mikronutrien esensial yang diperlukan tubuh seperti pertumbuhan sel darah merah dan sel otak Kebutuhan besi meningkat pada masa kehamilan Komposisi mikrobiota dapat berubah selama tahap kehidupan yang dipengaruhi oleh berbagai faktor misalnya besi Penelitian ini merupakan penelitian dengan desain potong lintang yang bertujuan untuk mengetahui korelasi antara asupan besi dan kadar feritin serum dengan jumlah Bifidobacterium pada ibu hamil trimester ketiga Penelitian ini dilakukan di seluruh puskesmas kecamatan di Jakarta Timur dari bulan Maret sampai April 2015 Pengambilan subjek dilakukan dengan cara konsekutif dan didapatkan 52 ibu hamil yang memenuhi kriteria penelitian Data dikumpulkan dengan wawancara meliputi kuesioner data asupan besi heme dan non heme protein serta vitamin C dengan FFQ semikuantitatif Pengukuran antropometri untuk menilai status asupan gizi pemeriksaan laboratorium untuk mengetahui kadar feritin serum dan kadar CRP serta jumlah Bifdobacterium dalam tinja Didapatkan rerata usia 29 1 5 9 tahun nilai median asupan besi 66 7 3 3 144 1 mg hari kadar feritin serum 31 1 3 6 96 1 g L dan jumlah Bifidobacterium usus 7 45 5 1 9 5 log g tinja Tidak didapatkan korelasi yang bermakna asupan besi dengan jumlah Bifidobacterium usus r 0 202 p 0 152 juga tidak didapatkan korelasi bermakna antara kadar feritin serum dengan jumlah Bifidobacterium usus (r=0,116 p=0,411).

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Iron is an essensial micronutrient which body needed such as for blood growth cell and brain cell Iron rsquo s requirement increases in pregnancy Microbiota composition can change in cycle of life which be affected by many factors likely iron The aim of this cross sectional study was to find the correlation between iron intake and serum ferritin with Bifidobacterium third trimester of pregnancy Data collection was conducted from March 2015 until April 2015 in all of sub district of public health centre in east Jakarta Subjects were obtained using consecutive sampling method A total of 52 pregnancy subjects had met the study criteria Data were collected through interviews including questionnare iron intake heme and non heme protein and vitamin C used semiquantitative FFQ Anthropometry measurementsto assess the nutritional status and laboratory examination i e blood levels of serum ferritin and CRP and Bifidobacterium in feces Mean age 29 1 5 9 years Median of intake of iron was 66 7 3 3 144 1 mg day serum ferritin was 31 1 3 6 96 1 g L and gut Bifidobacterium 7 45 5 1 9 5 log g feces No significant correlation was found between iron intake and Bifidobacterium in feces r 0 202 p 0 152 and negative correlations and no significant between serum ferritin and Bifidobacterium in feces (r=0, 116, p=0,411)"

"Latar belakang: Pada trimester ketiga terjadi peningkatan patogen yang dapat disebabkan oleh gangguan kerja imunitas usus akibat defisiensi seng, sehingga menekan pertumbuhan Bifidobacterium. Penelitian ini bertujuan untuk mengetahui korelasi antara kadar seng serum dan jumlah Bifidobacterium usus pada kehamilan trimester ketiga. Desain: Studi potong lintang pada 52 wanita hamil ≥32 minggu, berusia 19–44 tahun dan memenuhi kriteria penelitian di 10 Puskesmas Kacamatan Jakarta Timur. Dinilai asupan seng dan besi menggunakan SQ-FFQ. Asupan protein, lemak dan total kalori menggunakan 2x24 hours food recall. Dilakukan pengukuran seng serum dan penghitungan Bifidobacterium usus. Hasil: Didapatkan asupan seng kurang dengan rerata 8,74±3,90 mg/hari. Defisiensi seng didapatkan pada 75% subjek. Jumlah Bifidobacterium usus subjek memiliki median sebesar 7,7 (5,12–9,50) log sel/gram. Kelompok defisiensi seng memiliki nilai median yang lebih rendah. Uji korelasi didapatkan nilai r=0,04 dengan p=0,81. Kesimpulan: Tidak ditemukan korelasi antara kadar seng serum dan jumlah Bifidobacterium usus kehamilan trimester ketiga.

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Background: Numbers of pathogen were increases in the third trimester of pregnancy that can be caused by impairment of gut immune function due to zinc serum deficiency, thereby suppressing the growth of Bifidobacterium. This study was conducted to investigate the relationship between zinc serum levels and gut Bifidobacterium numbers in the third trimester of pregnancy.

Design: A cross-sectional study recruited 52 pregnant women among 19–44 years old with gestational age ≥32 weeks and met the study criteria were conducted in 10 Community Health Center at East Jakarta. Dietary intake such as zinc and iron through SQ-FFQ, protein, fat and total calories using 2x24 hours food recall were assessed. Measurement of serum zinc level and quantification of gut Bifidobacterium numbers were generated.

