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"Penyakit Panama pada tanaman pisang disebabkan oleh kapang patogen Fusarium oxysporum f. sp. cubense. Telah dilakukan penelitian untuk karakterisasi penghambatan Aktinomisetes terhadap Fusarium oxysporum f. sp. cubense secara in vitro menggunakan sel hidup dan filtrat kultur bebas sel. Isolat Aktinomisetes LAI-I dan L31 diketahui menghasilkan enzim kitinase, protease, dan antibiotik yang dapat menghambat pertumbuhan, menyebabkan perubahan morfologi hifa berupa penebalan pada ujung-ujung hifa, serta menghambat germinasi spora Fusarium oxysporum f. sp. cubense. Perhitungan statistik menunjukkan adanya perbedaan yang nyata antara kelompok kontrol, perlakuan LAI-I, dan perlakuan L31. Hasil tersebut memperlihatkan adanya kemampuan isolat LAI-I dan L31 menghambat Fusarium oxysporum f. sp. cubense.

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The Panama disease in banana plants is caused by the pathogenic fungi Fusarium oxysporum f. sp. cubense. Research has been conducted to characterize the inhibition mechanism of Actinomycetes towards Fusarium oxysporum f. sp. cubense in vitro by using living cell and cell-free culture filtrate. Actinomycetes isolates LAI-I and L31 produce chitinase enzyme, protease enzyme, and secondary metabolites that can inhibit the growth, lead morphological changes of hyphae as swollen at the end of the hypha, and inhibit spore germination of Fusarium oxysporum f. sp. cubense. The statistic reveals the significant differences between control, LAI-I treatment, and L31 treatment. The result shows the ability of isolate LAI-I and L31 to inhibit Fusarium oxysporum f. sp. cubense."

"Pisang Cavendish (Musa acuminata Colla) merupakan salah satu kultivar pisang yang menghadapi ancaman serius berupa serangan penyakit layu Fusarium yang disebabkan oleh infeksi kapang patogen Fusarium oxysporum f. sp. cubense (Foc). Penyakit layu Fusarium menyebabkan penurunan penurunan kaualitas dan produksi tanaman pisang. Penggunaan mikroorganisme sebagai biokontrol dapat dikembangkan untuk alternatif fungisida dalam pengendalian penyakit layu Fusarium di tanaman pisang. Streptomyces spp. diketahui mampu memproduksi senyawa metabolit yang bersifat antifungi dan menekan pertumbuhan patogen. Tujuan dari penelitian ini adalah untuk memperoleh, mengetahui aktivitas biokontrol, dan mengidentifikasi isolat yang diduga sebagai Streptomyces spp. yang diisolasi dari PT Green Giant Pineapple (GGP), Lampung Timur. Metode yang dilakukan dalam penelitian ini adalah pengujian secara in vitro yang meliputi uji aktivitas enzim protease, kitinase, selulase, peroksidase, siderofor, amonia, HCN, dan katalase. Pengujian dilanjutkan dengan identifikasi secara molekuler dan uji kompatibilitas antarisolat. Isolat 1H31, 2H44, 3H32, 3H42, dan 5H5 berhasil diisolasi dari tanah Perkebunan pisang. Isolat terseleksi mampu menghambat kapang Foc dan saling kompatibel. Isolat 3H42 dan 5H5 menunjukkan aktivitas biokontrol yang paling baik yang diketahui dari hasil positif yang ditunjukkan pada semua uji aktivitas biokontrol yang dilakukan. Kelima isolat terseleksi diketahui memiliki kemiripan secara morfologis dan filogenetik dengan S. malaysiensis, S. scabei, S. cameroonensis, S. abikoensis, dan S. katrae.

