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"Penelitian uji klinik dengan one group pre-post test design bertujuan untuk mengetahui pengaruh pemberian susu bubuk kedelai terbadap peroksidasi lipid dengan mengukur kadar MDA. Terdapat 21 subyek perempuan perimenopause deugan hiperkolestero!emia yang memenuhi kriteria penelitian, mengkomsumsi susu bubuk kede!ai setiap hari sebanyak 2x30g selama delapan minggu, Data yang diambil adalah: data demografi, IMT, asupan zat gizi, isoflavon dan emioksidan. Data laboratorium meliputi kadar kolesterol LDL dan MDA serum sebelum dan sesudah empat, delapan minggu perlakuan. Uji statistik yang digunakan adalah uji t-berpasangan blla distribusi normal dan uji Wilcaxon bila distribusi tidak normal dengan tingkat kemaknaan p<0,0:5. Penelitian ini telah mendapat ijin dari Komite Etik FKUI. Dua subyek drop out, 19 subyek menyelesaikan penetitian; umumnya berlatar belakang pendidikan rendah rerata usia 49,15 tahun dan lMT tergolong berisiko. Asupan kalori subyek penelitian sebelum perlakuan tergolong kurang, tetapi kemudian tergolong cukup setelah perlalkuan. Pola dan asupan harian isollavon subyek penelitian sebelum perlakuan tergolong cukup, meningkat setelah perlakuan. Pola dan asupan harian antioksidan subyek sebelum dan selama masa perlakuan tergolong krang, Rerata kadar kolesterol LDL subyek penelitian sebelum masa perlakuan adalah 134,32 ± 23,70 mg/dl. Setelah perlakuan menurun, tetapi masih tergolong batas tinggi. Rerala kadar MDA serum subyek penelilian sebelum masa perlakuan adalah 0,82 ± 0,47 nmol/mL. Setelah empat dan delapan minggu masa perlakuan kadar MDA serum meningkat, yaitu masing­ masing sebesar 0,98 ± 0,26 umol/ml (p 0,16) dan 1,13 ± 0,40 nmol/ml (p 0,023). Beberapa faktor yang mungkin menjadi penyebab peningkatan tersebut adalah faktor subyek, biomarker MDA, bioaviabilitas dan karakteristik isoflavon serta asupan antioksidan. Bila subyek digolongkan berdasarksn status pre dan pasca menopause, maka setelah minggu IV pedakuan golongan premenopause menunjukkan penurunan kadar MDA yang lebih baik.

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The objective of this study is to investigate the effect of soy powder-milk supplementation on lipid peroxidation which is measured by the level of it's metabolite, malondialdehyde (MDA). Twenty one hypercholesterolentic pre-menopause women who fulfilled the study criteria. started ro consume 2x30g soy powder-milk everyday for eight week. Data taken were: demographic, anthropometric, nutrition intake, isoftavone, antioxidant pattern of isotlavone and antioxidant intake. Whilst laboratory data taken were level of LDL cholesterol and MDA serum before and after four and eight week supplementation. Statistical tests used were paired test if named distribution and Wilcoxon for up normal distribution with significance level of 5%.

Nineteen subjects completed the study. Most subjects had a low educational background, mean age were 49.15 years old and bed BMI classified as "risk." The subjects' calorie intake before supplementation was low, however after the forth and eighth week of supplementation was renegade as sufficient. Subjects' inkwell pattern and daily intake of isoflavoue were sufficient anti increased during supplementation. outtake pattern anti daily inkwell of antioxidant subjects before and doting supplementation were low. The subjects' mean level of LDL cholesterol before supplementation was 134,32 ± 23,70 mgldl. After four anti eight week supplementation it decrease considerably at 120,79 ± 21,30 and 122,68 ± 20,95 mgldL, which was still categorizeas "high". Subjects' mean level of MDA serum before supplementation was 0,82 ± 0,47 nmoVmL.

After fuur anti eight week of supplementation level of MDA serum was increase consecutively at 0,98 ± 0,26 mnollmL (p = 0,16) and 1,13 ± 0,40 nmollmL (p = 0,023). Several fuctJS that might cause the increase were subjects' age, menopausal status, and BMJ, MDA biomalker, bio availabilily and characteristics of isotlavoue and antioxidant intake. The g100ped of subjects in pre and post-menopause status sbowu different pattern of MDA level which is after four weeks of supplementation the pre-menopause subjects shown reduced of MDA level more than post menopausal subjects."

