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"Magnetic Resonance Spectroscopy (MRS) membantu ahli radiologi untuk mengetahui apakah pasien mengalami glioma otak atau infeksi otak. Dalam tugas akhir ini dibahas proses klasifikasi terhadap data hasil MRS untuk mengetahui apakah pasien mengalami glioma otak atau infeksi otak. Metode yang digunakan untuk klasifikasi adalah metode AdaBoost dengan base learner K-Nearest Neighbor dan metode K-Nearest Neighbor. Hasil Percobaan yang dilakukan menunjukkan bahwa metode AdaBoost dengan base learner K-Nearest Neighbor dengan K=3 mempunyai nilai akurasi 97% pada data training 80% sementara nilai akurasi dari metode K-Nearest Neighbor 94.4 % pada data training 80%. Hasil akhir dari pembuatan tugas akhir ini adalah sebuah perangkat lunak pendukung keputusan ( Decision Support System) yang membantu memberikan informasi apakah pasien mengalami glioma otak atau infeksi otak.

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Magnetic Resonance Spectroscopy (MRS) helps the experts of radiology to detect the brain glioma or brain infection in patients. In this final project, the classification process on the result of MRS data is discussed to detect the brain glioma or brain infection in patients. The used classification methods are AdaBoost with base learner K-Nearest Neighbor and K-Nearest Neighbor methods. The result of research shows that the AdaBoost method with base learner K-Nearest Neighbor with K=3 has 97% accuracy value on 80% training data, while the accuracy value from K-Nearest Neighbor method is 94.4 % on 80%training data. The result from the writing of this final project is the software for making decision (Decision Support System) that supports the giving of information on the existence of brain glioma or brain infection in patients."

"[Ilmu Kedokteran Gigi merupakan salah satu bidang yang rawan untukterjadinya kontaminasi silang antara pasien-dokter gigi, pasien-pasien dan pasien perawat. Kontrol Infeksi dapat mencegah terjadinya infeksi silang. Tujuan: Melihat hubungan Pengetahuan, Perilaku dan Faktor Lingkungan terhadap keberhasilan upaya Kontrol infeksi pada Mahasiswa Program Profesi Fakultas Kedokteran gigi Univesritas“x” di Jakarta. Metode: Metode penelitian yang digunakan cross sectional dengan Model penelitian Sequential Explonatory design. Penelitian dilakukan pada Mahasiswa Program Profesi Fakultas Kedokteran Gigi Universitas “X” di Jakarta yang sudah melewatipendidikan profesi selama 2 (dua) semester, dengan jumlah sampel sebanyak 101 mahasiswa. Hasil: Upaya Kontrol Infeksi pada Mahasiswa Program Profesi Fakultas Kedokteran gigi universitas “X” di Jakarta masih kurang baik.Ini dipengaruhi oleh pengetahuan, perilaku dan faktor lingkungan masih kurang baik dan mempunyai p value< 0,05 sehingga mempunyai hubungan yang signifikan terhadap upaya kontrol infeksi. Kesimpulan: Pengetahuan dan Perilaku dapat ditingkatkan dengan membentuk program yang berisikan promosi kesehatan dalam bentuk poster-poster, standart operasional prosedur berdasarkan standard precautions dan seminar-seminar bagimahasiswa program profesi fakultas kedokteran gigi;Background: Dentistry is one of a science which has a high sensitivity to causedcross-contamination between the patient-dentist, patient-patient and patient-nurse. Infection control may prevent cross-infection. Objective: Knowing the relationship between Knowledge, Behavior and Environmental Factors with successfull of Infection Control by the students of Professional Program Study of the Faculty of Dentistry, Universitas "x" in Jakarta. Methods: The method used is cross sectional with research model Sequential Explonatory Design. The study which has the total sample of 101 students was conducted and involved thestudents of Professional Program Study of the Faculty of Dentistry, University "X" in Jakarta, which has passed the professional education for two (2) semesters. Results: Infection Control Efforts conducted by the student of Professional Program Study Faculty of Dentistry, University "X" in Jakarta is not maximal. It was affected by the Knowledge, Behavior and Environmental Factors which not good enough and has a p value <0.05 and therefore has a significant relationship which infection control effort. Conclusion: Knowledge and Behavior can be improved by establishing a program containing health promotion in the form of posters, standard operating procedures based on standard precautions and seminars for the students of Professional Program Study of the faculty of dentistry., Background: Dentistry is one of a science which has a high sensitivity to causedcross-contamination between the patient-dentist, patient-patient and patient-nurse.Infection control may prevent cross-infection. Objective: Knowing therelationship between Knowledge, Behavior and Environmental Factors withsuccessfull of Infection Control by the students of Professional Program Study ofthe Faculty of Dentistry, Universitas "x" in Jakarta. Methods: The method used iscross sectional with research model Sequential Explonatory Design. The studywhich has the total sample of 101 students was conducted and involved thestudents of Professional Program Study of the Faculty of Dentistry, University"X" in Jakarta, which has passed the professional education for two (2) semesters.Results: Infection Control Efforts conducted by the student of ProfessionalProgram Study Faculty of Dentistry, University "X" in Jakarta is not maximal. Itwas affected by the Knowledge, Behavior and Environmental Factors which notgood enough and has a p value <0.05 and therefore has a significant relationshipwhich infection control effort. Conclusion: Knowledge and Behavior can beimproved by establishing a program containing health promotion in the form ofposters, standard operating procedures based on standard precautions andseminars for the students of Professional Program Study of the faculty ofdentistry.]"

