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"Lovastatin dapat berfungsi menghambat proses pembentukan kolesterol dalam hati, dengan cara penghambatan secara kompetitif terhadap sintesis enzim 3 hydroxy -3-methylglutaryl-koenzim A reduktase (HMG-KoA reduktase). Penelitian bertujuan untuk melakukan skrining kapang Aspergillus. spp. koleksi University of Indonesia Culture Collection (UICC), fermentasi, dan analisis produksi lovastatin. Hasil skrining lovastatin dari sebanyak 40 biakan Aspergillus spp. koleksi UICC 18 biakan (45%) positif menghasilkan lovastatin dan 22 biakan (55%) negatif. Aspergillus flavus UICC 360 menghasilkan lovastatin tertinggi dibandingkan sepuluh Aspergillus spp. terpilih. Analisis Thin Layer Chromatografy (TLC) menunjukkan nilai Rf sepuluh Aspergillus spp. hampir sama dengan standart. Analisis High Performance Chromatography (HPLC) mengkonfirmasi lovastatin Asp. flavus UICC 360 mempunyai waktu retensi sama dengan standart (13,2 menit).

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Lovastatin is an acting inhibitor of enzyme 3 hydroxy -3-methylglutaryl-coenzyme A reduktase (HMG-CoA reductase) as competitive inhibitor on biosynthesis of cholesterol. The aims of the research is for the selection, fermentation, and analysis of lovastatin from isolates mold Aspergillus spp. at the University of Indonesia Culture Collection (UICC). The results revealed that out of 40 cultures, 18 cultures (45%) produced lovastatin and 22 cultures (55%) were negative. Aspergillus flavus UICC 360 showed the highest lovastatin production compared of 10 selected cultures. Thin Layer Chromatografy (TLC) analysis amount of 10 Aspergillus spp. have similarity of Rf value compared to the standard. High Performance Chromatography (HPLC) analysis confirmed that lovastatin Aspergillus flavus UICC 360 has the same retention time with the standard (13.2 minutes)."

"ABSTRAKPenelitian ini bertujuan untuk melakukan skrining kapang Aspergillus spp.koleksi University of Indonesia Culture Collection (UICC), fermentasi dananalisis produksi antioksidan. Hasil skrining antioksidan dari 12 biakanAspergillus spp. menggunakan metode fitokimia mengandung senyawaantioksidan: flavonoid, triterpenoid, alkaloid dan glikosida. AnalisisKromatografi Lapis Tipis (KLT) menggunakan BHT sebagai standart dari 12Aspergillus spp. menunjukkan Aspergillus awamori UICC 9 memiliki nilai Rfyang hampir sama dengan standart. Analisis DPPH (2,2 diphenil-1-pikrihidrazil)mengkonfirmasi antioksidan Aspergillus awamori UICC 9 memiliki EC 50 sebesar1359.5 ppm

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AbstractThe aim of this research is to screen Aspergillus spp molds, that belongedto the University of Indonesia Culture?s Collection (UICC) for antioxidantproduction. The result of phytochemical analysis process of 12 Aspergillus spp.consist of flavonoid, triterpenoid, alkaloid and glycoside. The Thin LayerChromatography (TLC) using BHT as standart to analyse of 12 Aspergillus spp.show that Rf value of Aspergillus awamori UICC 9 has Rf value is same asstandard (0.16). An antioxidant activity using DPPH (2,2 diphenil-1-pikrihidrazil)analysis confirmed that Aspergillus awamori UICC 9 has EC50 is 1359.5 ppm"