"ABSTRAKTujuan penelitian ini adalah untuk mengetahui efek pamberian bubuk susu kedelai2x30 gramlbari, selama 8 minggu terhadap kadarkolesterol LDL dan HDL serum pada wanitaparimenopauseBertempat di Klinik Seruni Departemen Ilmu Gizi Fakultas KedokteranUniversitas lndnesia, jalan Salemba Raya no.6, JakartaPenelitian dengan rancangan one group pre-post tes yangtelah disetujui Komite Etik FKUI. Subyek mendapatsuplementasi susu bubuk kedelai 2x30 g/hari selama 8minggu. Pada awal (minggu 0); pertengahan (minggu IV);dan akhir penelitian ( awal minggu IX) dilakukanpemeriksaan kadar kolesterol LDL dan HDL. Data isilpanzat gizi dilukukan sebelum dan selama penelitianberlangsung, dengan menggnnakan food recall l x24 jam.Uji statistik yang digunakan adalah uji t berpasangan jikadata berdistnbusi normal dan Wilcoxon, jika databerdistribusi tidak normal. Tingkat kemaknaan yangdigunakan p<0,05.Sebanyak 19 subyek penelitian yang dapat mengikutipenelitian sampai selesai. Setelah delapan mingguperlakuan, didapatkan adanya penurunan yang bermakna(p<0,05) pada kadar kole-ol LDL, yaitu sebesar8,59±17,31% di minggu IV dan 7,81±11,32% di minggu IX.Kadar kolesterol HDL menurun pada minggu IV dan IX,namun tidak bermakna (p>0,05). Rasio kolesterol LDLterhadap HDL, men= secilla bermakna (p<0,05) diminggu IV, yaitu sebesar 7,03±16,82%, sedangkan diminggu IX terjadi penurunan, namun tidak bermakna(p>0,05), sebesar 4,04±12,25%.Pemberian bubuk susu kedelai 2x30 g/hari selama delapanminggu, dapat menurunkan kadar kolesterol LDL sertarasio LDLIHDL secara bermakna.

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AbstractThis research aim to investigate the effects of2x30 lifd soy milk flour, for eight. weeks on serumo cholesterol LDL andHDL levels inhypercholesterolemic perimenopausal womenLocated at Seruni Clinic, Depar1ment of Nutrition, Faculty of MedicineUniversity of IndonesiaThe study was a one group pre-post test design, which wasapproved by The Ethical Clearance Research Committee ofFaculty of Medicine University of Indonesia. The subjectsreceived 2x30 g/d soy milk powder for eight weeks. Serum LDLand HDL cholesterol levels were determined at the heghming(week 0), the middle (week4), and the end of the stndy(earlyweek 9). Dietery intakes were assessed using lx24 hours foodrecall. Statistical analysis was performed using dependent t tesfor normal distribution and Wilcoxon for not normal distributiondata. The level of significancy was 5% (p<0,05)There were 19 subjects who completed the study. After eightweeks intervention, there was 8.59±17.31% significant decreasedin LDL cholesterol levels at the 4th week of the study and7.81±1 1.32% at the s"' week of the study (p<0,05).HDLcholesterol levels decreased at the 4\h and 8" weeks, but notsignificant(p>0,05). The ratio ofLDL to HDL was 7,03±16,82o/o,which was significant decreased at the 4" week (p<0,05), while atthe 9th week the decrement was not significant (4.04±12.25%),p> 0.05.Consuming soy milk powder 2 x 30 g/dduring eight weeks, canreduce the LDL cholesterol level and LDLIIIDL ratiosignificantly."