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Pada pagi hari yang berbahagia ini perkenankanlah saya memanjatkan puji svukur ke hadirat Allah SWT yang telah melimpahkan rahmat dan karuniaNya kepada kita semua sehingga upacara pengukuhan saya sebagai Guru Besar Tetap di Universitas Indonesia dapat diselenggarakan.

Perkembangan sosial-ekonomi dalam era globalisasi ini telah menambah kompleksnya masalah kesehatan di Indonesia. Pada saat penyakit infeksi masih belum dapat dikendalikan dengan baik. penyakit non infeksi seperti penyakit degeneratif. keganasan jantung dan sebagainya mulai meningkat sehingga terjadi beban ganda.

Pada saat ini kita berada dalam era peralihan pola penyakit. Pada bulan Januari yang lalu Badan Litbang Kesehatan RI. mengadakan diskusi ilmiah mengenai Emerging Infectious Diseases; yaitu penyakit infeksi baru, yang timbul kembali atau resister terhadap obat yang insidensnya telah meningkat dalam 20 tahun terakhir atau berpotensi untuk meningkat di masa yang akan datang.

Apa yang sekiranya dapat dianggap sebagai penyebab fenomena ini ? Perilaku masyarakat modern dengan gaya hidup baru dapat menjadi salah satu penyebabnya. Pertumbuhan penduduk yang tinggi dan terjadinya urbanisasi yang tidak diimbangi dengan sarana dan prasarana, telah menambah luasnya daerah kumuh di perkotaan. Perkembangan teknologi yang cepat dan tuntutan kebutuhan manusia yang meningkat dapat menyebabkan terjadinya perubahan lingkungan sehingga timbul masalah kesehatan lingkungan yang erat hubungannya dengan penyakit infeksi. Kurangnya air bersih, pencernaran tanah dan air oleh limbah industri dan limbah rumah tangga termasuk tinja, menciptakan kondisi lingkungan fisik yang memungkinkan perkembangan vektor dan sumber infeksi. Fenomena tersebut berlaku juga untuk salah satu kelompok penyakit infeksi yaitu penyakit parasitik.

Hadirin yang terhormat,

Sesuai dengan keahlian dan tugas saya sebagai staf pengajar di Fakultas Kedokteran Universitas Indonesia, pada kesempatan ini saya memilih judul pidato pengukuhan :

"Beberapa Infeksi Parasitik Masa Lampau dan Masa Kini di Indonesia"

Pertama, saya ingin menyampaikan mengenai sekelompok penyakit parasit yang sudah lama bercokol di masyarakat, dan masih tetap menjadi bahan pembicaraan yang muncul di berbagai media masa dan masih tetap menjadi masalah hangat yaitu :

Infeksi cacing usus yang ditularkan melalui tanah

Infeksi casing usus yang dimaksud di atas adalah infeksi yang disebabkan oleh cacing yang ditularkan melalui tanah, telur dan larvanya menjadi bentuk infektif di tanah. Dalam bahasa sehari-hari infeksi ini disebut cacingan. Infeksi dengan cacing usus yaitu Ascaris lumbricoides, Trichuris trichiura dan cacing tambang (Necator americanus dan Ancylostoma duodenale) masih merupakan masalah kesehatan masyarakat di daerah tropik, termasuk Indonesia.