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Cavendish banana (Musa acuminata Colla) is one of the banana cultivars that faces a serious threat in the form of Fusarium wilt disease caused by infection with the pathogenic fungus Fusarium oxysporum f. sp. cubense (Foc). Fusarium wilt disease causes a decrease in the quality and production of banana plants. The use of microorganisms as biocontrol can be developed as an alternative to fungicides to control Fusarium wilt disease in banana plants. Streptomyces spp. is known to produce metabolite compounds that are antifungal and suppress pathogen growth. The purpose of this study was to obtain, determine the biocontrol activity, and identify isolates suspected as Streptomyces spp. isolated from PT Green Giant Pineapple (GGP), East Lampung. The method used in this research is in vitro testing which includes enzyme activity tests of protease, chitinase, cellulase, peroxidase, siderophore, ammonia, HCN, and catalase. The test was continued with molecular identification and compatibility test between isolates. Isolates 1H31, 2H44, 3H32, 3H42, and 5H5 were successfully isolated from banana plantation soil. The selected isolates were able to inhibit Foc mold and were mutually compatible. Isolates 3H42 and 5H5 showed the best biocontrol activity known from the positive results shown in all biocontrol activity tests conducted. The five selected isolates are known to have morphological and phylogenetic similarities with S. malaysiensis, S. scabei, S. cameroonensis, S. abikoensis, and S. katrae."

"Kendala yang sering dihadapi pada pertanian adalah hama tanaman yang dapat mengganggu produktivitas tanaman pangan. Pemberantasan hama sering menggunakan pestisida kimiawi yang dapat berdampak buruk pada lingkungan dan tanaman. Salah satu alternatif mengatasi permasalahan tersebut adalah dengan menggunakan mikroorganisme sebagai biokontrol bagi hama tanaman. Mikroorganisme diketahui berpotensi sebagai agen biokontrol, dan aktivitas tersebut dapat diuji dengan  Antibiosis. Aktivitas antibiosis terlihat sebagai kemampuan menghambat pertumbuhan suatu mikroorganisme terhadap mikroorganisme lain. Pada penelitian ini dilakukan uji antibiosis dan aktivitas enzim dari empat isolat basil Gram negatif yaitu TTM, TTO, TKL, TTH, terhadap fungi Fusarium oxysporum dan Ganoderma boninense. Keempat isolat bakteri difermentasikan dalam medium Nutrient broth (NB) selama 6, 9, dan 12 hari, pada suhu inkubasi 39oC. Uji antibiosis dilakukan secara kualitatif dengan menggunakan cylinder diffusion method. Hasil uji antibiosis menunjukkan bahwa hanya isolat TTH dan TTM yang memiliki potensi untuk menghambat fungi Ganoderma boninense  dalam fermentasi hari ke 6,9, dan 12. Hasil uji aktivitas enzim menunjukkan bahwa hanya isolat TTO yang memiliki aktivitas kitinolitik.

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One of the common challenges in agriculture is the presence of plant pests that can disrupt the productivity of food crops. Pest control often relies on the use of chemical pesticides, which can have negative impacts on the environment and plants. One alternative to address this issue is the utilization of microorganisms as biocontrol agents for plant pests. Microorganisms are known to have the potential as biocontrol agents, and this activity can be tested through antibiosis. Antibiosis activity is observed as the ability to inhibit the growth of one microorganism by another. In this study, antibiosis and enzyme activity tests were conducted on four isolates of Gram-negative bacteria, namely TTM, TTO, TKL, and TTH, against the fungi Fusarium oxysporum and Ganoderma boninense. The four bacterial isolates were fermented in Nutrient Broth (NB) medium for 6, 9, and 12 days at an incubation temperature of 39°C. The antibiosis test was qualitatively performed using the cylinder diffusion method. The results of the antibiosis test showed that only the TTH and TTM isolates had the potential to inhibit the Ganoderma boninense fungi during the 6th, 9th, and 12th days of fermentation. The enzyme activity test results indicated that only the TTO isolate exhibited chitinolytic activity."