"Telah dilakukan penelitian yang bertujuan mengetahui pengaruh fortifikan NaFeEDTA dalam susu kedelai terhadap kadar hemoglobin darah tikus Rattus norvegicus L. jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap RAL . Sebanyak 25 ekor tikus putih jantan dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar tanpa pemberian susu kedelai dan fortifikan; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA dosis 2,7 mgFe/ kgBB; 5,4 mgFe/ kg BB; dan 10,8 mgFe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Pengukuran kadar hemoglobin darah tikus menggunakan hematology analyzer. Hasil uji ANAVA satu arah dan uji LSD P < 0,05 menunjukkan bahwa setelah pemberian perlakuan selama 21 hari berturut-turut, terdapat perbedaan kadar hemoglobin yang nyata antara seluruh kelompok perlakuan KP 1, KP 2 dan KP 3 terhadap kadar hemoglobin KK 1 dan perbedaan kadar hemoglobin yang nyata antara KP 2 dan KP 3 terhadap kadar hemoglobin KK 2. Peningkatan kadar hemoglobin tertinggi terjadi pada KP 2, yaitu 10,84 terhadap KK 1; dan 9,28 terhadap KK 2.

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The effect of NaFeEDTA fortificant in soymilk on blood hemoglobin levels of male Sprague Dawley rats Rattus norvegicus L. had been studied. By using Complete Random Design CRD , twenty five rats were divided into five groups. Normal control group KK 1 was administered with standard feeding and drinking without adding soymilk and fortificant. Treatment control group KK 2 was administered with soymilk without fortificant, and three treatment groups which were administered with soymilk added with NaFeEDTA fortificant 2.7 mg Fe kgbw KP 1 5.4 mg Fe kgbw KP 2 and 10.8 mg Fe kgbw KP 3 . All of the five groups were treated for 21 days consecutively. Measurement of rat blood hemoglobin levels uses the hematology analyzer. One way ANOVA test and post hoc LSD test P 0.05 showed that after 21 days of consecutive treatment, there was a significant effect on blood hemoglobin levels in all treatment groups KP 1, KP 2, and KP 3 compared to KP 1 and a significant effect on blood hemoglobin levels in KP 2 and KP 3 compared to KK 2. The highest increase of blood hemoglobin levels was detected on KP 2 which is 10.84 to KK 1 and 9.28 to KK 2."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi larutan CMC 0,5%; KK 2 yang diberi CMC 0,5% dan suspensi tepung tempe tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi CMC 0,5% dan tepung tempe dengan fortifikan Fe Fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah pencekokan hari ke-21. Darah ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji Anava satu arah (P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 13,21% terhadap KK 1 dan 11,48% terhadap KK 2.

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The effect of Fe fumarate fortificant addition in tempeh flour on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups, consist of normal control group (KK 1) which was administered with CMC 0.5%, treatment control group (KK 2) which was administered with CMC 0.5% and tempeh flour non fortificant, and three treatment groups which were administered with tempeh flour added with Fe Fumarate fortificant 1.35 mg Fe/KgBW (KP 1); 2.7 mg Fe/KgBW (KP 2); and 5.4 mg Fe/KgBW (KP 3). All of the five groups were treated within 21 consecutive days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test (P < 0.05) showed significant effect of Fe Fumarate fortificant addition in tempeh flour intake on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 1 which is 13.21% to KK 1 and 11.48% to KK 2."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi larutan CMC 0,5%; KK 2 yang diberi larutan CMC 0,5% dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi larutan CMC 0,5% dan susu kedelai dengan fortifikan Fe fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah pencekokan hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah (P < 0,05) menunjukkan pengaruh nyata pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 27,90% terhadap KK 1 dan 17,49% terhadap KK 2.

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The effect of Fe fumarate fortificant addition in soy milk intake on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups, consist of normal control group (KK 1) which was administered with CMC 0.5% solution, treatment control group (KK 2) which was administered with CMC 0.5% solution and unfortified soy milk, and three treatment groups which were administered with soy milk added with fortificant Fe fumarate 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for consecutive 21 days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post-hoc LSD test (P < 0.05) showed significant effect of fortificant Fe fumarate addition in soy milk intake on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 1, which is 27.90% to KK 1 and 17.49% to KK 2."