Results: The entire subject had poor zinc intake with mean value 8,74±3,90 mg/day. Zinc deficiency was found in 75% subjects. Median number of gut Bifidobacterium was 7,7 (5,12–9,50) log cell/gram and subjects with zinc deficiency had lower median value. Correlation test score r=0,04 and p=0,81.

Conclusion: There was no correlation between serum zinc levels and gut Bifidobacterium numbers in the third trimester of pregnancy."

"Kolonisasi Bifidobacterium merupakan bakteri komensal yang baik untuk perkembangan dan kolonisasi awal mikrobiota janin. Jumlah Bifidobacterium dapat dipengaruhi oleh asupan protein ibu selama hamil. Penelitian potong lintang ini dilakukan di seluruh puskesmas kecamatan di Jakarta Timur mulai bulan Februari hingga April 2015 dengan subjek ibu hamil berusia 19 - 44 tahun dan usia kehamilan 32 - 37 minggu. Data asupan protein didapatkan dengan metode 2-day repeated 24 hour food recall, selain itu dinilai juga rasio asupan nabati- hewani menggunakan metode semi quantitative - food frequency questionnaire (SQ-FFQ). Analisis feses dilakukan pada 52 subjek menggunakan metode real time-polymerase chain reaction (rPCR). Hasil penelitian ini memperlihatkan terdapat korelasi positif lemah tidak bermakna antara asupan protein dengan jumlah Bifidobacterium (r = 0,132, p >0,05), sehingga penelitian ini belum dapat membuktikan adanya korelasi antara asupan protein dengan jumlah Bifidobacterium pada ibu hamil trimester ketiga.

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Bifidobacterium is a commensal bacteria that are beneficial for the development and early colonization of microbiota on fetus. The amount of Bifidobacterium can be influenced by maternal protein intake during pregnancy. A cross-sectional study had been conducted in all primary health care in East Jakarta Subdistrict, from February to April 2015. Subjects of the study were pregnant women aged 19-44 years old and gestational age 32-37 weeks. The quantity of protein intake was obtained by 2-day repeated 24 hour food recall method, moreover, the study also assessed the intake of vegetable-animal ratio by semiquantitative-food frequency questionnaire (SQ-FFQ) method. Stool analysis was conducted on 52 subjects using real-time polymerase chain reaction (rPCR). The result of the study showed a poor positive correlation between protein intake with the amount of Bifidobacterium (R = 0.132, p >0.05).This study has not showed any significant correlation between protein intake with the amount of Bifidobacterium in the third trimester of pregnancy."

"Penelitian ini merupakan penelitian potong lintang di Departemen Radioterapi Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo Jakarta, yang bertujuan untuk mengetahui korelasi antara kadar asam lemak omega-3 terhadap massa otot dan kekuatan genggam pada subjek kanker kepala leher yang mendapatkan radioterapi. Kaheksia kanker sering terjadi pada kanker kepala leher akibat peningkatan sitokin proinflamasi yang menyebabkan hipermetabolisme, peningkatan kebutuhan nutrisi, anoreksia, penurunan massa otot dan berat badan. Asam lemak omega-3 berperan dalam menurunkan inflamasi, meningkatkan massa otot, dan kekuatan genggam. Dari 52 subjek yang sudah mendapatkan radioterapi ge;25 kali, 57 adalah laki-laki dengan rerata usia di atas 50 tahun. Lokasi kanker paling banyak di area nasofaring, sebagian besar sudah berada pada stadium IV dan mendapatkan kombinasi radioterapi dan kemoterapi. Sebesar 38,5 dan 32,7 subjek berada pada kategori indeks massa tubuh normal dan kurang. Data yang diperoleh dari penelitian ini dapat memberikan gambaran kurangnya asupan energi, protein, lemak, dan asam lemak omega-3, serta massa otot sebagian besar subjek yang tergolong kecil 28,4 4,7 , dengan kekuatan genggam sebagian besar subjek tergolong normal, dan kadar asam lemak omega-3 plasma seluruh subjek yang tergolong rendah 2,5 0,8 . Data tersebut menunjukkan adanya masalah nutrisi pada pasien kanker kepala leher. Terdapat korelasi yang kuat antara kadar asam lemak omega-3 plasma terhadap massa otot r =0,6, p 50?60 Gy dan 50 Gy.