Cacingan merupakan penyakit rakyat yang sangat erat kaitannya dengan masalah lingkungan. Lingkungan yang kumuh merupakan tempat subur untuk berkembang biaknya cacing-cacing penyebab cacingan.

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"The study of the patterns of nematode infection on rodents in Lore Lindu,Central Sulawesi was carried out. A total of 52 rodents were examined...."

"Recurrent abdominal pain (RAP) is a very common presenting complaint in pediatric population. There is still a debate regarding the role of Helicobacter pylori (H. pylori) infection as an etiology of RAR Typically the inflammatory process in the gastric mucosa of infected individuals is chronic gastritis. Serologic and histologic examination are widely used for the diagnosis. This study was aimed to determine the role of H.pylori infection in Indonesian children with RAR The presence of serum IgG antibody to H. pylori and upper gastrointestinal endoscopy were performed on the 101 children with RAR Mztcosal biopsies were obtained for histologic analysis. The prevalence of H. pylori infection indicated by serology was 32.7% and by histology was 27. 7%. Histologic evidence of gastritis was present in 94.1 % children and 45% of them had chronic atrophic and active gastritis. Seventy percent children with H. pylori positive were found abnormal through endoscopy and all of the infected children were revealed abnormal through histological examination. Forty eight percent of seropositive children were found H. pylori positive and 80% of seronegatives children were found to be H pylori negative through histologic examination. Conclusion: H. pylori infection can be a cause of RAP in children. Work up for H. pylori infection should be performed when symptoms are suggestive of organic disease. Larger prospective studies are needed to be perforated for a longer time of period to clarify this issue."

"ABSTRACTTalasemia adalah penyakit yang disebabkan oleh adanya kelainan dalam hemoglobin. Penyakit talasemia merupakan penyakit herediter atau penyakit keturunan dimana pembawa gen talasemia adalah orang tua dari penderita. Di Indonesia, pada tahun 2015 diketahui jumlah kasus talasemia mencapai 7.029 kasus. Sampai saat ini talasemia belum dapat disembuhkan namun dapat dikenali sifat pembawanya dengan skrining. Dalam tugas akhir ini, akan dibandingkan performa dari dua metode yang digunakan untuk mengklasifikasikan data talasemia, yaitu K-Nearest Neighbor dan Naive Bayes. Data yang digunakan adalah 82 data pasien talasemia dan 68 data pasien non-talasemia dari Rumah Sakit Anak dan Bunda Harapan Kita, Jakarta Barat. Hasil akhir menunjukkan bahwa metode Naive Bayes memberikan nilai akurasi yang lebih besar dari K-Nearest Neighbor dalam mengklasifikasikan talasemia. Rata-rata akurasi Naive Bayes sebesar 99.775% dengan rata-rata waktu running 0.0554 detik dan rata-rata akurasi K-Nearest Neighbor adalah 97.142% dengan rata-rata waktu running 0.081 detik. Untuk nilai spesifikasi, keduanya memberikan performa yang sama, yaitu dari K-Nearest Neighbor diperoleh ketika K=3 yaitu sebesar 100% dan dari Naive Bayes sebesar 100%. Hasil rata-rata sensitivitas tertingi diberikan oleh Naive Bayes yaitu sebesar 99.59%, sedangkan K-Nearest Neighbor sebesar 96.25% untuk K=1.