"Mengetahui pcngaruh pemberian kombinasi suplementasi vitamin E dan C terhadap peroksidasi lipid pada usila dengan hiperkolesterolemia. Penelitian uji klinis paralel, tertutup timggai, alokasi acak, untuk membandingkan kadar malondialdehida usila 2,60 tahun dengan hiperkolesterolemia yang mendapatkan kombinasi supiementasi vitamin E 400 IU dan vitamin C 500 mg, masing~masing sebutir sehari selama 45 hari dengan kelompok yang mendapat vitamin E 400 IU dan plascbo.Terdapat 42 subyek penelitian yang berasal dari Yayasan Kebagusan, Yayasan Yasni, dan Yayasan Yakin, Pasar Minggu Jakarta Selatan yang dibagi menjadi dua kelompok masing-masing berjumlah 21 orang. Data yang diambil adalah : data dcmograti, antropometzi, data asupan makanan pada minggu pertama, ketiga dan ketujuh, kadar kolesterol LDL dan MDA plasma sebelum dan sesudah perlakuan. Uji statistik yang digunakan adalah uji t-tidak bezpasangan bila distribusi nonnal dan uji Manmwhimey bila distribusi tidak normal dengan tingkat kemaknaan p<0.05. Sebanyak 20 subyek penelitian dad masing-masing kelompok yang dapat mengikuti penelitian sampai sclesai. Sebelum perlakuan, nilai median kadar kolesterol LDL kelompolc vitamin E+plasebo dan vitamin E+C masing- masing adalah I46.50(l30-190) mg/dL dan 146.50(l3I-196) mg/dL. Setelah 45 hari perlakuan, rerata kadar kolesterol LDL kelompok vitamin E4-plasebo (151.9.+:2.2.l mg/dl.) meningkat sedangkan kelompok vitamin B+-C (l46.8i28.21 mg/dL) menurun. Sebelum p¢rIakuan, nilai median kadar MDA plasma kelornpok vitamin E+plasebo dan rerata kadar MDA plasma kelompok vitamin E4-C masing-masing adalah 2.63(l.92-4.42) nmol/ml., dan 3.03:l:0.62 nmol/mL. Setelah 45 haii pcrlakuan rerata kadar MDA plasma kedua keiompok menunm menjadi 2.30i0.67 nmol/mL (p<0.01) pada kelompok vitamin E+plasebo dan 28810.88 nmol/mL (p=0.36) untuk kelompok vitamin E+C. Penurunan kadar MDA plasma kelompok vitamin E+plasebo lcbih besar (-0.5:!:0.55 nmol/mL) daripada kelompok vitamin E+C (-0.28(l.31-1.63) nmol/mL), tetapi dcngan uji statislik terhadap kedua nilai tersebut, tidalc berbeda bcrma!ma(p=0.09). Pembenan kombinasi vitamin E dan vitamin C pada usila dengan hiperkolesternlemia tidak dapat rnenurunkan kadar MDA plasma lcbih besar dibandingl-can dengan hanya pemberian vitamin E.

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This parallel, single blind, randomization clinical trial purpose was to compare plasma malondyaldehydc level in hypercholesterolemic elderly aged more than 60 years old. Forty two people from Yayasan Kebagusan, Yayasan Yasni and Yayasan Yakin, Pasar Minggu, South Jakarta which participated the study, were divided into two groups. Twenty one elderly were supplemented with 400 IU vitamin E and 500 mg vitamin C for 45 consecutive days, while the other group was supplemented with 400 IU vitamin E and placebo. The data of demographic, anthropometric, food intake in the first, third and seventh weeks, plasma LDL and MDA levels before and alter period were taken. Statistical analyzes was performed by SPSS 11.5.

Twenty people for each group had followed the study until the end of period. Before study, LDL cholesterol median for vitamin E + placebo group and vitamin E+C group were 146.50(l30-190) mg/dL and l46.50( 130-190) mg/dL respectively. Alter 45 of days treatment, there was an increase in mean LDL cholesterol in vitamin E + placebo group 15l.9i22.1 mg/dL while in vitamin E+C group was decreased to l46.8:l:28.2l mg/dl Before study, plasma MDA level in vitamin E + placebo group and vitamin E+C group were 2.63(l.92-4.42) and 3.031052 nmol/mL, respectively. After 45 days, mean MDA plasma in vitamin E + placebo group was 2.30i0.67 nmol/mL (p<0.01) and was 2.881088 nmol/ml.. (p=0.36) in vitamin E+C group. The decreased on plasma MDA levels in vitamin E+placebo group was higher (-0,510.55 nmol/mL) than vitamin E+C (-0.28(1.3l-1.63) nmol/mL), but statistical test showed not significant different between both group (p=0.09). Combined supplementation vitamin E and vitamin C in hypercholesterolemic elderly couldnot decrease plasma MDA higher than supplementation of vitamin E alone."