"pisang raja bulu is one of the most important bananas in Indonesia. However,, this plant low tolerance to wilt disease, caused by fusarium oxysporum f. cubense. Its mass cultivation is inhibited by the absence of variety tolerant to the disease. A wide range of genetic variability will be needed if selection for novel characters is to be conducted, especially when there is no source of resistance gene available for breeding materials. This research consisted of callus induction from primary explant, induction of somaclonal variation using gamma iradiation, and in vitro selection using fusaric acid, followed by regeneration and acclimatization of selected plantlets. The media applied for callus induction was MS (Murashige and skoog. 1962) +2,4-D 1 and 3 mg/l + NAA 0 and 0,1 mg/l and 2,4-D 5 mg/l + BA 0,5 mg/l + casein hidrolysate (CH) 500 mg/l. The applied gamma irradiation dosage were 0, and 0,1 mg/l and 2,4-D 5 mg/l + BA 0,5 mg/l + casein hidrolysate (CH) 500 mg/l. The applied gamma irradiation dosage were 0, 5.0, 7.5, 10 and 15 Gy. The irradiated cali was subsequently subcultures on selection media i.e., MS containing fusaric acid at 30 and 45 mg/l. The living calli was then regenerated on media containing BA, TDZ, eith or without proline and arginine. In addition, MS + kinetin 5 mg/l + 1AA 0,2 mg/l was applied for shoot development. The result showed that the most suitable callus induction media for pisang raja bulu was MS +2,4-D 5 mg/l +BA 0,5 mg/l +CH 500 mg/l. The gamma irradiation of 10 Gy produced somaclone lines which were able to proliferate bud nodules on selection media containing fusaric acid at 30 and 45 mg/l. The media for shoot development was MS + kinetin 5 mg/l + 1AA 0,2 mg/l. Plantlet obtained form the in vitro were then successfully acclimatized in the green house."

"Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW diisolasi dari tanah dan rizosfer tanaman dari perkebunan nanas dan pisang di Provinsi Lampung. Kedua bakteri tersebut diuji kemampuannya dalam melawan patogen Fusarium oxysporum Schlecht f. sp. cubense (Foc) secara in-vitro dan in-vivo. Aplikasi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW sebagai isolat tunggal maupun kombinasinya secara in-vivo pada tanaman pisang var. Cavendish dilakukan dalam rumah kaca selama 30 hari. Tujuan dari penelitian ini adalah untuk mengkarakterisasi dan menguji potensi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW sebagai agen biokontrol dalam menghambat patogen Foc dan mengkaji kemampuannya untuk menghasilkan enzim ketahanan tanaman pisang. Potensi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW sebagai agen biokontrol ditunjukkan dengan kemampuannya dalam menghambat pertumbuhan patogen Foc, sintesis enzim protease dan kitinase ekstraseluler, produksi hormon tumbuh Indole-Acetic Acid (IAA), dan produksi enzim ketahanan tanaman phenylalanine ammonia-lyase (PAL) and tyrosine ammonia lyase (TAL). Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW juga berperan sebagai plant growth-promoting rhizobacteia (PGPR), yang diindikasikan dengan peningkatan pertumbuhan tanaman pisang, di mana perlakuan Streptomyces L.3.1-DW memiliki rata-rata tinggi tanaman tertinggi dibandingkan perlakuan lainnya, dengan atau tanpa infeksi Foc. Hasil penelitian secara in-vitro dan in-vivo menunjukkan bahwa Streptomyces sp. L.3.1-DW memiliki kemampuan sebagai agen biokontrol yang lebih baik dibandingkan Bacillus sp. 140-B. Penelitian ini mengindikasikan bahwa Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW dapat digunakan sebagai alternatif untuk mengendalikan infeksi Foc.

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Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were isolated from soil and rhizosphere area of pineapple and banana plantation in Lampung Province. Those bacteria were evaluated in-vitro and in-vivo tests againts Fusarium oxysporum Schlecht f. sp. cubense (Foc). Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or in combination in banana plant var. Cavendish were tested under greenhouse conditions for 30 days.

The aims of this study were to characterize and investigate the potentials of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents to inhibit Foc pathogen and investigate their abilities to produce plant resistancy enzymes. The potentials of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents were showed by their abilities to inhibit growth of Foc pathogen, synthesize extracellular protease and chitinase enzymes, produce growth hormone, such as Indole-Acetic Acid (IAA), and produce plant resistancy enzymes, such as phenylalanine ammonia-lyase (PAL) and tyrosine ammonia lyase (TAL). Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW also act as plant growth-promoting rhizobacteia (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.

In-vitro and in-vivo tests was showed that Streptomyces sp. L.3.1-DW had better biocontrol activities compared to Bacillus sp. 140-B. This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW could be used as alternative solutions to control Foc pathogen."