"Penelitian untuk mengetahui pengaruh fortifikan NaFeEDTA dalam tepung tahu terhadap kadar hemoglobin darah tikus jantan telah dilakukan. Metode penelitian berupa Rancangan Acak Lengkap, terdiri atas 25 ekor tikus putih yang dibagi menjadi lima kelompok perlakuan yaitu KK1 yang diberi minuman, pakan standar dan larutan CMC 0,5 ; KK2 yang diberi minuman, pakan standar, larutan CMC 0,5 dan tepung tahu 0,45 g tanpa fortifikan; serta KP 1, KP 2, KP 3 yang diberi minuman, pakan standar, larutan CMC 0,5 dan tepung tahu 0,45 g dengan fortifikan NaFeEDTA dosis 2,7 mg Fe/kgBB; 5,4 mg Fe/kgBB; dan 10,8 mg Fe/kgBB selama 14 hari. Pengukuran kadar hemoglobin darah dilakukan pada hari ke-0 dan 14 menggunakan Hematology Analyzer. Hasil uji Saphiro-Wilk dan Levene menunjukkan data terdistribusi normal dan homogen. Uji ANAVA satu arah menunjukkan hasil pengaruh nyata pemberian fortifikan NaFeEDTA terhadap kadar hemoglobin antar kelompok perlakuan. Hasil uji LSD menunjukkan terdapat perbedaan kadar hemoglobin nyata antara KK1 dan KK 2 dengan KP 1, KP 2 dan KP 3; serta KP 2 dengan KP 1 dan KP 3. Peningkatan tertinggi kadar hemoglobin darah terdapat pada KP 2 yaitu sebesar 15,15 dalam kelompok; 11, 76 terhadap KK 1; dan 10,95 terhadap KK 2.

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Research to find out the effect of fortificant NaFeEDTA in tofu flour on blood hemoglobin levels in male rats has been done. Twenty five rats were divided into five groups, consist of normal control group KK1 which was given standard feed, drinks and solution of CMC 0.5 treatment control group KK 2 which was given standard feed, drinks, solution of CMC 0,5 , and 0,45 g tofu flour without fortificant and three treatment groups KP 1, KP 2, KP 3 which were given standard feed, drinks, solution of CMC 0,5 , and 0,45 g tofu flour with NaFeEDTA 2.7 mg Fe kgBB 5.4 mg Fe kgBB and 10.8 mg Fe kgBB for 14 days. Measurement of blood hemoglobin levels done on day 0 and 14 using Hematology Analyzer. One way ANAVA test shows significant effect of NaFeEDTA on hemoglobin levels in all treatment groups. LSD test shows the blood hemoglobin level was significantly different between KK1 and KK 2 with KP 1, KP 2, and KP 3 as well as KP 2 with KP 1 and KP 3. The highest increase in blood hemoglobin levels was detected on KP 2 which is 15.15 within the group 11, 76 against KK 1 and 10.14 against KK 2."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL). Sebanyak 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA berturut-turut dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah dan uji LSD (P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 3 di hari ke-21 yaitu 31,74% terhadap KK 1; dan 23,52% terhadap KK 2.

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The effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups. Normal control group (KK 1) which was administered with standard feeding and drinking only. Treatment control group (KK 2) which was administered with extra soymilk non fortificant, and three treatment groups which were administered with extra soymilk added with NaFeEDTA fortificant 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for 21 days consecutively. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post hoc LSD test (P < 0.05) showed significant effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 3 at t21 which is 31.74% to KK 1; and 23.52% to KK 2."

"ABSTRAKTelah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberianfortifikan NaFeEDTA dalam tepung tempe terhadap kadar zat besi plasma darah tikus(Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitianmenggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putihjantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu kelompok kontrol normal(KK1) yang hanya diberikan CMC 0,5%, kelompok kontrol perlakuan (KK2) yangdiberikan suspensi tepung tempe tanpa fortifikan dan kelompok perlakuan 1, 2, 3(KP1, KP2 dan KP3) yang diberikan suspensi tepung tempe dengan fortifikanNaFeEDTA dosis 1,35 mgFe/ kgBB, 2,7 mgFe/ kg BB, dan 5,4 mgFe/ kgBB.Pemberian bahan tersebut dilakukan secara oral selama 21 hari berturut- turut.Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan hari ke-21. KadarFe diukur dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji anava satuarah dan LSD (P < 0,05) terhadap sampel menunjukkan terdapatnya perbedaan nyatapemberian fortifikan NaFeEDTA dalam tepung tempe terhadap kadar zat besi selama21 hari. Peningkatan kadar zat besi tertinggi akhir penelitian (t21) terjadi pada KP 3,yaitu sebesar 27,40% terhadap KK1 dan 24,38% terhadap KK2.