"Tujuan: Mengetahui pengaruh suplementasi formula tempo terhadap kadar malondialdehida dan F2-Isoprostan serum pada penderita ipedcolesterolemia. Tempat: P.T. National Gobel, Jakarta. Bahan dan cara: Penelitian eksperimental pada dua kelompok. Kelompok perlakuan dan kontrol masing-masing 17 subyek yang telah memenuhi kriteria penerimaan dan penolakan diberikan formula tempo (100 gram) per oral, sekali sehari selama 42 hari. Data yang dikumpulkan meliputi data rasio demografi, data nutrisi, data antropometri, data laboratorium, yaitu: malondialdehida (MDA) dan F2-Isoprostan serum. Hasil : Nilai median MDA serum kelompok perlakuan lebih rendah dibandingkan kelompok kontrol yaitu 0,88 (0,44 - 3,04) nmol/mL vs 1,07(0,39 - 2,02) nmol/mL. Narnun uji statistik terhadap perbedaan ini tidak bermakna. Nilai median F2 Isoprostan serum kelompok perlakuan 793,0 (100,9 - 6316,25) pg/mL lebih tinggi dibandingkan nilai median kelompok kontrol 391,75 (100,9 - 10172,5) pg/mL dengan analisis staliatik tidak berbeda bermakna. Pada kelompok perlakuan subyek perokok didapatkan korelasi kuat antara kadar MDA dan F2-lsoprostan serum, sedangkan pada kelompok kontrol tidak didapatkan korelasi bermakna. Keshnpulan: Pengaruh suplementasi formula tempe yang mengandung isofavon terhadap penurunan kadar MDA dan F2-Isoprostan serum belum dapat dibuktikan walaupun terlihat kecenderungan penurunan rentangan kadar MDA serum setelah suplementasi formula tempe selama 42 hari.

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Objective: To identify the effect of tempe formulae supplementation on the level of serum malondialdehide and F2-Isoprostane of the hypercholesterolemic subjects.

Place: P .T. National Gabel, Jakarta.

Materials and methods: An experimental study of two groups was carried out 17 subject's of treatment and control group fulfilled the criteria of the selection. The subjects were given 100 gram of tempe formulae orally, once a day for 42 days. The data collected were socio-demographic, nutritional, anthropometric and laboratory data such as: malondialdehide (MDA) and F2- Isoprostane.

Results: The median value of the serum MDA for the treatment group lower than the control group 0,88(0,44 - 3,04) nmol/mL vs 1,07(0,39 - 2,02) nmol/mL although statistical tests concerning this difference are not significance. The median value of serum F2-Isoprostane was 793,0 (100,9 - 6316,25) pg/mL for the treatment group higher compared to median value of 391,75 (100,9 - 10172,5) pg/mL for the control group. According to statistical analysis this difference was not significant. Subjetcs in the treatment group who were smokers showed a strong correlation between the level of MDA and F2-Isoprostane, whereas the control group did not show any significant correlation.

Conclusion: The effect of tempe formulae supplementation containing isoflavon on lowering the level of serum MDA and F2-lsoprostane have not been proven, although a tendency towards a decrease in the level of serum MDA was observed after 42 days supplementation of tempe formulae."

