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"Telah dilakukan penelitian yang bertujuan untuk mengetahui potensi madu PS (Pollen Substitute) sebagai penurun kadar glukosa darah puasa dan post-prandial mencit (Mus musculus L.) jantan galur DDY. Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok hewan uji, yaitu kelompok kontrol normal (KK1) yang diberikan pakan standar dan akuades, kelompok kontrol perlakuan (KK2) yang diberikan aloksan dan akuades, dan 2 kelompok perlakuan (KP1 dan KP2) yang diberikan aloksan serta madu PS 10% dan 20%selama 14 hari berturut-turut. Pengukuran kadar glukosa darah dilakukan pada awal (t0) dan akhir penelitian (t14)dengan menggunakan glukometer. Hasil uji anava satu arah (P<0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap kadar glukosa darah semua hewan uji. Hasil uji LSD (P<0,05) menunjukkan bahwa kadar glukosa darah KP2 tidak berbeda nyata dengan KK1. Penurunan kadar glukosa darah puasa dan post-prandialKP2 secara berturut-turut mencapai 42,5% dan 39,75%.Berdasarkan hasil tersebut, maka dapat disimpulkan bahwa pemberian madu PS 20% dapat menurunkan kadar glukosa darah mendekati normal (mengacu pada KK1).

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The study has been conducted to know the hypoglycemic effect of PS (Pollen Susbtitute) honey administration on fasting and post-prandial blood glucose level of male-DDY mice (Mus musculus L.). Twenty four male mice were divided to four groups consisting of normal control group (KK1) which was administered with neither alloxan or PS honey; treatment control group (KK2) which was administered with alloxan; and two treatment groups (KP1 and KP2) which was administered with alloxan and PS honey 10% and 20%, respectively, within 14 consecutive days. Fasting (16 hours) and post-prandial (2 hours after eating) blood glucose level were measured using glucometer. One-way anova test (P<0,05) showed that fasting and post-prandial blood glucose level decreased significantly. Least significant difference (LSD) (P<0,05) test showed that only the administration of 20% PS honey (KP2) cause the significant decrease in both fasting and post-prandial blood glucose level. According to normal control group (KK1), the blood glucose level of KP2 has been reduced to its normal range. It is concluded that administration of 20% PS honey significantly lowered blood glucose level (fasting and post-prandial)."

"ABSTRAKTelah dilakukan penelitian tentang pengaruh pemberian madu PS (pollen substitute) terhadap gambaran histologi pulau Langerhans pankreas pada mencit (Mus musculus L.) jantan galur DDY yang diinduksi aloksan. Sebanyak 24 ekor mencit dibagi dalam 4 kelompok 6 ulangan, yaitu: kelompok kontrol normal (KK1), kelompok kontrol diabetes (KK2), kelompok perlakuan pemberian madu PS 10% (KP1), dan kelompok perlakuan pemberian madu PS 20% (KP2). Pencekokan madu PS dilakukan setiap hari selama 14 hari berturut-turut. Pada hari ke-15, mencit dikorbankan, organ pankreas diisolasi dan dibuat sediaan histologi menggunakan metode parafin dengan pewarnaan Hematoksilin Eosin (HE). Sediaan diamati secara mikroskopik menggunakan mikroskop cahaya dan mikroproyektor. Data rerata diameter pulau Langerhans mencit dan jumlah sel β pankreas adalah sebagai berikut: KKI (115,03 ± 4,94) µm, (59,47 ± 2,08); KK2 (51,09 ± 8,39) µm, (15,24 ± 2,54); KP1 (106,70 ± 4,75) µm, (40,89 ± 2,33); KP2 (114,24 ± 10,85) µm, (46,78 ± 3,2). Hasil uji LSD (P < 0,05) menunjukkan terdapat perbedaan nyata antara kelompok perlakuan dengan kelompok kontrol KK2. Hal ini berarti bahwa pemberian madu PS (pollen substitute) memengaruhi rerata diameter pulau Langerhans dan jumlah sel β pankreas pada dosis 10% dan 20%.

