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"This book covers the methods and applications of bioassays in pharmaceutical science to quantify drug activity and evaluate the validity of models. It demonstrates the numerous systems available and that each system interprets the activity of drug molecules in different ways. The authors discuss the various models put forward to link the experimental observations and the events taking place on a cell or molecular level between the drug and experimental object, providing the principal and technological guide for successful research of drug research and clinical therapy."

"This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine.In this thesis, the author firstly describes the need and concept of partipetting for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. "

"This book, the first comprehensive, integrated treatment of the subject, Assay development : fundamentals and practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. After discussing the basics, it explains the treatment of assays for purified protein, cell-based assays, and high-throughput screening. It includes good practices and case studies. "

"Ikan cakalang merupakan salah satu sumberdaya ikan pelagis bernilai ekonomis yang didaratkan di PPN Palabuhanratu. Penelitian bertujuan mengetahui aspek biologi, status keberlanjutan pengelolaan, dan menyusun model dan strategi pengembangan pengelolaan secara berkelanjutan. Metode pengambilan sampel ikan cakalang secara acak terhadap kapal pancing tonda yang mendarat di PPN Palabuhanratu. Sedangkan untuk analisis status keberlanjutan menggunakan Rapid Appraisal for Fisheries (RAPFISH), dan untuk menyusun prioritas strategi menggunakan metode Proses Hirarki Analitik (AHP). Aspek biologi menunjukkan pola pertumbuhan bersifat allometrik negatif. Nisbah kelamin menunjukkan bahwa ikan betina lebih banyak dari pada ikan jantan. Ikan cakalang yang tertangkap kebanyakan pada TKG III sebesar 42%, dimana telah mengalami pemijahan. Hasil analisis potensial lestari (MSY) sebesar 741.039 kg/tahun, fmsy = 1.392 unit dan CPUEopt adalah 532.050 kg/unit; Status keberlanjutan pengelolaan perikanan tangkap ikan cakalang di PPN Palabuhanratu secara multidimensi dalam kondisi kurang berkelanjutan (nilai indeks 46,19). Strategi yang perlu dilakukan berdasarkan skala prioritas adalah 1) Pengaturan alat bantu penangkapan ikan, 2) Penentuan selektivitas alat penangkapan ikan, 3) Pembatasan upaya penangkapan ikan, 4) Peningkatan kualitas SDM, 5) Pengaturan perubahan target tangkapan sementara sesuai musim, 6) Pengelolaan hasil tangkapan sampingan (by catch), 7) Peningkatan pengawasan penangkapan ikan, 8) Pengembangan alat penangkapan ikan yang ramah lingkungan, 9) Meningkatkan nilai GDP, 10) Pengaturan kepemilikan sumberdaya, 11) Kebijakan dalam pengelolaan perikanan mempertimbangkan kearifan lokal nelayan setempat, 12) Meningkatkan Peran Sektor Perikanan, dan 13) Status konflik.

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Skipjack tuna is one of the major pelagic fish resources which has economic value that landed on PPN Palabuhanratu. The research aims to determine the biological aspects, determine the status management of sustainability, and model and strategy of sustainability development management. Sampling methods is randomly collect the skipjacks tuna on the trolling ship that landed in PPN Palabuanratu. Moreover, in the purpose of analysis of sustainability status is using Rapid Appraisal for Fisheries (RAPFISH), to arrange the strategic priorities is using Analytical Hierarchy Process (AHP). Biological aspects show allometric growth pattern is negative. Sex ratio showed that female fish are more than the male fish. Tuna were caught mostly on TKG III by 42%, which has undergone spawning. The results of the analysis of the sustainable potential (MSY) is 741.039 kg/year, fmsy = 1.392 units and CPUEopt is 532.050 kg/unit. The status of sustainability management in multidimension point of view, skipjack tuna fisheries activities in PPN Palabuhanratu is less sustainable condition (index value of 46.19). The strategies that need to do based on priority scale are: 1) setting up the fishing tools, 2) Determination of the selectivity of fishing tools, 3) Restriction of fishing activities, 4) improving the quality of human resources, 5) Settip up the changes of temporary target catch according to season, 6) Management of side catches value (by catch), 7) Improving monitoring of fishing, 8) Developing of fishing tools that are environmentally friendly, 9) Increase the value of GDP, 10) Admission of resource ownership, 11) Policy in fisheries management that considering the value of local fishermes, 12) Increase the Role of Fisheries Sector, and 13) Status of conflict."

