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"Telah dil p kukan uji teratogenisitas kiorpropamidapada mencit strain Biomedis, bertujuan untuk lebih dapatmeramalkan apakab kiorpropamida menyebabkan cacat bawaanpada manusia atau tidak. Dosis kiorpropamida yang diguriakanadalah 50 mg/kg BB, 200 mg/kg BB dan 400 mg/kg BB (lebihkurang setara dengan 5X, 20X dan 40X dosis lazim tertinggipada manusia), diberikan per oral melalul sondelambung. Dalam penelitian ml digunakan dua kelompok kon-.trol. Kelompok kontrol I tidak diberikan apa-apa kecualimakarian dan minuman ad libitum seperti halnya kelompok lainnya,sedangkan kelompok kontro]. II diberikan suspensiCNO (vehikel) 0,5%. Pemberian obat kepada kelompok keloladan suspensiCj kepada .kelompok' kontrol II dilakukan selama10 hari yaltu dari hari ke 6 sampal dengan 15 keharnilan.(selarna periode organogenesis). J1encit dimatikan pada harike 18 kehamilan dengan eter dan dilakukan histerektomi.- Pemeniksaan yang dilakukan : juml.ah fetus hidup dengan Cacatbawaan (eksternal dan internal termasuk cacat tulangrangka) yang dapat dilihat secara makroskopis dan jenisCacat bawaan yang terjadi, jumlah fetus hidup tanpa cacat,jumlah resorpsi dri fetus mati intrauterus. Juga dianalisakenaikari berat bp d.an mencit seisma kehamilan.Klorpropamlda dosis 50 mg/kg BB per hari tidk menunjukkanefek yang bermakna balk terhadap terjadinya Cacatbawaan (Fisher test), terhadap frekuensi resorpsi danfetus mati intrauterus (Chi-square test), maupun terhadapkenaikan berat badan induk mencit selama kehamilan (Testanalisa vanians satu arah dan student 't' test).Kiorpropamida dosis 200 mg/kg BB per . hari menunjukkanefek yang bermakna terhadap terjadinya cacat bawaan(Fisher test), tetapi tidak menunjukkan efek yang bermaknaterhada.p frekuensi resorpsl dam kematlan fetus intrauterus(Chi-square test) dan terhadap kenaikan berat badan indukmencit sela.ma kehmllan (Test analisa vanians satu arahdan student I t' test).Kiorpropamida dosis. 400 mg/kg BB per hari menunjukkanefek yang bermakna terhadap terjadlnya cacat bawaan(Fisher test), terhadap frekuensi resorpsi dan kematianfetus intrauterus (chi-square test) dan terhadap kenaikanberat badan induk mencit selama keharnhla.n (Test analisavanians satu srah dan student 't' test).Jenis cacat bawsan yang merupakan efek yang bermaknads.ri kiorpropamida adiah 'cleft palate' (Fisher test).

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The teratogenicity of chiorproparnide has been investigted

on Biomedis strain mice, in order to be able

to predict whether chiorpropamide causes congenital mall ormations

in man or not. The dosages of 50 mg/kg B.W., 200 mg/

kg B.W. and 400 mg/kg B.W. (equivalent to about 5, 20 9 and

40 times the higest usual dose in man) daily were used,,

They were given orally with a stomach tube. This investigation

had two control groups. The first one was given nothing

except diet given ad libitum and the second one was given

suspension of 0,5 % CMC. ChJorpropnmlde suspensions ind CMC

suspension were respectively given to treated groups of mice

and the second control group for 10 days i.e. on 6 to 15th

of gestation day (orgsnogenesis period). The animals were

sacrificed on 18th day of pregnancy by an over dose of ether.

The number of all living and intact, dead and resorbed, and

malformed fetuses (evaluated macroscopically), the kind of

malformations (external and internal malformations, including

skeletal defects) were observed and recorded. The weight

gain along the pregnancy were also analysed.

With dnily doses of 50 mg/kg B.W. , chlorpropimide

didn't show significant effects on the congenital anomalies

prevalence (Fisher test), the frequency of resorptions and

intrauterine deaths (Chi-square test) and the weight gain

along the pregnancy (Analysis of variance one way test and

Student 't' test).

With daily doses of 200 mg/kg B.W., chiorpropamide

showed significant effect on the congenital anomalies prevalence

(Fisher test), but it didn't show significant effect

on the frequency of resorptions and Intrauterine deaths

(Chi-square test) and the weight gain along the pregnancy

(Analysis of variance one way test and Student 't' test).