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ABSTRACTThe study was undertaken to assess the effect of PS (pollen substitute) honey intake on recovery diameter islet of Langerhans pancreas of alloxan-induced male-DDY mice (Mus musculus L.). Tweenty-four male mice were randomly devided into four groups, consisting of normal control group (KK1), treatment control group (KK2), two treatment groups (KP1 and KP2) which was administered with alloxan and PS honey in concentration of 10% and 20%, respectively. Treatments were carried out orally within 14 consecutive days. The mice were sacrified at day 15 (T15). Histology slides was made with paraffin method and stained with Haematoxyline Eosin (HE) and observed with microscope and microprojector. Mean of diameter of islet of Langerhans and pancreatic cell β number : KKI (115,03 ± 4,94) µm, (59,47 ± 2,08); KK2 (51,09 ±8,39) µm, (15,24 ± 2,54); KP1 (106,70 ± 4,75) µm, (40,89 ± 2,33); KP2 (114,24 ±10,85) µm, (46,78 ± 3,2). Least significant difference (LSD) (P < 0,05%) test showed a significant effect of treatment. The result demonstrated the potential beneficiary effect of PS (pollen substitute) Honey for recovery diameter of islet of Langerhans and pancreatic cell β number in concentration of 10% and 20%."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui potensi hepatoprotektif madu PS (Pollen Substitute) terhadap kadar SGPT dan SGOT pada mencit (Mus musculus L.) jantan galur DDY yang diinduksi karbon tetraklorida (CCl4). Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok hewan uji, yaitu kelompok kontrol normal (KK1) yang diberi pakan standar dan akuades, kelompok kontrol perlakuan (KK2) yang diberi akuades dan larutan karbon tetraklorida, dan dua kelompok perlakuan (KP1 dan KP2) yang diberi madu PS 10% dan 20% b/v dan larutan karbon tetraklorida. Hasil uji ANOVA (P< 0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap kadar SGPT dan SGOT mencit pada semua kelompok perlakuan. Hasil uji LSD (P<0,05) menunjukkan adanya perbedaan nyata kadar SGPT dan SGOT mencit antar kelompok perlakuan (KK1, KK2, KP1 dan KP2). Persentase penurunan kadar SGPT dan SGOT terbaik dicapai oleh KP1 yaitu sebesar 82.93% dan 85.25% mendekati kadar normal (mengacu pada KK1), jika dibandingkan dengan dosis 20% pada KP2. Dengan demikian, dosis optimum dicapai oleh KP1 yaitu sebesar 10%.

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The objective of this study was to screen for the potential hepatoprotective role of PS (Pollen Substitute) honey administration on male DDY mice (Mus musculus L.)?s SGPT and SGOT level in blood serum. Twenty four mice were divided into four groups consisting of normal control group (KK1) which administrated with aqudes and coconut oil; treatment control group (KK2) which administrated with aquades and carbon tetrachloride; and two treatment groups (KP1 and KP2) which administrated with PS honey 10% and 20% also carbon tetrachloride, respectively, for 14 consecutive days. SGPT and SGOT level was measured in day 15 using SGPT and SGOT kit assay in laboratory. ANOVA one way test (P<0.05) shows that SGPT and SGOT level were significantly different between groups of animals. LSD test (P<0.05) shows that there is significantly different of SGPT and SGOT levels between KK1, KK2, KP1 and KP2. According to normal control group (KK1), the decrease percentage of SGPT and SGOT level of KP1 is better than KP2, that is 82.93% dan 85.25%. Its concluded that administration of PS honey reached its optimum dosage at 10%."

"ABSTRAK

Telah dilakukan penelitian yang bertujuan mengetahui potensi hepatoprotektif madu PS terhadap kadar alkali fosfatase (ALP) mencit (Mus musculus L.) jantan galur DDY. Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok hewan uji, yaitu kelompok kontrol normal (KK1) yang diberikan akuades dan minyak kelapa; kelompok kontrol perlakuan (KK2) yang diberikan akuades dan CCl4; serta 2 kelompok perlakuan (KP1 dan KP2) yang diberikan madu PS 10% dan 20% selama 14 hari berturut-turut, kemudian CCl4 2 jam setelah pemberian madu terakhir. Darah diambil 24 jam setelah injeksi CCl4. Kadar ALP diukur dengan metode kolorimetri. Hasil uji anova satu arah (P<0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap kadar ALP semua hewan uji. Dibandingkan kadar ALP KK2, kadar ALP KP1 lebih rendah 30,5% dan KP2 lebih rendah 52,9%. Namun, uji LSD (P<0,05) menunjukkan hanya kadar ALP KP2 yang tidak berbeda nyata dengan KK1. Berdasarkan hasil tersebut, disimpulkan bahwa potensi hepatoprotektif madu PS 20% lebih besar dibandingkan madu PS 10%.