"Tujuan penelitian adalah mengetahui aktivitas amilolitik 17 isolat 'Actinobacteria' termofilik pada suhu tinggi, dan memperoleh informasi spesies, dan posisi filogenetik isolat potensial berdasarkan data sekuens gen 16S rRNA, analisis filogenetik, karakterisasi morfologi, fisiologi, dan biokimia. Kemampuan tumbuh 17 isolat 'Actinobacteria' termofilik pada berbagai variasi suhu diuji menggunakan medium ISP 1 agar, dan diinkubasi pada suhu 45, 50, 55, 60 ^oC selama 7 hari. Berdasarkan hasil penelitian, 17 isolat memiliki pertumbuhan yang bervariasi pada suhu 45--60 ^oC. Tujuh belas isolat tumbuh pada suhu inkubasi 45 ^oC, 16 isolat pada suhu 50 ^oC, enam isolat pada suhu 55 ^oC, dan lima isolat pada suhu 60 ^oC terdiri atas SL1-2-R-2, SL1-2-R-3, SL1-2-R-4, SL2-2-R-15, dan SL3-1-R-16. Aktivitas amilolitik 17 isolat 'Actinobacteria' termofilik pada berbagai variasi suhu diuji dengan metode 'starch agar plate', menggunakan medium Minimal (Mm) agar dengan penambahan pati ('soluble starch') sebagai substrat sebanyak 1% (b/v), dan diinkubasi pada suhu 45, 50, 55, dan 60 ^oC selama 7 hari. Aktivitas amilolitik yang positif ditandai dengan terbentuknya zona bening di sekitar koloni bakteri setelah diteteskan larutan 'Lugol's iodine' sebanyak 1,5 ml. Hasil penelitian menunjukkan bahwa sebagian besar isolat yang diperoleh dari tanah di dekat geiser Cisolok memiliki aktivitas amilolitik yang bervariasi pada suhu 45--60 ^oC. Lima belas isolat memiliki aktivitas amilolitik pada suhu 45 ^oC, 13 isolat pada suhu 50 ^oC, empat isolat pada suhu 55 ^oC, dan hanya tiga isolat pada suhu 60 ^oC. Namun demikian, dua isolat (SL2-2-R-15 dan SL3-1-R-16) tidak memiliki aktivitas amilolitik pada suhu 45, 50, dan 55 ^oC setelah diinkubasi selama 7 hari. Tiga isolat potensial yang memiliki aktivitas amilolitik pada suhu 60 ^oC (SL1-2-R-2, SL1-2-R-3, dan SL1-2-R-4), berdasarkan data sekuens gen 16S rRNA, analisis filogenetik, dan karakterisasi fenotipik tiga isolat potensial tersebut diidentifikasi sebagai 'Actinomadura keratinilytica'.

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The aims of this study were to screen for amylolytic activity of the 17 themorphilic 'Actinobacteria' at high temperature, and to obtain species information and phylogenetic position based on 16S rRNA gene sequence, phylogenetic analysis, morphological, physiological, and biochemical characterizations. The ability to grow at various temperature was carried out on ISP 1 agar medium, incubated at 45, 50, 55, 60 ^oC for 7 days. The results showed that the 17 isolates 'Actinobacteria' have varying growth at a temperature of 45--60 ^oC. Seventeen, 16, and six isolates grew at 45, 50, and 55 ^oC, respectively, and only five isolates grew at 60 ^oC, designated SL1-2-R-2, SL1-2-R-3, SL1-2-R-4, SL2-2-R-15, and SL3-1-R-16. Amylolytic activity of the 17 themorphilic 'Actinobacteria' at various temperature was carried out using the starch agar plate method on Minimal (Mm) agar medium with the addition of 1% (w/v) soluble starch as substrate, and incubated at 45, 50, 55, and 60 ^oC for up to 7 days. Amylolytic activity was detected by flooding the plates with 1.5 ml of Lugol's iodine solution. Clear zones around the colonies indicated positive results for amylolytic activity. The results showed that most of the isolates obtained from the soil near the Cisolok geyser have varying amylolytic activity at a temperature of 45--60° C. In this study, 15, 13, four, and three out of 17 isolates were positive for amylolytic activity at 45, 50, 55, and 60 ^oC, respectively. Meanwhile, two isolates, designated SL2-2-R-15 and SL3-1-R-16, showed negative results for amylolytic activity at 45, 50, and 55 ^oC, even after 7 days of incubation. Three potential isolates which have amylolytic activity at 60 ^oC (designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4), based on 16S rRNA gene sequence, phylogenetic analysis, and phenotypic characterizations were identified as 'Actinomadura keratinilytica.'"