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ABSTRACTThalassemia is a disease caused by abnormalities in the hemoglobin. Thalassemia is a hereditary disease which the thalassemia gene carriers are parents of sufferers. In Indonesia, in 2015 it was found that the number of thalassemia cases reached 7,029 cases. Until now thalassemia has not been cured, but it can be recognized the nature of its carrier by screening. In this final project, the performance of the two methods will be compared to classify thalassemia data, namely K-Nearest Neighbor and Naive Bayes. The data used were 82 data on thalassemia patients and 68 data on non-thalassemia patients from Harapan Kita Children and Womans Hospital, West Jakarta. The final results show that the Naive Bayes method provides greater accuracy value than K-Nearest Neighbor in classifying thalassemia. The average accuracy of Naive Bayes is 99.775% with an average running time of 0.0554 seconds and the average accuracy of K-Nearest Neighbor is 97.142% with an average running time of 0.081 seconds. For specification values, both give the same performance. The result of specification values using K-Nearest Neighbor yield when K = 3 that is 100% and from Naive Bayes that is 100%. The highest average sensitivity results are given by Naive Bayes is 99.59%, while K-Nearest Neighbor is 96.25% for K = 1."

"Virus dengue (DENV) adalah penyebab penyakit infeksi yang endemik di 100 negara di dunia. DENV dapat menyebabkan infeksi primer dan sekunder. Infeksi sekunder diperkirakan lebih berat dan akan menyebabkan menjadi Demam Berdarah Dengue (DBD) dan Sindrom Renjatan Dengue (SRD). Penatalaksanaan yang lebih dini dan tepat akan membantu mengurangi terjadinya kasus berat seperti DBD dan SRD. Teknik diagnostik yang dikembangkan belum ada yang dapat mendeteksi secara cepat dan tepat pada awal infeksi terutama untuk virus dengue strain Indonesia. Tujuan penelitian ini untuk mendapatkan kondisi yang optimal untuk deteksi DENV dan analisis validasi tehnik in house multiplek realtime Reverse Transcriptase-Polimerase Chain Reaction (rRT-PCR) sehingga dapat digunakan untuk deteksi dini dan cepat infeksi DENV. Rancangan penelitian ini adalah penelitian eksperimental laboratorium. Strain standar DENV diisolasi dengan kit Roche® dan spesimen diekstraksi dengan kit Qiagen®. Strain standar DENV digunakan untuk optimasi suhu annealing dan konsentrasi primer masing-masing serotipe DENV dengan metode in house multiplek rRT-PCR berbasis SYBR green. Sebagai pembanding digunakan RT-PCR konvensional dengan menggunakan primer di daerah C-PrM. Primer in house multiplek rRT-PCR didesain di daerah envelope pada masing masing serotipe. Analisis limit of detection (LOD) dilakukan dengan pengenceran titer virus 105, 104, 103, 102, 10 dan 1 FFU/ml (in house multiplek rRT-PCR) pada keempat serotipe. Hasil in house multiplek rRT-PCR dibandingkan dengan hasil RT-PCR konvensional Lanciotti pada pasien dengue yang telah masuk dalam kriteria inklusi dan eksklusi. Suhu annealing optimal didapatkan pada suhu 58oC sedangkan konsentrasi optimal masing-masing primer untuk in house multiplek rRT-PCR adalah 4 pmol. LOD RNA pada DENV-1, DENV-2, DENV-3, DENV-4 adalah 1 FFU/ml, 10 FFU/ml, 104 FFU/ml dan 1 FFU/ml. In house multiplek rRT-PCR dibandingkan RT-PCR konvensional mempunyai sensitivitas sebesar 100%, spesifisitas 94,2 %, nilai prediksi positif 85,7% dan nilai prediksi negatif 100 %. In house multiplek rRT-PCR berbasis SYBR green merupakan metode yang dini, cepat dan tepat untuk deteksi DENV pada awal infeksi dengan sensitivitas dan spesifisitas yang baik sebagai metode diagnostik infeksi dengue dimasa mendatang.

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Dengue virus (DENV) is an infectious disease that is endemic in 100 countries in the World. Dengue virus infections can cause primary and secondary. Secondary infection is estimated to be more severe DHF and SDD. Developed diagnostic technique that no one has been able to quickly and accurately detect the infection early, especially for the Indonesian strain of dengue virus. The purpose of this study is to obtain optimal conditions for the detection of dengue infection and analysis techniques in-house validation of multiplex real-time Reverse Transcriptase-Polymerase Chain Reaction (rRT-PCR) for the Indonesian strain of dengue virus.