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ABSTRACT

The study has been conducted to know the hepatoprotective potency of PS honey administration on male-DDY mice’s alkaline phosphatase level of blood plasma. Twenty four male mice were divided into four groups, namely normal control group (KK1) which was administered with aquadest and coconut oil; treatment control group (KK2) which was administered with aquadest and CCl4; and two treatment groups which was administered with PS honey 10% (KP1) and 20% (KP2) within 14 consecutive days and three groups (KK2, KP1,and KP2) were injected with CCl4 on the 14th day. Alkaline phosphatase was measured based on colorimetry method. One-way anova test (P<0,05) showed that alkaline phosphatase levels were significantly different. Compared with KK2, the alkaline phosphatase levels of KP1 and KP2 were 30,5% and 52,9% lesser than KK2, consecutively. However, LSD test (P<0,05) showed that only alkaline phosphatase level of KP2 was not significantly different. In conclusion, dose 20% of PS honey is more potential on hepatoprotective than those of 10%.

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"Penelitian telah dilakukan untuk mengetahui potensi hepatoprotektif madu PS (Pollen Substitute) terhadap gambaran histologis hati mencit (Mus musculus) jantan galur DDY. Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok, yaitu kelompok kontrol normal (KK1) yang diberikan akuades dan minyak kelapa; kelompok kontrol perlakuan (KK2) yang diberikan akuades dan CCl4; serta 2 kelompok perlakuan (KP1 dan KP2) yang diberikan madu PS dosis 0,04 ml/20 g bb dan 0,08 ml/20 g bb selama 14 hari berturut-turut, kemudian diinjeksikan CCl4 2 jam setelah pemberian madu PS terakhir. Organ hati diisolasi 24 jam setelah injeksi CCl4. Hasil uji statistik menunjukkan tidak ada pengaruh pemberian madu PS terhadap berat basah organ hati (KK1 [1,57±0,34] gram, KK2 [1,92±0,21] gram, KP1[1,73±0,34] gram, KP2 [1,75±0,30] gram) dan diameter vena sentralis (KK1 [37,91±2,44] μm, KK2 [73,39±3,06] μm, KP1 [70,03±9,65] μm, KP2 [67,61±6,33] μm), serta terdapat pengaruh pemberian madu PS terhadap warna organ hati, persentase derajat kerusakan lobulus hati (derajat 0, 1, 2, dan 3 KK1 [55,70%; 40,00%; 4,33%; dan 0,00%], KK2 [4,56%; 29,00%; 18,67%; dan 47,78%], KP1 [14,22%; 43,11%; 15,22%; dan 27,44%], KP2 [6,34%; 41,33%; 24,67%; dan 27,67%]), dan gambaran histologis hati. Hasil pengamatan menunjukkan pemberian madu PS dosis 0,04 ml/20 g bb memberikan efek hepatoprotektif yang lebih baik dibandingkan madu PS dosis 0,08 ml/20 g bb terhadap gambaran histologis hati.

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The aim of this study was to know hepatoprotective potency of PS (Pollen Substitute) honey to liver histology of DDY strain male mice (Mus musculus). Twenty four male mice were divided into 4 groups, which were normal control group (KK1) which was given aquadest and palm oil, treatment control group (KK2) which was given aquadest and CCl4, as well as 2 treatment groups (KP1 and KP2) which were given PS honey dose 0,04 ml/20 g bb and 0,08 ml/20 g bb in 14 days. Then KP1 and KP2 were induced by CCl4 2 hours after the last administration of honey PS. The liver then was isolated 24 hours after CCl4 injection. The result of statistic test showed that there were no effects of PS honey administration to liver wet weight (KK1 [1,57±0,34] gram, KK2 [1,92±0,21]gram, KP1[1,73±0,34] gram; KP2 [1,75±0,30] gram) and central vein diameter (KK1 [37,91±2,44] μm, KK2 [73,39±3,06] μm, KP1 [70,03±9,65] μm, KP2 [67,61±6,33] μm), and there were effects of PS honey admisnistration to liver color, percentage of liver lobulus damage level (level 0, 1, 2, and 3 of KK1 [55,70%; 40,00%; 4,33%; and 0,00%], KK2 [4,56%; 29,00%; 18,67%; and 47,78%], KP1 [14,22%; 43,11%; 15,22%; and 27,44%], KP2 [6,34%; 41,33%; 24,67%; and 27,67%]), and liver histology. The result of the observation showed that the hepatoprotective effect of administration of PS honey dose 0,04 ml/20 g bw was better than dose 0,08 ml/20 g to liver histology.;"