"Five kinds of endophytic fungi i.e pastalotiopsis sp. isola GNC fusarium sp, GNC -A fusarium sp..GNC-B.Pastalotiopsis sp, GUC and fusarium sp

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"Actinomycetes yang diisolasi dari habitat laut diketahui memiliki potensi sebagai penghasil enzim baru yang bermanfaat bagi industri. Kelimpahan keanekaragaman kelompok bakteri ini di Indonesia serta kurangnya penelitian mendorong kebutuhan akan informasi lebih mendalam. Isolat Actinomycetes (BLH 5-14) dari sedimen laut Sarena Kecil, kota Bitung, Sulawesi Utara, menunjukkan potensi sebagai penghasil enzim pektinase dan xilanase. Kedua enzim ini dibutuhkan dalam pengolahan jus buah, kertas, serat rami, dan produksi oligosakarida. Penelitian bertujuan untuk mengkarakterisasi enzim pektinase dan xilanase oleh isolat BLH 5-14 menggunakan substrat komersial. Hasil yang diperoleh menunjukkan bahwa enzim pektinase memiliki aktivitas tertinggi pada hari produksi ke-6 sebesar 4,72±0,08 U/mL dengan pH optimum 8,0 dan suhu optimum 50℃, sedangkan enzim xilanase memiliki aktivitas tertinggi pada hari produksi ke-6 sebesar 7,63±0,03 U/mL dengan pH optimum 6,0 dan suhu optimum 60℃. Inhibitor terbesar bagi kedua enzim merupakan Hg2+ dan SDS, sedangkan ion Fe2+ dapat meningkatkan aktivitas hingga dua kali lipat. Melalui tahapan hidrolisis dan TLC, diketahui pula bahwa enzim pektinase dan xilanase mampu menghasilkan monosakarida seperti galacturonic acid (P1), dan oligosakarida seperti xylotriose (X3), xylotetraose (X4), dan xylopentaose (X5), yang dengan pengembangan lebih lanjut dapat dimanfaatkan sebagai bahan prebiotik bagi industri kesehatan. Berdasarkan hasil sekuens 16S rDNA, isolat Actinomycetes (BLH 5-14) diidentifikasi sebagai genus Streptomyces dengan kemiripan terdekat Streptomyces tendae (99,78%).

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Actinomycetes isolated from marine habitats are known to have the potential for novel enzymes that are beneficial for the industry. The high diversity of the bacterial group in Indonesia and the lack of research that exist have prompted the need for more in-depth information. Actinomycetes isolates (BLH 5-14) that were obtained from marine sediments of Sarena Kecil, Bitung, North Sulawesi, showed potential to produce pectinase and xylanase needed in the processing of fruit juice industry, paper, ramie, and the production of oligosaccharides. This study aimed to characterize the pectinase and xylanase enzymes produced by BLH 5-14 isolates using commercial substrates. The results revealed that the pectinase had the highest activity on the 6th day (4.72±0.08 U/mL) at the optimum pH of 8.0 and optimum temperature of 50℃, while the xylanase had the highest activity on the 6th day (7.63±0.03 U/mL) at optimum pH 6.0 and optimum temperature 60℃. The most significant inhibitors for both enzymes are Hg2+ and SDS, while Fe2+ can increase enzyme activity up to two times the original value. Hydrolysis and thin layer chromatography also showed that pectinase and xylanase were able to produce oligosaccharides such as galacturonic acid (P1), xylotriose (X3), xylotetraose (X4), and xylopentaose (X5), which with further development can be used as prebiotics for the healthcare industry. Based on the results of 16S rDNA sequences, BLH 5-14 were identified as genus Streptomyces, with closely related species Streptomyces tendae (99,78%)."