"ABSTRAKGelatin merupakan salah satu jenis protein yang diperoleh dari kolagen alami yang terdapat dalam kulit dan tulang hewan. Gelatin yang paling banyak digunakan ialah gelatin babi. Penggunaan gelatin dengan bahan baku hewan babi pada produk makanan dan farmasi seringkali menyebabkan alergi, selain juga tidak diperbolehkan dalam agama islam, sehingga perlu dilakukan analisis kandungan didalam makanan olahan agar aman untuk dikonsumsi. Salah satu analisis yang dapat digunakan ialah metode imunosensor, salah satunya Enzyme-Linked Immunosorbent Assay ELISA yang memiliki tingkat sensitifias yang tinggi, mampu menguji sampel yang tidak murni, dan mampu mengikat secara selektif antigen yang dikehendaki. Metode ini memerlukan antibodi anti-gelatin babi untuk mendeteksi gelatin babi sehingga dilakukan produksi antibodi poliklonal antigen gelatin babi menggunakan hewan uji kelinci. Pada penelitian ini produksi antibodi poliklonal gelatin babi dilakukan dari antigen gelatin babi dengan mengekstraksi gelatin dari kulit babi. Rendemen yang dihasilkan sebesar 20,52 dan dikarakterisasi dengan spektrofotometer UV-Vis dan FTIR serta dianalisis dengan SDS PAGE dihasilkan bahwa gelatin babi memiliki 5 pita pemisahan dan gelatin dapat dijadikan sebagai antigen. Antibodi poliklonal antigen gelatin babi dapat diproduksi dengan hewan uji kelinci dan antibodi yang diperoleh pada hari ke 65 dilakukan pemurnian IgG. Antibodi yang dipurifikasi tersebut dapat dijadikan sebagai konjugat sensor pada imunosensor berbasis ELISA dengan konsentrasi IgG sebesar 0,289 mg/mL.

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ABSTRACTGelatin is one type of protein derived from natural collagen found in the skin and animal bones. The most widely used gelatin is pork gelatin. The use of gelatin with raw materials of pigs on food and pharmaceutical products often causes allergies, as well as not allowed in Islam, so it is necessary to analyze the content in processed foods to be safe for consumption. One of the analysis that can be used is immunosensor method, one of them is Enzyme Linked Immunosorbent Assay ELISA which has high sensitivity level, able to test impure samples, and able to selectively bind antigen desired. This method requires a pig anti gelatin antibody to detect pig gelatin so that the production of polyclonal antibody of pig gelatin antigen using rabbit test animals. In this study the production of polyclonal antigen pig gelatin carried out from pig gelatin antigen by extracting gelatin from pig skin. The yield of gelatin 20.52 and characterized by UV Vis and FTIR spectrophotometers and analyzed by SDS PAGE was found that pig gelatin has 5 separation bands and gelatin can be used as antigen. Polyclonal antibodies of antigen porcine gelatin can be produced with rabbit test animals and antibodies obtained on the 65th day of IgG purification. The purified antibodies can be used as conjugate sensors on immunosensors based ELISA with a concentration of IgG of 0.289 mg mL."

"Quality assurance is necessary to maintain quality and services in the pharmaceutical and life science industries. Quality assurance demonstrates that the logic and practice of problem solving can integrate both program efficacy and regulatory compliance. This title is divided into three parts; the first part discusses the process by which a problem in regulated industry is identified, for example a manufacturing deviation that leads to an adulterated drug product, and reviews the decision-making steps involved in remedying the problem. The second part delves into the staff training requirements of procedures that are thereby revised. The third part expands on this discussion by considering piloting the proposed training module, preparing assessments of trainee proficiency, evaluating the training module, including integrating rigorous evaluative designs with formative program improvement, and documenting the entire effort. Presents a comprehensive view of the field of quality assuranceAn approach grounded in direct experienceUses diagrams and figures to clarify analytical points."

"Wound healing is a biological response that occurs following a trauma or pathologic condition of the oral mucosa. Wounds require proper management so they may heal faster and without complication. The number of fibroblast cells seen in the oral mucosa is a strong indicator of wound healing. In the remodelling phase of wound healing, fibroblast cell proliferation decreases as collagen fibres are synthesized. Noni leaf (Morinda citrifolia L.), a part of the noni plant, is traditionally used to heal soft tissue wounds in Indonesia. The leaves have potential chemical compounds that may be useful in the wound repair process. Objective: This paper aims to examine the effect of ethanol based pastes of the Indonesian noni leaf on oral mucosa wound healing by investigating visual wound closure and fibroblast cell counts in Wistar rats. Methods: Rats were divided into 2 control groups and 4 treatment groups. The pastes were formulated in concentrations of 2.5%, 5%, 10%, and 20% and were applied onto wounds of the oral mucosa of the rats. One control group was given no medication and the other control group had an ethanol gel with a concentration of 10% noni leaf extract applied to their intraoral wounds. Result: Result revealed that all groups treated with noni leaf paste experienced better wound closure (p<0.05) when compared to the control groups. Fibroblast cell counts showed little significance amongst all groups (p=0.143), however fibroblast cell counts of groups treated with noni leaf paste, of all concentrations, were lower than both control groups. Conclusion: These results suggest that topical application of noni leaf paste promotes better oral mucosa wound healing than gel formulas but shows no significant difference in the amounts of fibroblasts during the remodelling phase."