"Telah dilakukan penelitian untuk mengetahui pengaruh pemberian madu PS (Pollen Substitute) terhadap konsentrasi trigliserida plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Dua puluh empat tikus jantan dibagi dalam empat kelompok, yaitu kelompok kontrol normal (KK1) yang tidak diberi diet tinggi lemak, kelompok kontrol perlakuan (KK2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan akuades, dan dua kelompok perlakuan (KP1 dan KP2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan madu PS dengan dosis masing-masing 10% dan 20% b/v. Bahan uji diberikan setiap hari selama 14 hari berturut-turut. Hasil uji anava satu faktor (P < 0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap konsentrasi trigliserida akhir pada semua kelompok perlakuan. Hasil uji LSD (P<0,05) menunjukkan penurunan konsentrasi trigliserida pada seluruh kelompok perlakuan (KP1 dan KP2). Konsentrasi trigliserida pada kelompok perlakuan yang mendekati kelompok kontrol normal dicapai oleh kelompok perlakuan dengan dosis 20% b/v.

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The research was done to observe the effect of honey PS (Pollen Substitute) on the concentration of triglyceride in blood plasma of male rat (Rattus norvegicus L.) Sprague-Dawley strain. Twenty-five male rats were divided into four groups, consisting of normal control group (KK1) that was not given a high fat diet, treatment control group (KK2) that was given a high fat diet, 0.01% propylthiouracil (PTU) solution, and two treatment groups (KP1 and KP2) that were given a high fat diet, 0.01% propylthiouracil (PTU) solution, and honey PS with dose of 10% and 20% w/v, respectively, for 14 consecutive days.

The result of anova test (P < 0.05) showed significant effect of honey PS toward triglyceride concentration in all treatment groups. LSD test (P < 0,05) showed that the decreased blood total cholesterol concentration occurred at both treatment groups (KP1 and KP2). The concentration of triglyceride in the treatment group that approached the normal control group achieved by the treatment group with dose of 20% w/v."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui potensi
antihiperglikemik simplisia Alphitobius sp. pada mencit (Mus musculus L.) yang diinduksi aloksan monohidrat (250 mg/kg bb). Dua puluh lima ekor mencit jantan, dibagi ke dalam 5 kelompok, terdiri atas kelompok kontrol normal yang diberi akuades (KK1), kelompok kontrol perlakuan yang diinduksi aloksan dan diberi akuades (KK2), kelompok perlakuan yang diinduksi aloksan dan diberi simplisia
dosis 0,5; 1,05; dan 1,6 mg/kg bb (KP1; KP2; KP3). Pemberian simplisia
dilakukan selama 14 hari beruturut-turut. Hasil uji ANAVA (P < 0,05)
menunjukkan adanya pengaruh pemberian simplisia terhadap kadar glukosa darah puasa dan post prandial mencit. Hasil uji LSD (P < 0,05) menunjukkan tidak ada perbedaan nyata antara KK1 dengan KP2 dan KP3, tetapi ada perbedaan nyata antara KK2 dengan KK1,KP2 dan KP3. Pemberian simplisia dosis 1,05 dan 1,6 mg/kg bb mampu menurunkan kadar glukosa darah puasa sebanyak 29,91% dan 37,04%, dan post prandial sebanyak 49,51%, dan 52,32% ke keadaan normal (KK1 sebagai acuan).