"Uji klinis paralel alokasi acak tersamar ganda ini bertujuan untuk mengetahui pengaruh pemberian kreatin monohidrat sebesar 20 gram/hari selama 7 hari berturut-turut terhadap kadar malondialdehida (MDA) plasma pasca latihan lari sprint pada atlet laki-laki lari jarak pendek (100 dan 200 meter), usia 18-25 tahun. Sejumlah 20 subyek dipilih dan dibagi menjadi dua kelompok dengan randomisasi blok, 10 subyek kelompok perlakuan (KP) dan 10 subyek kelompok kontrol (KK). Subyek KP mendapat kreatin monohidrat 20 gram/hari + maltodekstrin 50 gram/hari, sedangkan subyek KK mendapat maltodekstrin 50 gram/hari. Data yang diambil meliputi usia, indeks massa tubuh (IMT), massa lemak (ML), massa bebas lemak (MBL), cairan tubuh total (CTT), asupan energi, karbohidrat, protein, kreatin, karotenoid, vitamin C, vitamin E, dan kadar MDA plasma. Pemeriksaan kadar MDA plasma dilakukan sebelum dan setelah periode perlakuan. Analisis data menggunakan uji t tidak berpasangan dan uji Mann-Whitney dengan batas kemaknaan 5%. Analisis lengkap dilakukan pada 20 subyek yaitu 10 subyek KP [usia 18,50 (18,00-19,00 tahun)] dan 10 subyek KK [usia 18,00 (18,00-24,00 tahun)]. Kadar MDA plasma sebelum perlakuan pada KP dan KK adalah 0,32 ± 0,11 μM dan 0,33 ± 0,10 μM (p = 0,95). Kadar MDA plasma setelah perlakuan lebih rendah pada KP dibandingkan KK, yaitu KP 0,32 ± 0,11 μM dan KK 0,34 ± 0,13 μM (p = 0,66). Perbedaan perubahan kadar MDA plasma pada KP 0,00 ± 0,16 μM dan KK 0,01 ± 0,17 μM (p = 0,83). Tidak terdapat perbedaan signifikan perubahan kadar MDA plasma setelah pemberian kreatin monohidrat 20 gram/hari pada KP dibandingkan KK. Penelitian ini belum dapat membuktikan pengaruh pemberian kreatin monohidrat 20 gram/hari selama 7 hari berturut-turut dalam menurunkan kadar MDA plasma pasca latihan lari sprint pada atlet laki-laki lari jarak pendek.

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This parallel double-blind randomized clinical trial aims to investigate the effect of 20 gram/day creatine monohydrate supplementation for 7 days on plasma malondialdehyde (MDA) level after sprint running in male short-distance runner (100 and 200 meter) aged 18-25 years. A total of 20 subjects were selected and randomly allocated to one of two groups using block randomization, 10 subjects for treatment group (TG) and 10 subjects for control group (CG). The TG received 20 gram/day creatine monohydrate + maltodextrin 50 gram/day, and the CG received 50 gram/day maltodextrin. Data were collected in this study included age, body mass index (BMI), fat mass (FM), fat free mass (FFM), total body water (TBW), intake of energy, carbohydrate, protein, creatine, carotenoid, vitamin C, vitamin E, and plasma MDA level. Assessment of plasma MDA level was carried out before and after supplementation.

Statistical analyses included independent t-test and Mann-Whitney test with significance level was 5%. Twenty subjects completed this study, 10 subjects in TG [aged 18.50 (18.00-19.00) years] and 10 subjects in CG [aged 18.00 (18.00-24.00) years]. Plasma MDA levels before treatment were 0.32 ± 0.11 μM for TG and 0.33 ± 0.10 μM for CG (p = 0.95), respectively plasma MDA levels after treatment for TG was lower than CG; 0.32 ± 0.11 μM and 0.34 ± 0.13 μM (p = 0.66). The difference of plasma MDA level for TG was 0.00 ± 0.16 μM and CG was 0.01 ± 0.17 μM (p = 0.83). No statistically significant difference was found after 20 gram/day creatine monohydrate supplementation between 2 groups. This study has not proven yet the effect of 20 gram/day creatine monohydrate for 7 days in decreasing plasma MDA level after sprint running in male short-distance runner."