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ABSTRACTThe study has been conducted to know the effect of fortificant NaFeEDTAadministration on tempeh flour to the plasma iron concentration of male rats (Rattus norvegicus L.). Twenty five male rats were divided to five groups consisting of normal control group (KK1) which was administered with CMC 0,5%; treatment control group (KK2) which was administered with tempeh flour without fortificant; and three treatment groups which were administered with tempeh flour and fortificant NaFeEDTA with different doses; 1,35 mgFe/KgBw (KP 1); 2,7 mgFe/KgBw (KP 2); and 5,4 mgFe/KgBw (KP 3). Treatments were carried out orally within 21""consecutive days. Blood is tested before treatment (t0) and after 21 days of treatment (t21). The plasma iron concentrations were measured by Atomic Absorption Spectrophotometer (AAS). Result was statistically tested with SPSS. One-way anova test (P < 0,05) and post hoc LSD test (P <0,005) showed that adding fortificant NaFeEDTA is giving a differences iron concentrations at blood levels of rats from the first day until last day of treatments. Increased iron levels are highest in the KP3 at day 21, which increased 27.40 % compared with KK 1 and 24,38% compared with KK 2."

"Telah dilakukan penelitian untuk mengetahui pengaruh pemberian simplisia Alphitobius sp. terhadap konsentrasi kolesterol total plasma darah tikus putih (Rattus norvegicus L.) jantan galur Sprague-Dawley. Dua puluh empat ekor tikus dibagi dalam 6 kelompok perlakuan yang terdiri atas kelompok kontrol normal yang hanya diberi pakan standar, kelompok kontrol perlakuan yang diberi diet tinggi lemak, dan 4 kelompok perlakuan yang diberi diet tinggi lemak dan bahan uji simplisia Alphitobius sp. dengan dosis 2,25 mg/kg bb, 4,5 mg/kg bb, 6,75 mg/kg bb dan 9 mg/kg bb. Pemberian bahan uji dan diet tinggi lemak dilakukan selama 14 hari berturut-turut. Hasil uji Anava satu arah (P < 0,05) menunjukkan terdapat pengaruh yang nyata terhadap konsentrasi kolesterol total akhir pada semua dosis perlakuan. Penurunan konsentrasi kolesterol terbesar dicapai oleh kelompok dosis 4,5 mg/kg bb yang juga merupakan dosis efektif dalam menurunkan konsentrasi kolesterol total yang mendekati nilai kelompok kontrol normal.

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A research was done in to determine the effect of simplicia Alphitobius sp. intake on plasma total cholesterol concentration in white male rat Sprague-Dawley (Rattus norvegicus L.). Twenty-four male rats were separated into six treatment groups consisting of normal control group that were given a standard diet, treatment control group fed a high fat diet , and four treatment groups that were given a high fat diet and simplicia Alphitobius sp. with a different dose (2,25 mg/kg bw, 4,5 mg/kg bw, 6,75 mg/kg bw and 9 mg/kg bw). Provision of test material was done every day for 14 consecutive days. The result of one-way Anava test (P < 0,05) indicates that there is a significant effect on total cholesterol concentration at all treatment doses, showed that provision of simplicia Alphitobius sp. lower total cholesterol potentially. The biggest decrease of total cholesterol concentrations was achieved by dose 4,5 mg/kg bw, which is also an effective dose in lowering total cholesterol concentrations approached normal value."