"
ABSTRAKPendahuluan: Sambiloto atau Andrographis panniculata merupakan sebuah
tanaman herbal yang memiliki khasiat sebagai antimalaria dengan cara
meningkatkan kerja antioksidan dalam tubuh. Hati merupakan salah satu tempat
terjadinya fase perkembangan Plasmodium pada penyakit malaria. Penelitian ini
bertujuan untuk menganalisis aktivitas antimalaria dari Ekstrak Etanol Sambiloto
(EES) pada mencit yang diiinfeksi Plasmodium berghei secara in vivo melalu
pengukuran kadar MDA dan aktivitas spesifik katalase jaringan hati.
Metode: Desain penelitian yang digunakan adalah eksperimental in vivo
menggunakan hewan coba mencit Balb/c. Metode penelitian dilakukan dengan
mengelompokkan mencit ke dalam empat kelompok yaitu kelompok kontrol yang
tidak diberi perlakuan, kelompok I yang diinduksi Plasmodium berghei tetapi
tidak diterapi, kelompok II yang diinduksi Plasmodium berghei dan diberi EES 2
mg/kgBB serta kelompok III yang diinduksi Plasmodium berghei dan diberi
klorokuin 10 mg/kgBB selama 3 hari. Analisis kadar MDA dilakukan dengan
metode Wills dan aktivitas spesifik katalase dengan metode Mates et al.
Hasil: Hasil penelitian menunjukkan terjadi penurunan kadar MDA yang tidak
signifikan pada mencit yang diinfeksi dengan Plasmodium berghei dan diberi
ekstrak etanol sambiloto (EES) 2 mg/kgBB dibandingkan dengan kontrol negatif
(66.49 ± 22,92 vs 69.40 ± 11,69 nmol/g jaringan hati). Namun pada kelompok
yang diberi perlakuan klorokuin juga terlihat penurunan kadar MDA yang tidak
signifikan dibandingkan dengan kontrol negatif (67.49 ± 7,04 vs 69.40 ± 11,69
nmol/g jaringan hati). Sedangkan aktivitas spesifik katalase kelompok yang diberi
EES menunjukkan peningkatan yang tidak berbeda bermakna dibandingkan
dengan kelompok kontrol (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg jaringan hati).
Begitupula dengan klorokuin yang menunjukkan peningkatan aktivitas spesifik
katalase yang tidak berbeda bermakna dibandingkan dengan kelompok kontrol
(2,97 ± 1,53 vs 3,73 ± 1.56).
Kesimpulan: Pada kelompok dengan pemberian EES 2 mg/kgBB terjadi
penurunan kadar MDA serta peningkatan aktivitas spesifik katalase jaringan hati
mencit dibandingkan dengan kelompok negatif, tetapi secara statistik tidak
bermakna demikian pula dengan kelompok yang diberi klorokuin.
ABSTRACTIntroduction: Andrographis panniculata or Sambiloto is a herbal plant that has
antimalarial efficacy by increasing antioxidant in body. Liver is one of the places
for Plasmodium to develop themselves in malaria. This research aims to analyze
the activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice which
infected by Plasmodium berghei in vivo through the measurement of MDA level
and the specific activity of catalase in liver tissue.
Method: We used experimental in vivo as the reserach design, using balb/c. The
research design is done by grouping the mices into four groups which of the
untreated group, group I-induced by Plasmodium berghei but not treated, group
II-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,
group III-induced Plasmodium berghei and treated with chloroquine with 10
mg/kg Body weight in three days. The MDA level analyze is done by the Wills
method and the specific activity of catalase with Mates et al method.
Result: The research result showed the decrease of MDA level which not
significant in mice that is infected by Plasmodium berghei and treated by SEE 2
mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/g
liver tissue). However, group that is infected by Plasmodium berghei and treated
by chloroquine also showed the decrease of MDA level which not significant
compared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).
Instead, group which treated by SEE showed the increase in specific activity of
catalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).
Similarly with chloroquine group which showed an increase in specific activity of
catalase were not significantly different compared with the control group (2.97 ±
1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).
Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decrease
of MDA level and also the increase of catalase specific activity in mice liver tissue
compared negative control, but statistically not significant as well as the group
given chloroquine;Introduction: Andrographis panniculata or Sambiloto is a herbal plant that has
antimalarial efficacy by increasing antioxidant in body. Liver is one of the places
for Plasmodium to develop themselves in malaria. This research aims to analyze
the activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice which
infected by Plasmodium berghei in vivo through the measurement of MDA level
and the specific activity of catalase in liver tissue.
Method: We used experimental in vivo as the reserach design, using balb/c. The
research design is done by grouping the mices into four groups which of the
untreated group, group I-induced by Plasmodium berghei but not treated, group
II-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,
group III-induced Plasmodium berghei and treated with chloroquine with 10
mg/kg Body weight in three days. The MDA level analyze is done by the Wills
method and the specific activity of catalase with Mates et al method.
Result: The research result showed the decrease of MDA level which not
significant in mice that is infected by Plasmodium berghei and treated by SEE 2
mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/g
liver tissue). However, group that is infected by Plasmodium berghei and treated
by chloroquine also showed the decrease of MDA level which not significant
compared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).
Instead, group which treated by SEE showed the increase in specific activity of
catalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).
Similarly with chloroquine group which showed an increase in specific activity of
catalase were not significantly different compared with the control group (2.97 ±
1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).
Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decrease
of MDA level and also the increase of catalase specific activity in mice liver tissue
compared negative control, but statistically not significant as well as the group
given chloroquine;Introduction: Andrographis panniculata or Sambiloto is a herbal plant that has
antimalarial efficacy by increasing antioxidant in body. Liver is one of the places
for Plasmodium to develop themselves in malaria. This research aims to analyze
the activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice which
infected by Plasmodium berghei in vivo through the measurement of MDA level
and the specific activity of catalase in liver tissue.
Method: We used experimental in vivo as the reserach design, using balb/c. The
research design is done by grouping the mices into four groups which of the
untreated group, group I-induced by Plasmodium berghei but not treated, group
II-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,
group III-induced Plasmodium berghei and treated with chloroquine with 10
mg/kg Body weight in three days. The MDA level analyze is done by the Wills
method and the specific activity of catalase with Mates et al method.
Result: The research result showed the decrease of MDA level which not
significant in mice that is infected by Plasmodium berghei and treated by SEE 2
mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/g
liver tissue). However, group that is infected by Plasmodium berghei and treated
by chloroquine also showed the decrease of MDA level which not significant
compared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).
Instead, group which treated by SEE showed the increase in specific activity of
catalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).
Similarly with chloroquine group which showed an increase in specific activity of
catalase were not significantly different compared with the control group (2.97 ±
1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).
Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decrease
of MDA level and also the increase of catalase specific activity in mice liver tissue
compared negative control, but statistically not significant as well as the group
given chloroquine"
Jakarta: Fakultas Kedokteraan Universitas Indonesia, 2013