"Penyakit ginjal kronik merupakan kerusakan fungsi ginjal yang ireversibel. Stres oksidatif merupakan salah satu faktor utama dalam perkembangan penyakit ginjal kronik. Terdapat beberapa agen terapeutik yang dapat digunakan untuk menekan stres oksidatif, diantaranya adalah antioksidan. Beberapa herbal telah menunjukkan efek antioksidan dan dapat digunakan sebagai agen terapeutik. Tujuan penelitian ini adalah untuk menguji efikasi pemberian kombinasi Acalypha indica Linn pada dosis 200mg∙kgBB-1 dan Centella asiatica pada dosis 150mg∙kgBB-1 selama 3 hari, 7 hari, dan 14 hari untuk menekan stres oksidatif pada ginjal tikus Sprague dawley yang telah mengalami kondisi hipoksik selama 7 hari. Efek dari herbal ini akan dibandingkan dengan plasebo dan Pirasetam dengan dosis 50mg∙kgBB-1 selama 7 hari, serta tikus yang tidak mengalami hipoksia. Kadar malondialdehida (MDA) pada ginjal tikus tersebut digunakan untuk mengukur tingkat peroksidasi lipid. Hasil menunjukkan bahwa pemberian herbal ini menunjukkan perbedaan bermakna (P<0.05) antara tikus yang diberikan kombinasi herbal ini selama 7 hari dengan tikus yang tidak mengalami hipoksia, tetapi tidak terdapat perbedaan bermakna antara kelompok-kelompok tikus lain. Disimpulkan bahwa kombinasi Acalypha indica Linn dan Centella asiatica tidak mampu menurunkan kerusakan akibat peroksidasi lipid.

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Chronic kidney disease is the irreversible damage on kidney function. Oxidative stress is a major factor on the progression of chronic kidney disease. A number of therapeutic agents could be used to supress oxidative stress, such as antioxidants. Some herbals have been shown to have antioxidant property and could be used as therapeutic agents. The aim of this study was to test the efficacy of a combined supplementation of Acalypha indica Linn at 200mg∙kgBW-1 and Centella asiatica at 150mg∙kgBW-1 for 3 days, 7 days, and 14 days to supress oxidative stress in the kidneys of Sprague dawley mice that have been exposed to hypoxic condition for 7 days.

The effect of these herbals was compared to placebo and Piracetam at 50mg∙kgBW-1 for 7 days, as well as mice not exposed to hypoxic condition. Malondialdehyde (MDA) concentration on the kidneys of these mice was used to measure the extent of lipid peroxidation. The result showed that supplementation of these herbals caused a significant difference (P<0.05) between mice given the herbals for 7 days vs mice not exposed to hypoxic condition, but no other significant difference is found among the mice. It is concluded that combined supplementation of Acalypha indica Linn and Centella asiatica did not manage to reduce lipid peroxidation."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi larutan CMC 0,5%; KK 2 yang diberi larutan CMC 0,5% dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi larutan CMC 0,5% dan susu kedelai dengan fortifikan Fe fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah pencekokan hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah (P < 0,05) menunjukkan pengaruh nyata pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 27,90% terhadap KK 1 dan 17,49% terhadap KK 2.

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The effect of Fe fumarate fortificant addition in soy milk intake on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups, consist of normal control group (KK 1) which was administered with CMC 0.5% solution, treatment control group (KK 2) which was administered with CMC 0.5% solution and unfortified soy milk, and three treatment groups which were administered with soy milk added with fortificant Fe fumarate 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for consecutive 21 days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post-hoc LSD test (P < 0.05) showed significant effect of fortificant Fe fumarate addition in soy milk intake on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 1, which is 27.90% to KK 1 and 17.49% to KK 2."

"ABSTRAKPenelitian ini bertujuan untuk membandingkan pengaruh pemberian teh putih dengan teh hijau pasca konsumsi makanan tinggi lemak terhadap perubahan kadar malondialdehida MDA plasma pada subjek hipertrigliseridemia borderline. Penelitian ini merupakan penelitian eksperimental dengan desain cross over, alokasi acak, tersamar ganda. Subjek yang diteliti sebanyak 18 orang adalah subjek pekerja kantor dengan hipertrigliseridemia borderline berusia 20-55 tahun di tiga perusahaan di Jakarta. Intervensi yang diberikan berupa minuman dengan 7,5 gram teh putih dan 7,5 gram teh hijau, masing-masing dalam 200 ml air dengan suhu 95-100oC dan penyeduhan selama 10 menit. Minuman teh putih maupun teh hijau dikonsumsi setelah subjek mengonsumsi makanan tinggi lemak. Kadar MDA plasma diukur sebelum dan 4 jam setelah intervensi. Usia rata-rata subjek adalah 33,94 tahun dengan mayoritas berjenis kelamin laki-laki dan memiliki status gizi obesitas I. Proporsi asupan lemak harian subjek melebih batas jumlah konsumsi yang dianjurkan yaitu 34 . Asupan karotenoid, vitamin C dan vitamin E subjek lebih rendah dari jumlah yang direkomendasikan.Terdapat perbedaan yang bermakna pada perubahan kadar MDA plasma 4 jam setelah konsumsi makanan tinggi lemak pada pemberian 7,5 gram teh putih dalam 200 mL air dibandingkan dengan pemberian teh hijau p=0,01 .