Design of this study is an experimental research laboratory. Standard strains of dengue virus was isolated with a kit Roche® and the specimen was extracted with Qiagen® kit. Standard strains of dengue virus is used for optimization primer annealing temperature and the concentration primers of each serotype dengue virus by multiplex rRT-PCR method based on SYBR green. Primers for RT-PCR conventional based lanciotii et al while rRT-PCR primer was designed in the envelope gen at each serotype. Limit of detection (LOD) by diluting the virus titer 105, 104, 103, 102 FFU /ml performed by rRTPCR in all four serotypes.

The results of multiplex rRT-PCR compared with results of conventional RT-PCR in patients with dengue lanciotti superbly into the criteria inclusion and exclusion. Optimal annealing temperature results obtained at a temperature of 58oC and optimal primer concentration of 4 pmol of each primer for 25 ul total reaction. LOD RNA in DENV-1, DENV-2, DENV-3 and DENV-4 at titer of 1 FFU / ml, 10 FFU/ml, 104 FFU/ml and 1 FFU/ml. .In house multiplex rRT-PCR compared with RT-PCR has a sensitivity of 100%, specificity 95,2%, positive predictive value 85,7% and negative predictive value of 100%. In-house multiplex rRT-PCR with SYBR green-based research is a method that is rapid and precise detection of dengue virus in early infection with good sensitivity and specificity compared to RT-PCR as a diagnostic method in the future dengue infection."