With daily doses of 400 mg/kg B.W., chiorpropamide

showed significant effects on the congenital anomalies prevalence

(Fisher test), the frequency of resorptions and intrauterine

deaths (Chi-square test) and the weight gain

along the pregnancy (Analysis of variance one way test and

Student I t' test).

The kind of congenital anomaly which was significant

effect of chiorpropamide was cleft palate (Fisher test)."

"Penelitian ini dilakukan untuk melihat pengaruh pemberian tartrazin dosis tunggal secara oral terhadap sistem tulang keras fetus mencit galur Swiss Derived. Pemberian tartrazin secara oral dilakukan pada hari kehamilan ke-7 terhadap 24 ekor induk dalam empat kelompok perlakuan, yaitu: 0,0000, 3,1875, 4,2500, dan 6,3750 gram tartrazin/kg berat badan dengan pelarut akuabidestilata. Induk dikorbankan pada hari kehamilan ke-18, kondisi intrauterin dicatat, lalu fetus diwarnai dengan Alizarin Red S. Pengamatan sis tem tulang keras fetus mencit dilakukan dengan membandingkan fetus perlakuan dan kontrol. Hasil Uji Jonckheere-Terpstra (50= 0,05) menunjukkan jumlah fetus yang diresorpsi dan persentase kematian pascaimplantasi cenderung meningkat sejalan dengan kenaikan dosis. Hasil Uji ANAVA menunjukkan tidak ada hubungan antara kegagalan implantasi dan berat rata-rata fetus tiap induk dengan peningkatan dosis.Hasil Uji Kruskal-Wallis («0 = 0,05) terhadap: (1) Perubahan waktu penulangan pada bagian cranium, c,v. cervicales dan thoracales,sternebrrae, dan costae tidak berbeda nyata. (2) Variasi sistem tulang keras pada bagian a.v. cervicales, sternebrae, dan costae tidak berbeda nyata; (3) Malformasi sistem tulang keras pada bagian c-v, cervicales dan thoracale, sternebrae, dan costae tidak berbeda nyata."

"Telah dilakukan uji teratogenitas Nat'iuñi. difeni1hi dantoin (Na-DPH) pada mencit strain Biomedis, bertujuan untuk memprediksi sensitivitas strain mi terhadap pemberian obat.Berat badan mencit antara 20-25 grain,berumur 2,5 bjlan.Dosis yang digunakan 50 mg/kg BB atau 100 mg/kg BB dan kontrol,dilarutkan dalam larutan 0.05% NaOH,diberikan secara intraperitoneal.Mencit yang hamil dibagi seca-ra random menjadi 12 subke1ompok.0bat diberikan selarna 3 hari dalam periode organogenesis pada hari ke 6 - 8; 9 - 11; 12.- 14 kehamilan serta dosistunggal pada hari ice 12kehamilan.Pemeniksaan fetus dilakukan setelah histerektomi pada hari ice 18 kehamilan.Dihitung jumlah fetus hidup tanpa cacat,yang. mengalami resorpsi dan mati intrauterus serta fetus cacat I yang dilihat secara rnakroskopik.Dianalisa juga berat badan mencit selama kehamilan.Dengan analisa statistic (Fisher test) menunjukkan pangaruh bermakna terhadap prevalensi cacat bawaan dibandingkan keiompok.kontroi Tidak terdapatpengaruh bermakna obat terhadap berat badan mencit selama kehamilan.

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The teratogenicity of Diphenyihydantoin Sodium (DPH -

Na) has been investigated on Biomed.is strain mjce,in order

to predict the sensitivity of 'this strain against DPH-Na.

The average body weight of the 2.5 months female mice were

20 to 25 gramThe dosages of 50 rag/kg B.W. or 100 mg/kg B.W

in 0.05% NaOH solution

were used, they were given intraperitoneally.