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ABSTRACTAbstract The objective of this study was to compare the effect of white tea to green tea after high fat food consumption on malondialdehyde MDA plasma level in borderline hypertriglyceridemia subjects. This is a randomized, experimental, cross over design with random allocation, double blind trial. There are 18 subjects consist of 14 male office workers ranging from 20 55 years old with borderline hypertriglyceridemia from three companies. Intervention is provided in the form of 7.5 grams green tea and 7.5 grams white tea each in 200 ml of water with a temperature of 95 100 C and brewed after 10 minutes. White tea and green tea were given after subjects consumed high fat food. Blood samples were collected pre meal and 4 hours post meal and assayed for MDA. The results showed that 10 out of 18 people were obese type I. The proportion of subjects daily fat intake were 34 which exceeds the limit of consumption recommended. On the contrary, the intake of carotenoids, vitamin C and vitamin E subjects are lower than the amount recommended. There were significant differences in the changes of MDA plasma level four hours after the consumption of high fat food in the supplementation of white tea compared to green tea p 0.01 . "

"Wanita Indonesia yang memasuki masa menopause cenderung mengalami obesitas, Gejolak panas sebagai salah satu gejala menopause yang paling sering dikeluhkan oleh wanita yang memasuki masa menopause, berkaitan dengan obesitas. Obesitas diketahui berhubungan dengan leptin, suatu hormon polipeptida yang mempunyai peran dalam reproduksi dan pusat pengatur suhu. Penelitian ini bertujuan mengetahui apakah terdapat peningkatan kadar leptin pada wanita perimenopause-menopause dengan gejolak panas di RSCM. Penelitian deskriptif dengan desain kasus-kontrol. Subjek penelitian 50 wanita perimenopause-menopause, berusia 40-55 tahun. Kelompok kasus dan kontrol ditegakkan berdasarkan Kuesioner Menqol Menopause. Kelompok gejolak panas adalah wanita yang menjawab YA pada pertanyaan Kuesianer Menqol Menopause 1-3, dengan kelompok kontrol adalah wanita yang tidak ada keluhan gejolak panas, dan menjawab tidak atau satu saja jawaban ya pada poin 1-3. Hasil: Tidak terdapat perbedaan yang bermakna antara kadar leptin pada kelompok yang mengalami gejolak panas dan kelompok tanpa gejolak panas dengan median leptin kasus vs control 21.86 (7.41-46.66) vs 16.53 (4.32-37.81) ng/ml , p=0.154. Meski demikian, terdapat hubungan yang signifikan antara obesitas dengan gejolak panas (p=0.047). Karakteristik gejolak panas yang didapatkan dikategorikan masih ringan karena frekuensi terjadinya gejolak panas yang jarang, dengan durasinya sangat cepat dan tidak mengganggu aktifitas. Gejolak panas cenderung dirasakan pada wanita berpendidikan menengah dibandingkan pendidikan tinggi (p=0.01), pada kelompok menengah ke atas (p 0.037),dan pada kelompok yang terbiasa tidur dengan air conditioner (p=0.057) dan berolahraga secara teratur (p 0.248). Kebiasaan mengkonsumsi tahu, tempe dan tidur cukup 6 jam sehari tidak memiliki hubungan terhadap terjadinya gejolak panas. Skor MENQOL Menopause kelompok dengan gejolak panas lebih tinggi dibandingkan dengan kelompok tanpa gejolak panas (p<0.001), serta gangguan kualitas hidup cenderung dirasakan lebih berat pada kelompok wanita dengan obesitas (p=0.061). Obesitas berhubungan bermakna dengan gejolak panas, tetapi leptin tidak berperan terhadap terjadinya gejolak panas. Gangguan kualitas hidup akibat gejolak panas cenderung dirasakan oleh kelompok yang obesitas.