"ABSTRAK Latar belakang : Cytomegalovirus (CMV) merupakan salah satu infeksi oportunistikpada pasien dengan sindrom immunodefisiensi (AIDS). Gejala klinis dan CT scantidak dapat menegakkan diagnosa definitif ensefalitis CMV. Oleh karena itudiperlukan uji alternatif untuk menegakkan diagnosis infeksi CMV pada pasien HIVdengan infeksi otak. Salah satu uji yang sensitif dan spesifik adalah Real TimePolymerase Chain Reaction (rPCR).Tujuan : Mendapatkan uji deteksi molekular CMV pada pasien HIV dengantersangka infeksi otak.Metode : Penelitian dilakukan dalam 3 tahap. Tahap 1 adalah optimasi konsentrasiprimer, probe, suhu annealing, volume elusi ekstraksi DNA, dan volume cetakan.Tahap 2 adalah uji spesifisitas (reaksi silang) dan uji sensitivitas (ambang batasdeteksi DNA) rPCR dan tahap 3 adalah penerapan uji rPCR yang sudah dioptimasiterhadap sampel plasma, urin, dan LCS.Hasil : Kondisi optimal uji rPCR telah diperoleh dengan konsentrasi primer danprobe 0,1 μM, dengan kondisi suhu reaksi rPCR: aktivasi enzim pada 950C selama 3menit; 45 siklus pada 950C selama 15 detik (denaturasi) dan 560C selama 1 menit(annealing dan ekstensi). Volume elusi ekstraksi DNA yang optimal untuk ketigajenis sampel (LCS, plasma dan urin) adalah 40 μL, dan volume cetakan rPCR untukLCS, plasma, dan urin, masing-masing adalah 5, 4, dan 3 μL. Uji rPCR mampumendeteksi DNA pada 50.000 jumlah kopi/mL dan tidak bereaksi silang denganStaphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus,Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Mycobacteriumtuberculosis, Candida spp, Toxoplasma gondii, EBV,HSV,dan VZV. Penerapan ujirPCR pada sampel klinis memberikan hasil negatif pada semua sampel LCS, 72,22%positif pada sampel plasma, dan 72,22% positif pada sampel urin.Kesimpulan: Telah dilakukan optimasi uji rPCR dengan minimal deteksi DNACMV 50.000 jumlah kopi/mL dan tidak bereaksi silang dengan mikroorganisme yangberpotensi menyebabkan positif palsu (false positive).ABSTRACT Background: Cytomegalovirus (CMV) is one of opportunistic infections in patientswith Aquired Immunodeficiency Syndrome (AIDS). Clinical manifestations are nottypical, and CT scans can not define encephalitis CMV specifically. Therefore, it isimportant to apply an alternative assay for sensitive and specific detection of CMVinfection in HIV patients with suspected central nervous system (CNS) infections.One of the assays is real time polymerase chain reaction (rPCR).Objective: To obtain a molecular assay for detection of CMV in HIV patients withsuspect CNS infections.Methods: This study was conducted in three phases. The first is optimization ofconcentrations of primers, probe, annealing temperature, final elution of DNAextraction, and volume of PCR template. The second is determinations of sensitivity(minimal detection of DNA) and specificity (cross-reaction) of the optimized rPCR,and the third is application of the rPCR for clinical samples of plasma, urine, andliquor cerebrospinal (LCS).Results: The rPCR reaction showed optimal concentrations of primers and probe at0.1 μM, with thermal cycler: 950C for 3 min (enzyme activation), followed by 45cycles of 950C for 15 sec (denaturation) and 560C for 1 min (annealing andextension). Final elution of DNA extraction was 40 μL and volume of PCR templatesfor urine, plasma, and LCS was 3, 4, and 5 μL, respectively. The rPCR had minimaldetection of DNA at 50,000 copies/mL and was not cross-reacted withStaphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus,Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Mycobacteriumtuberculosis, Candida spp, Toxoplasma gondii, Epstein-Bar Virus (EBV), HerpesSimplex Virus (HSV) and Varicella Zoster Virus (VZV). Application of rPCR forclinical samples showed that the rPCR yielded 72.22% positive for plasma or urine,and negative for all LCS samples.Conclusion: The rPCR has been optimized in this study with minimal DNA detectionat 50,000 copies/mL and was not cross-reacted with other microorganisms that arepotential to cause false positive results.;Background: Cytomegalovirus (CMV) is one of opportunistic infections in patientswith Aquired Immunodeficiency Syndrome (AIDS). Clinical manifestations are nottypical, and CT scans can not define encephalitis CMV specifically. Therefore, it isimportant to apply an alternative assay for sensitive and specific detection of CMVinfection in HIV patients with suspected central nervous system (CNS) infections.One of the assays is real time polymerase chain reaction (rPCR).Objective: To obtain a molecular assay for detection of CMV in HIV patients withsuspect CNS infections.Methods: This study was conducted in three phases. The first is optimization ofconcentrations of primers, probe, annealing temperature, final elution of DNAextraction, and volume of PCR template. The second is determinations of sensitivity(minimal detection of DNA) and specificity (cross-reaction) of the optimized rPCR,and the third is application of the rPCR for clinical samples of plasma, urine, andliquor cerebrospinal (LCS).Results: The rPCR reaction showed optimal concentrations of primers and probe at0.1 μM, with thermal cycler: 950C for 3 min (enzyme activation), followed by 45cycles of 950C for 15 sec (denaturation) and 560C for 1 min (annealing andextension). Final elution of DNA extraction was 40 μL and volume of PCR templatesfor urine, plasma, and LCS was 3, 4, and 5 μL, respectively. The rPCR had minimaldetection of DNA at 50,000 copies/mL and was not cross-reacted withStaphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus,Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Mycobacteriumtuberculosis, Candida spp, Toxoplasma gondii, Epstein-Bar Virus (EBV), HerpesSimplex Virus (HSV) and Varicella Zoster Virus (VZV). Application of rPCR forclinical samples showed that the rPCR yielded 72.22% positive for plasma or urine,and negative for all LCS samples.Conclusion: The rPCR has been optimized in this study with minimal DNA detectionat 50,000 copies/mL and was not cross-reacted with other microorganisms that arepotential to cause false positive results.;Background: Cytomegalovirus (CMV) is one of opportunistic infections in patientswith Aquired Immunodeficiency Syndrome (AIDS). Clinical manifestations are nottypical, and CT scans can not define encephalitis CMV specifically. Therefore, it isimportant to apply an alternative assay for sensitive and specific detection of CMVinfection in HIV patients with suspected central nervous system (CNS) infections.One of the assays is real time polymerase chain reaction (rPCR).Objective: To obtain a molecular assay for detection of CMV in HIV patients withsuspect CNS infections.Methods: This study was conducted in three phases. The first is optimization ofconcentrations of primers, probe, annealing temperature, final elution of DNAextraction, and volume of PCR template. The second is determinations of sensitivity(minimal detection of DNA) and specificity (cross-reaction) of the optimized rPCR,and the third is application of the rPCR for clinical samples of plasma, urine, andliquor cerebrospinal (LCS).Results: The rPCR reaction showed optimal concentrations of primers and probe at0.1 μM, with thermal cycler: 950C for 3 min (enzyme activation), followed by 45cycles of 950C for 15 sec (denaturation) and 560C for 1 min (annealing andextension). Final elution of DNA extraction was 40 μL and volume of PCR templatesfor urine, plasma, and LCS was 3, 4, and 5 μL, respectively. The rPCR had minimaldetection of DNA at 50,000 copies/mL and was not cross-reacted withStaphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus,Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Mycobacteriumtuberculosis, Candida spp, Toxoplasma gondii, Epstein-Bar Virus (EBV), HerpesSimplex Virus (HSV) and Varicella Zoster Virus (VZV). Application of rPCR forclinical samples showed that the rPCR yielded 72.22% positive for plasma or urine,and negative for all LCS samples.Conclusion: The rPCR has been optimized in this study with minimal DNA detectionat 50,000 copies/mL and was not cross-reacted with other microorganisms that arepotential to cause false positive results.;Background: Cytomegalovirus (CMV) is one of opportunistic infections in patientswith Aquired Immunodeficiency Syndrome (AIDS). Clinical manifestations are nottypical, and CT scans can not define encephalitis CMV specifically. Therefore, it isimportant to apply an alternative assay for sensitive and specific detection of CMVinfection in HIV patients with suspected central nervous system (CNS) infections.One of the assays is real time polymerase chain reaction (rPCR).Objective: To obtain a molecular assay for detection of CMV in HIV patients withsuspect CNS infections.Methods: This study was conducted in three phases. The first is optimization ofconcentrations of primers, probe, annealing temperature, final elution of DNAextraction, and volume of PCR template. The second is determinations of sensitivity(minimal detection of DNA) and specificity (cross-reaction) of the optimized rPCR,and the third is application of the rPCR for clinical samples of plasma, urine, andliquor cerebrospinal (LCS).Results: The rPCR reaction showed optimal concentrations of primers and probe at0.1 μM, with thermal cycler: 950C for 3 min (enzyme activation), followed by 45cycles of 950C for 15 sec (denaturation) and 560C for 1 min (annealing andextension). Final elution of DNA extraction was 40 μL and volume of PCR templatesfor urine, plasma, and LCS was 3, 4, and 5 μL, respectively. The rPCR had minimaldetection of DNA at 50,000 copies/mL and was not cross-reacted withStaphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus,Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Mycobacteriumtuberculosis, Candida spp, Toxoplasma gondii, Epstein-Bar Virus (EBV), HerpesSimplex Virus (HSV) and Varicella Zoster Virus (VZV). Application of rPCR forclinical samples showed that the rPCR yielded 72.22% positive for plasma or urine,and negative for all LCS samples.Conclusion: The rPCR has been optimized in this study with minimal DNA detectionat 50,000 copies/mL and was not cross-reacted with other microorganisms that arepotential to cause false positive results."