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This cross sectional study conducted in the Department of Radiotherapy Dr. Cipto Mangunkusumo Hospital, aimed to investigate the correlation between omega 3 fatty acids plasma levels with muscle mass and hand grip muscle strength in subjects with head and neck cancer undergoing radiotherapy. Cancer cachexia is common in head and neck cancer as a result of the increasing of proinflammatory cytokines that cause hipermetabolisme, increased nutritional needs, anorexia, decreased muscle mass and body weight. Omega 3 fatty acids play a role in reducing inflammation, as well as improving muscle mass and hand grip. There were 52 subjects who had received radiotherapy ge 25 times, 57 were male with a mean age of 50 years. Most cancer sites were at nasopharynx area, mostly in stage IV and received a combination of radiotherapy and chemotherapy. There were 38,5 of the subjects in the normal body mass index and 32,7 were in low body mass index. The data from this study showed inadequate intake of energy, protein, fat, and omega 3 fatty acids, as well as muscle mass majority was small 28,4 4,7 , with most of the hand grip classified as normal, and the plasma levels of omega 3 fatty acids all of the subjects were low 2,5 0,8 . The data showed that there were nutritional problems in patients with head and neck cancer. There was strong correlation of plasma levels of omega 3 fatty acids with muscle mass r 0,8, p 50 60 Gy and 50 Gy. "

"Status gizi pada ibu hamil mempengaruhi komposisi mikrobiota usus ibu yang secara tidak langsung akan mempengaruhi pembentukan mikrobiota usus anak. Penelitian ini adalah suatu studi potong lintang yang mencari korelasi peningkatan berat badan dan lingkar lengan atas dengan jumlah Bifidobacterium dan Lactobacillus pada 52 ibu hamil trimester ketiga. Penelitian dilaksanakan di 10 Puskesmas Kecamatan Jakarta Timur selama bulan Februari?April 2015. Uji korelasi peningkatan BB dengan jumlah Bifidobacterium (r = 0,119, p = 0,4) dan dengan jumlah Lactobacillus (r = -0,009, p = 0,951). Korelasi LLA dengan jumlah Bifidobacterium (r = -0,211, p = 0,134) dan dengan jumlah Lactobacillus (r = - 0,013, p = 0,926). Dengan demikian, penelitian ini belum dapat membuktikan bahwa terdapat adanya korelasi antara peningkatan BB dan LLA dengan jumlah Bifidobacterium dan Lactobacillus pada kehamilan trimester ketiga.

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Maternal nutritional status influences maternal gut microbiota composition, which in turn shapes the infant?s gut microbiota composition. Recent studies have shown that gut microbiota regulates obesity by increasing energy harvest from diet and by regulating peripheral metabolism. This cross-sectional study reports the correlation of maternal weight gain and mid-upper arm circumference with Bifidobacterium and Lactobacillus on 52 third-trimester pregnant women. The study was done on February?April 2015 in 10 Primary Health Care Centres in East Jakarta. Correlation of maternal weight gain with Bifidobacterium (r = 0.119, p = 0.4) and with Lactobacillus (r = -0.009, p = 0.951). The correlation of MUAC with Bifidobacterium (r = -0.211, p = 0.134) and Lactobacillus (r = -0.013, p = 0.926). Thus, this study has not proven any correlation between maternal weight gain and MUAC with Bifidobacterium and Lactobacillus count."

"ABSTRAKBifidobacterium, mikrobiota yang bermanfaat terhadap kesehatan, jumlahnya dipengaruhi oleh diet, genetik, dan usia. Makanan khas Minangkabau dan Sunda yang mengandung tinggi lemak, dan Healthy Eating Index HEI yang berhubungan kuat dengan asupan asam lemak jenuh dapat digunakan untuk mengukur kualitas diet. Penelitian potong lintang pada wanita 19-50 tahun dilakukan untuk menilai hubungan HEI dan asupan asam lemak jenuh dengan jumlah Bifidobacterium usus pada wanita keturunan Minangkabau dan Sunda. Data asupan diperoleh menggunakan 2-day repeated 24-hour food recall, pemeriksaan jumlah Bifidobacterium menggunakan real time quantitative Polymerase Chain Reaction. Tidak terdapat hubungan antara HEI dengan jumlah Bifidobacterium, namun cenderung terdapat hubungan antara asupan asam lemak jenuh dengan jumlah Bifidobacterium pada wanita keturunan Minangkabau dan Sunda setelah dikontrol dengan faktor pengganggu.

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ABSTRACTBifidobacterium, the beneficial microbiota on human health, is influenced by various factors such as dietary intake. Minangkabau and Sundanese food are contain of high fat, and Healthy Eating Index HEI that is strongly associated with saturated fatty acid intake can be used to measure the quality of diet. A cross sectional study of women aged 19 50 years was conducted to assess the relationship between HEI and saturated fatty acids intake with intestinal Bifidobacterium in Minangkabau and Sundanese women. Dietary intake was assessed by 2 day repeated 24 hour food recall and Bifidobacterium was quantified using real time quantitative Polymerase Chain Reaction. There was no significant relationship between HEI with intestinal Bifidobacterium, but saturated fatty acids intake tend to have association with intestinal Bifidobacterium in Minangkabau and Sundanese women after adjustment for confounding factors."