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Hot flashes as one of menopausal symptoms that manifested to quality of life. Obesity has been linked to increased risk of hot flashes in menopausal women. Leptin as anti obesity hormone, has play a role in thermoregulatory dysfunction in menopause women with hot flashes. This research want to explore effect of leptin serum level to hot flashes in perimenopausal and menopausal women in Ciptomangunkusumo General Hospital. A descriptive study with case control design. The subject is 50 perimenopause women aged 45-55 years. The case and control groups are based MENQOL Questionnaire. Hot flashes are a group of women who answered YES to the question number 1-3, and control group of women who are no complaints of hot flashes. We performed bivariate analysis, using statistic by SPSS 17.

There is no significant differences between Leptin serum level in hot flashes group and non hot flashes with median level of leptin serum 21.86 (7.41-46.66) ng/ml, vs16.53 (4.32-37.81) ng/ml with p = 0.154. Obesity is correlated with hotflashes (p=0.047). Characteristics of hot flashes categorized as mild. The frequency of occurrence is rare, with very fast duration, and not disturb activity. To strata education obtained a meaningful correlation, hot flashes tending perceived in women with middle educated compared higher education (p=0.01), on group upper middle class than middle class (p 0.037) and women sleep with the air conditioner (p0.057) and exercise regularly (p=0.248). The habit of eating soy product such as tofu and tempe and slept 6 hours a day, not correlated statistically with the the occurrence of hot flashes. There is a significant difference in score of MENQOL Menopause, whereas in the group with hot flashes compared to without hot flashes. (p<0.001), impaired quality of life tend to be felt more severely in the group of women with obesity (p0.061). Obesity is statistically correlated with hotflashes, and leptin. But elevated leptin serum didn?t statistically correlated with hotflashes. Quality of life disturbance is severe in obesity group. Obesity can causes hot flashes with other mechanism such as fat as heat insulator."

"ABSTRAKTelah dilakukan penelitian untuk mengetahui pengaruh pemberian fortifikanNaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadar natrium plasmadarah tikus (Rattus norvegicus L.) jantan galur Sprague Dawley. Sebanyak 25ekor tikus dibagi ke dalam 5 kelompok: kelompok kontrol 1 (KK1) yang diberiCMC 0,5%, kelompok kontrol 2 (KK2) yang diberi tepung tempe atau susukedelai, dan tiga kelompok perlakuan (KP1, KP2, KP3) yang diberi tepung tempeatau susu kedelai dengan fortifikan NaFeEDTA dosis 1,35 mg Fe/ kgBB; 2,7 mgFe/ kgBB; 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Penentuan kadarnatrium plasma dengan alat AES (Atomic Emission Spectroscopy). Hasil ujiAnava satu arah (P > 0,05) menunjukkan tidak ada pengaruh nyata pemberianfortifikan NaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadarnatrium antar kelompok perlakuan. Kadar natrium plasma pada T21 dengan bahanuji tepung tempe dan susu kedelai tetap berada pada rentang normal antara 0,456mg/ml -- 0,586 mg/ml.

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ABSTRACTThe effect of NaFeEDTA fortificant inserted in tempeh flour and soy milk intakeon plasma sodium concentration in male Sprague-Dawley rats (Rattus norvegicusL.) had been studied. Twenty five rats were divided into five groups: controlgroup 1 (KK1) was administered with CMC 0.5%, control group 2 (KK2) wasadministered with tempeh flour or soy milk; three treatment groups (KP1, KP2,KP3) were administered with tempeh flour or soy milk added with fortificantNaFeEDTA 1.35 mg Fe/kgBw; 2.7 mg Fe/kgBw; 5.4 mg Fe/kgBw consecutivefor 21 days. Plasma sodium concentration was measured by AES (AtomicEmission Spectroscopy). One way Anova test (P > 0.05) showed there is nosignificant effect of fortificant NaFeEDTA inserted in tempeh flour and soy milkintake on plasma sodium concentration in all treatment groups. Plasma sodiumconcentration on T21 which was administered with tempeh flour and soy milkremains in normal range between 0.456 mg/ml ? 0.586 mg/ml."