"Infeksi yang berhubungan dengan perawatan kesehatan atau infeksi yang diperoleh dalam perawatan kesehatan adalah efek samping yang paling umum dalam penyediaan layanan kesehatan di seluruh dunia. Rumah sakit bertujuan untuk memberikan perlindungan bagi keselamatan pasien, masyarakat, lingkungan rumah sakit dan sumber daya manusia di rumah sakit dan meningkatkan kualitas dan mempertahankan standar layanan rumah sakit. Program Pencegahan dan Pengendalian Infeksi (PPI) adalah upaya untuk memastikan perlindungan setiap orang dari kemungkinan tertular infeksi dari sumber- sumber publik dan saat menerima layanan kesehatan di berbagai fasilitas kesehatan. Tujuan penelitian ini adalah untuk menganalisis implementasi program pencegahan dan pengendalian infeksi di RSUP Persahabatan Jakarta. Desain penelitian adalah kualitatif dengan menggunakan metode wawancara mendalam terstruktur, telaah dokumen serta observasi dengan memakai lembaran observasi.Hasil penelitian menunjukkan bahwa Sumber Daya Manusia masih kurang, sarana dan prasarana belum berkesinambungan dibeberapa unit layanan, kepatuhan kebersihan tangan di kalangan peserta didik masih rendah, laporan mengenai infeksi daerah operasi masih belum optimal dan pencatatan serta pelaporan kegiatan program PPI belum disampaikan ke Kementerian Kesehatan.Implementasi program PPI di rumah sakit membutuhkan dukungan SDM, sarana prasarana yang berkesinambungan terutama untuk sarana prasarana kebersihan tangan, edukasi yang intens terhadap peserta didik dan karyawan rumah sakit akan kepatuhan kebersihan tangan dan sistem pencatatan dan pelaporan kegiatan PPI sesuai aturan yang ada.