The pregnant mice were randomly devided into 12

sub groups based on the treatment schedule. Thedrug-were

given three days in their organogenesis period i.e on the

6th to 8th; 9th to llth;.i2tho 14th of gestation day and

a single dose on the 12th day.Fetus examination was carried

out after mice laparotomy on the 18th day of. pregnancy. The

numbers of all living and intact dead fo&tuses with resorption

a.nh foetuses with congenital anomalies were observed

and recorded.Thë weight gain along the pregnancy were also

analised.With statistical analyses (Fisher test) it was

shown that there were significant effect of PR-Na on the

congenital anomalies prevalence compared to the-control

group (p<0.05)-.There were significant effect (Fisher test),

of the administering days on the cleft palate prevalency

that were on the 9th - 11th;. 12th; 12th -.14th of gestation

day (p<0.05).There were also significant effect (Chi square

test)orte medicine on the resorption and intrauterine

deaths of all the groups (p<0..05).The effect of DPH-Na on.'

the weight gain along the pregnancy was not significant

(Anova two way test)."

"Tomato (Solanum lycopersycum L.) that the fruit mainly contained lycopene, beta carotene, vitamin C and vitamin E indECated that the fruit had antioxidant activity. These compound were known able to prevent and retention of free radECal forming whECh can cause aging and chronEC disease. This research, tomato with different concentration 0,5%, 1%, 2%, and 3% were formulated in cream. PhysECal stability test including the storage at three different temperatures including cool temperature (4°C), room temperature, and high temperature (40+2°C), mechanECal test, and cycling test. Measurement of antioxidant activity tomato cream that using DPPH method pursuant to value of DPPH retention (EC50). This research resulted that shown tomato cream 0,5% 1%, 2%, and 3% have physECal stability with storage at cool temperature (4oC), room temperature, and high temperature (40+2°C). Tomato cream 1%, 2%, and 3% reach minimum value of retention DPPH (EC50) but tomato cream 0,5% not reach minimum value of retention DPPH (EC50). Cream tomato 1% have the best physECal stability and cream tomato extract 3% have the best antioxidant activity."

"Phalerin is an active component of mahkota dewa (Phaleria macrocarpa (scheff.) Boerl) proven to have an anti inflamation effect. The labeling of phalerin with gamma emiting radionuclides was aimed to study is pharmacokinetic behavior and particularly to trace its metabolites. The labeling with I was caried our using iodogen as oxidator. Radiolabeled compound was characterized by high performance liquid choromatography (HPLC) using C-18 column eluted with methanol 70% and detected with UV detector (z=291 nm) and by thin layer chromatography (TLC) using silica gel strips eluted with chloroform - methanol (9:2), and lebeling efficiency was determined using the same TLC system. Purification of radiolabeled product was carried out using size exclusion chromatography (Sephadex G-25 column) eluted with 0.05 M phosphate buffer pH 7.4 Biodistributions of I-phalerin in various organs of normal and inflammation - induced mice were observed at 1,4 and 24 hours post-intravenous injection. radiochemical purity of I-phalerin was 90.2 krang lebih 2.8% and increased to 96.0 krang lebih0,4% after purification. Radioactivities in inflamed tissue at 1,4 and 24 hours post injection were respectively 1.6 times, 1,4 times and 1.3 times higher than that in normal tissue. The results showed a significant uptake of radiolabeled phalerin in inflamed."

"ABSTRAKTelah dilakukan penelitian untuk menguji potensi anti mutagenisitas ekstrak jahe (Zin giber officinale Roscoe) terhadap pembentukan mikronukleus pada sumsum tulang mencit yang diinduksi oleh mitomisin C. Mencit dicekok dengan ekstrak jahe dosis 0; 6,25; 12,5; 25; 50 dan 100 mg/kg berat badan (bb) selama 7 hari berturut-turut. Penghitungan mikronukleus per 1.000 eritrosit polikromatik dilakukan pada sediaan oles sumsum tulang yang telah diwarnai dengan pewarnaan May Gruenwald Giemsa. Hasil uji statistik menunjukkan bahwa ekstrak jahe memiliki aktivitas antimutagenik, yang dibuktikan dengan penurunan jumlah rata-rata mikronukleus pada eritrosit polikromatik setelah dicekok dengan dosis 6,25; 12,5; 25; 50 dan 100 mg/kg bb dibanding kontrol. Hal ini juga didukung oleh hasil uji Kruskal-Wallis yang menunjukkan adanya perbedaan yang nyata antara ke-6 kelompok perlakuan pada a = 0,05 dan uji perbandingan berganda yang menunjukkan jumlah mikronukleus rata-rata pada kelompok dosis 6,25; 12,5; 25; 50 dan 100 mg/kg bb berbeda nyata dengan kelompok kontrol pada a = 0,05. Tidak terdapat hubungan linier antara dosis pencekokan ekstrak jahe dengan penurunan jumlah mikronukleus."