"Rare-actinomycetes tersebar di berbagai habitat terutama di habitat ekstrem seperti kawasan geotermal. Penelitian mengenai rare-actinomycetes dilakukan terkait potensinya sebagai penghasil senyawa bioaktif baru yang bermanfaat dalam bidang kesehatan, industri dan farmasi. Tujuan dari penelitian ini adalah mengisolasi dan mengkarakterisasi secara fenotip dan genotip rare-actinomycetes dari sampel tanah di bawah batuan kuarsa (S3.5.3) di hutan kawasan geotermal Cisolok. Metode pengayaan sampel tanah dilakukan menggunakan medium 10% R2A (Reasoner’s 2A) cair dengan penambahan cycloheximide 100 ppm dan sodium azide 60 ppm, kemudian diinkubasi pada suhu 30°C selama 30 hari. Isolasi rare actinomycetes dilakukan dengan metode membran filter dan spread pada medium 10% R2A gellan gum yang diinkubasi pada suhu 45°C. Karakterisasi dilakukan secara genotipik (berdasarkan data sequence gen 16S rRNA, analisis homologi sequence, dan rekonstruksi pohon filogenetik dengan metode Neighbour-Joining, Maximum Likelihood, dan Minimum Evolution); dan karakterisasi fenotipik (morfologi, fisiologi, dan biokimia). Sebanyak 26 isolat diperoleh dari sampel S3.5.3. Lima isolat dengan karakter morfologi actinomycetes yang diisolasi dari suhu 45°C dengan membran filter, dipilih untuk diidentifikasi. Hasil analisis sequence gen 16S rRNA dari lima isolat menunjukkan persentase homologi sebesar 95,46-99,56% terhadap Micromonospora yasonensis DS3186T. Berdasarkan hasil analisis filogenetik dengan metode Neighbour-Joining, kelima isolat memiliki hubungan kekerabatan terdekat dengan Micromonospora yasonensis DS3186T. Kelima isolat merupakan anggota class Actinomycetes, ordo Micromonosporales, family Micromonosporaceae. Karakter fenotipik kelima isolat sesuai dengan Micromonospora yasonensis sebagai spesies terdekatnya. Kelima isolat merupakan bakteri termotoleran (tumbuh pada suhu 30-45°C dan suhu optimum 40°C), aerobik, Gram positif, menghasilkan miselium substrat tanpa adanya miselium aerial, positif katalase, dan menghasilkan soluble pigment. Penelitian ini mengungkapkan bahwa Micromonospora yasonensis dapat ditemukan di kawasan geotermal dan berasosiasi dengan batuan kuarsa.

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Rare-actinomycetes are distributed in various habitats, particularly in extreme environments such as geothermal areas. Research on rare-actinomycetes focuses on their potential as producers of new bioactive compounds beneficial in health, industrial, and pharmaceutical fields. The aims of this study were to isolate and characterize rare-actinomycetes from soil samples beneath quartz rocks (S3.5.3) in the geothermal forest of Cisolok. Soil sample enrichment was performed using 10% R2A (Reasoner’s 2A) liquid medium supplemented with 100 ppm cycloheximide and 60 ppm sodium azide, incubated at 30°C for 30 days. Rare actinomycetes isolation was carried out using the membrane filter method and spread on 10% R2A agar with gellan gum, incubated at 45°C. Characterization included genotypic analysis based on the sequence of 16S rRNA gene, supported by phenotypic characterization (morphology, physiology, and biochemistry). A total of 26 isolates were obtained from sample S3.5.3. Five isolates with actinomycetes morphology isolated at 45°C using the membrane filter method were selected for characterization. Analysis of the 16S rRNA gene sequences from these five isolates showed homology levels of 95.46-99.56% to Micromonospora yasonensis DS3186T. Based on phylogenetic analysis using the Neighbour-Joining method, the five isolates were most closely related to Micromonospora yasonensis DS3186T. These isolates belong to the class Actinomycetes, order Micromonosporales, and family Micromonosporaceae. Phenotypic characteristics of the five isolates were consistent with Micromonospora yasonensis as their closest species. These isolates are thermotolerant bacteria (growing at temperatures of 30-45°C; optimum temperature 40°C), aerobic, Gram-positive, produce substrate mycelium without aerial mycelium, positive for catalase, and produce soluble pigment. This study reveals that Micromonospora yasonensis can be found in geothermal areas associated with quartz rocks."