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Infections related to healthcare or infections acquired in healthcare are the most common side effects in the provision of health services worldwide. The hospital aims to provide protection for the safety of patients, the community, the hospital environment and human resources in hospitals and improve the quality and maintain standards of hospital services. Infection Prevention and Control (IPC) is an effort to ensure the protection of everyone from the possibility of contracting infection from public sources and when receiving health services in various health facilities.The purpose of this study was to analyze the implementation of infection prevention and control in RSUP Persahabatan Jakarta. The research design is qualitative using in-depth structured interview method, document review and observation using observation sheets. The results showed that human resources were still lacking, facilities and infrastructure had not been sustainable in some service units, compliance with hand hygiene among students was still low, reports of infection in operating areas were still not optimal and recording and reporting of PPI activities had not been submitted to the Ministry of Health. The implementation of PPI in hospitals requires the support of human resources, sustainable infrastructure, especially for hand hygiene infrastructure, intense education for students and hospital employees for compliance with hand hygiene and a system for recording and reporting PPI activities according to existing regulations."

"Infeksi parasit usus seperti cacing usus masih menjadi masalah di Indonesia. Infeksi cacing usus digolongkan sebagai neglected tropical diseases dan 800 juta penderita di antaranya adalah anak-anak. Anak panti asuhan tinggal di lingkungan yang padat dan rentan mengalami infeksi cacing usus sehingga diperlukan informasi mengenai sikap dan perilaku pencegahan infeksi cacing usus. Tujuan penelitian ini adalah untuk mengetahui hubungan antara karakteristik demografi dengan sikap dan perilaku pencegahan infeksi cacing usus anak panti asuhan. Penelitian ini menggunakan desain potong lintang. Pengambilan data dilakukan pada tanggal 10 Juni 2012 di Jakarta Timur dengan menggunakan kuesioner berisikan 13 pertanyaan tentang sikap dan perilaku pencegahan infeksi cacing usus. Data diolah dengan program SPSS for mac versi 20 dan dianalisis dengan uji Kruskal-Wallis, Mann-Whitney dan Spearman. Hasil penelitian ini menunjukkan rerata skor penilaian sikap dan perilaku pencegahan infeksi cacing masing-masing 20,2 dan 29,1. Nilai maksimal dari skor penilaian sikap dan perilaku masing-masing 25 dan 40. Terdapat hubungan antara jenis kelamin, usia dan tingkat pendidikan dengan perilaku (p<0,05), serta jenis kelamin dengan sikap (p<0,05). Disimpulkan sikap anak panti asuhan terhadap pencegahan infeksi cacing usus tergolong baik dan jika akan memberikan edukasi maka perlu memperhatikan usia, tingkat pendidikan dan jenis kelamin.

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Intestinal parasite infection, intestinal helminths for instance, remains a major problem in Indonesia. Intestinal helminths infection is regarded as a neglected tropical diseases and 800 million children are infected globally. Orphanage live in a densely populated place and are susceptible to intestinal helminths infection, thus it is necessary to know attitude and behavior of intestinal helminths infection.

The purpose of this study is to understand the relationship between Attitude and Behavior of Street Children about Soil Transmitted Helminths Infection Prevention and demographic characteristic. This study used analytic cross sectional design. Data were collected on June 10th 2012 in East Jakarta using a questionnaire consists of 13 questions about attitude and behavior of intestinal helminths infection prevention. The data collected were processed using SPSS for Mac version 20 program and Kruskal-Wallis, Mann-Whitney and Spearman statistic test.

The results showed that attitude and behavior scores are 20,2 and 29,1, respectively. Maximum score of attitude and behavior are 25 and 40, respectively. The results indicated that there is a relationship of gender, age, and education level with behavior (p<0,05), and also gender with attitude (p<0,05). It can be concluded that orphanage children attitude of intestinal helminths infection prevention is good meanwhile the behavior is poor and education methods should consider age, education level and gender."