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The study has been conducted to know antihyperglycemic effect of simplicia Alphitobius sp. on alloxan (250 mg/kg bb) induced male DDY mice (Mus musculus L.). Twenty five mice were divided into 5 groups consisting, normal control group which was administered with aquades (KK1), treatment control group which was induced by alloxan and given aquades (KK2), treatment groups which were induced by alloxan and given 0,5; 1,05; 1,6 mg/kg bb (KP1; KP2; KP3) dose of simplicia. Simplicia were given consecutively for 14 days. One-way anova test result (P < 0,05) showed that there was difference in fasting and post
prandial glucose level among those groups. Least Signficant Difference (LSD) test (P < 0,05) showed that there was no difference between KK1 - KP2 and KP3. But, there was difference between KK2 - KK1, KP2, and KP3. Result showed that simplicia’s dose 1,05 and 1,6 mg/kg bb were able to lower fasting blood glucose level up to 29,91% and 37,04%, also post prandial glucose level up to 49,51% and 52,32% to normal control (KK1) state."

"Uji aktivitas antivirus ekstrak etanol daun angsana (Pterocarpus indicus Willd.) menyatakan bahwa daun angsana berpotensi sebagai media pengobatan demam berdarah. Penelitian bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol daun angsana dengan tingkatan dosis 0,5 , 5, 50, dan 500 mg/kg bb terhadap morfologi fetus mencit galur DDY. Bahan uji diberikan secara oral sejak hari ke-6 hingga hari ke-15 kebuntingan. Induk mencit dikorbankan dan dibedah caesar pada hari ke-18 kebuntingan. Pengamatan meliputi jumlah corpus luteum, jumlah kematian dan resorpsi, jenis kelamin fetus, penimbangan berat badan fetus, pengukuran panjang fetus, serta pengamatan visual terhadap kelengkapan morfologi fetus. Hasil uji anava (P>0,05) menyatakan bahwa pemberian ekstrak etanol daun angsana tidak memengaruhi jumlah implantasi, berat dan panjang badan fetus, serta jenis kelamin fetus. Hasil uji Kruskal-Wallis (P>0,05) menunjukkan bahwa tidak ada pengaruh nyata terhadap jumlah fetus hidup, mati, resorpsi dan kelainan morfologi, sedangkan hasil uji Mann-Whitney (P>0,05) terhadap berat plasenta menunjukkan ada pengaruh nyata akibat pemberian ekstrak etanol daun angsana.

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Antiviral activity of ethanol extract of angsana leaves (Pterocarpus indicus Willd.) showed that angsana potentially as dengue fever treatment. This study was performed to examine the influence of ethanol extract of angsana leaves at dose level 0,5 , 5, 50, and 500 mg/kg bw to mice fetus DDY strain. Substance test given by oral at 6th until 15th gestation period. At the 18th gestation, mice were euthanased by caesarian sectioned. Observation include the number of corpus luteum, number of death and resorption, weight body and crown-rump of fetus, and observation to completeness of morphological visually.

Anava test (P>0,05) showed that ethanol extract of angsana leaves did not influence the implantation number, fetus weight and crown-rump, and fetus sex. Kruskal-Wallis test (P>0,05) showed that there is no significant difference on alive, dead, and resorption fetus; and morphological abnormality, whereas Mann-Whitney test (P>0,05) on placenta weight showed there is significant difference due to administration of ethanol extract of angsana leaves."

"Penelitian tentang potensi aktivitas antikanker ekstrak kasar Holothuria atra di Indonesia belum pernah dilakukan. Oleh karena itu, telah dilakukan penelitian untuk menguji potensi antikanker ekstrak kasar H. atra menggunakan uji mikronukleus dari sumsum tulang Mus musculus jantan galur DDY yang sebelumnya telah diinduksi 0,66 mg/kg bb kolkisin secara intraperitoneal. Hasil pengujian aktivitas antikanker secara in vivo menunjukkan bahwa pemberian ekstrak kasar H. atra dengan dosis 0,33; 0,66; 0,99; dan 1,32 g/kg bb selama 7 hari secara oral mampu menurunkan frekuensi sel eritrosit polikromatik (PCE) bermikronukleus secara nyata (p < 0,05) yang diamati melalui preparat apusan sumsum tulang paha mencit. Dosis 0,33; 0,66; 0,99; dan 1,32 g/kg bb secara berurutan mampu menurunkan frekuensi sel PCE bermikronukleus sebanyak 51,24%; 59,70%; 63,68%; dan 68,66%. Namun, dosis ekstrak kasar H. arta yang optimum belum ditemukan. Berdasarkan hasil pengujian tersebut diperoleh kesimpulan bahwa ekstrak kasar H. atra mempunyai potensi aktivitas antikanker.

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Research on potential anticancer activity of Holothuria atra crude extracts in Indonesia has never been done. Therefore, we conducted a study to test the anticancer potential of H. atra crude extract using the micronucleus test of male Mus musculus strain DDY bone marrow who had previously been induced by 0.66 mg/kg bw colchicines intraperitoneally. Our data showed that treatment of H. atra crude extract at dose 0,33; 0,66; 0,99, and 1,32 g/kg bw for 7 days orally can reduce the frequency of micronucleus in polychromatic erythrocytes cells (PCE) from bone marrow smears. Dose of 0,33; 0,66; 0,99, and 1,32 g/kg bw in sequence can reduce the frequency of micronucleus in PCE as much as 51,24%; 59,70%; 63,68%; dan 68,66%. However, the optimum doses of H. atra crude extract has not been found. Based on these results we conclude that H. atra crude extract has potential anticancer activity."

"Telah dilakukan penelitian untuk mengetahui aktivitas ekstrak biji kacang merah (Vigna angularis) sebagai inhibitor enzim α-amilase secara in vitro dan in vivo. Biji kacang merah (Vigna angularis) diekstrak dengan PBS (Phosphate Buffer Saline) kemudian difraksinasi dengan amonium sulfat. Metode in vitro dilakukan dengan mengamati persentase inhibisi pada masing-masing fraksi ekstrak. Tahap selanjutnya dilakukan uji in vivo dengan metode tes toleransi glukosa oral (TTGO). Hasil persentase inhibisi tertinggi pada uji in vitro yaitu terdapat pada fraksi endapan amonium sulfat 0--60% yaitu sebesar 72,39%. Persentase inhibisi pada ekstrak kasar sebesar 52,64%. Uji in vivo dilakukan pada ekstrak kasar dengan pertimbangan tidak ada perbedaan persentase inhibisi secara bermakna dibandingkan dengan fraksi endapan ammonium sulfat 0?60%. Tikus dikelompokkan menjadi 5 kelompok, yaitu kelompok kontrol normal, kontrol positif, kontrol negatif, kelompok dosis 1 (600 mg/300 g bb), dan dosis 2 (800 mg/300 g bb). Pengukuran kadar glukosa darah postprandial dilakukan pada menit ke-30, 60, dan 120 setelah pemberian ekstrak. Analisis protein ekstrak kacang merah (Vigna angularis) dengan metode SDS-PAGE elektroforesis menunjukkan ukuran protein dari phaseolamin sebesar ±55,9 kDa pada setiap fraksi ekstrak. Hasil penelitian uji in vivo menunjukkan bahwa ekstrak kasar biji kacang merah (Vigna angularis) tidak menunjukkan efek penurunan kadar glukosa darah.

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The research was done in order to determine the activity of a mixture extract adzuki bean (Vigna angularis) for α-amylase inhibitor by in vitro and in vivo method. Adzuki bean (Vigna angularis) seeds was extracted with PBS (Phosphate buffer saline) and fractionated with ammonium sulphate, then percentage of inhibition of each fraction was observed. In vivo study was done with oral glucose tolerance test method. The in vitro result showed that highest activity in fractination with ammonium sulphate was founded in saturation level of 0--60%, α-amylase were inhibited 72,39 %. Percentage of inhibition of crude extract is 52,64 %. In vivo method was done with crude extract because there is no significant difference in the percentage of inhibition with ammonium sulphate fraction. Male rats were divided into five group. They were normal control group, negative control group, positive control group, and the other 2 group given the extract (600 mg/300 g body weight and 800 mg/300 g body weight). Glucose level was measured in 30, 60 and 120 minutes post glucose administration. Electrophoresis analysis of extract by SDS PAGE showed that the size of phaseolamin was ±55,9 kDa. The result of this study showed that crude extract of adzuki bean (Vigna angularis) has no effect on lowering blood glucose level."