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"Characterizations have been performed on single stage Transversely Excited Nitrogen Laser of Blumlein type. Optical cavity arrangement is varied prior to measurement which shows no significant increase in energy on both configurations of inserting a mirror only and a pair of mirror and a quartz parallel plate. Frequency repetitions respond reveals a small drop in energy at higher frequency i.e. 10 Hz by a factor of 20%. N2 flow rate consideration behaves on the similar way like pressure profile. At higher supply voltage maximum energy is shifted to higher N2 flow rate. A similar tendency occurs on pressure curve but with more pronounced maximum energy. Higher supply voltage would shift maximum energy to higher N2 pressure. Other operating conditions have been kept constant. Beam divergence measurement has given 1,87 mrad on vertical direction and 9,32 mrad on horizontal axis. However, beam cross section experiences a -10° tilt against horizontal reference. This may happen due to a slight twist on main electrodes. Measurements on different date have showed inconsistent results. Major cause is suspected on using different N2 cylinder, replacement on the gauge pressure, and crater creation on HV side spark gap electrode."

"ABSTRACTTwo laboratory-scale sequencing batch reactors (SBR) arranged in series were used to evaluate bioxidadon and nitrogen removal from a synthetic sewage consisting of acetic acid as the carbon source. Microprocessor based timer were used to control various phases in the SBR?s phases. This paper discusses the result of varying recycle ratio, returned mixed liquor, in bioxidation, denitrification and nitrification process in the modified SBR Generally, as the recycle ratio increased if was found to give a better the removal efficiency in terms of organic carbon and total nitrogen removal. The modified SBR, in general. shows promise as an effective treatment alternative to the conventional activated sludge process."

"ABSTRACTThe autofluorescence spectra of human tissues excited with 33'7,1-nm line of pulse nitrogen laser have been measured. The autofluorescence spectra of cancerous tissues consistently exhibit characteristic peaks near E530-nm and 690-nm. which do not appear in the spectra of the corresponding normal tissues. Spectroscopic investigation indicates that the porphyrin compounds localized and retained in cancerous tissues might be responsible for this characteristic autofluorescence. This preliminary result suggests the possibility of employing spectroscopy tool for complementary diagnosis of cancer along with the traditional methods of pathological analysis."

"Laporan praktikum ini bertujuan mendapatkan metode untuk  penetapan total nitrogen menggunakan reaktor COD sehingga proses analisis dapat dilakukan dengan lebih efektif dan efisien. Pekerjaan ini merupakan bagian dari kegiatan inovasi di perusahaan tempat penulis bekerja dan penulis bertanggungjawab sebagai koordinator laboratorium air limbah yang melakukan verifikasi terhadap hasil data Analisis.Modifikasi dilakukan pada tahapan preparasi sample yang seharusnya menggunakan autoklaf, dimodifikasi menggunakan reaktor COD. Karena adanya perubahan cara kerja pada metode standar, maka dilakukan validasi terhadap metode tersebut. Hasil validasi menunjukan nilai parameter linieritas yang didapatkan adalah  koefisien korelasi 0,9985. Untuk  akurasi 91,4%, Presisi RSD ≤ 0,67 CV horwitz 8,04 <  8,26, Untuk Method Detection Limit MDL didapat nilai 0,02 mg/L. Dari ketiga parameter tersebut bisa dijadikan kesimpulan bahwa metode dapat digunakan untuk pengujian rutin Total Nitrogen. Aspek etika Insinyur dan Profesionalisme laboratorium lingkungan merujuk pada PermenLHK no 23/2020, aspek Keselamatan dan Kesehatan Kerja juga dilakukan untuk memastikan kesesuaian kegiatan di laboratorium dengan program kerja K3L di perusahaan.

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This practical report aims to obtain a method for determining total nitrogen using a COD reactor so that the analysis process can be carried out more effectively and efficiently. This work is part of innovation activities at the company where the author works and the author bids as a wastewater laboratory coordinator who verifies the results of analysis data. Modifications were made at the sample preparation stage, which should have used an autoclave, modified using a COD reactor. Due to changes in the way the standard method works, validation of the method is carried out. The validation results show that the linearity parameter value obtained is a correlation coefficient of 0.9985. For an accuracy of 91.4%, Precision RSD ≤ 0.67 CV Horwitz 8.04 < 8.26. For the MDL Limit Detection Method, a value of 0.02 mg/L is obtained. From these third parameters it can be concluded that the method can be used for routine testing of Total Nitrogen. Aspects of Engineer Ethics and Professionalism in the Laboratory Environment Referring to Minister of Environment and Forestry Regulation No. 23/2020, Occupational Safety and Health aspects are also carried out to ensure the suitability of laboratory activities with the company's K3L work program."

"ABSTRAKPenelitian mengenai optimasi teknik hidrolisis selubung mucilage untuk meningkatkan konsentrasi dan kemurnian DNA Cyanobacteria filamen lurus yang ditumbuhkan pada medium dengan dan tanpa unsur nitrogen telah dilakukan. Penelitian bertujuan untuk mengetahui pengaruh homogenisasi dengan glass beads dan penambahan nitrogen pada medium terhadap penghilangan selubung mucilage strain Cyanobacteria filamen lurus. Strain Cyanobacteria yang digunakan adalah SO-24, SO-115, dan SO-163. Masing-masing strain uji ditumbuhkan di medium Blue-Green 11 BG-11 dengan dan tanpa unsur nitrogen, kemudian dilakukan homogenisasi dengan glass beads, hidrolisis selubung, dan ekstraksi DNA. Efektivitas optimasi teknik hidrolisis dilihat berdasarkan hasil pengamatan mikroskopik dengan dan tanpa pengecatan negatif, serta berdasarkan hasil pengukuran konsentrasi dan kemurnian DNA. Hasil penelitian menunjukkan proses homogenisasi tidak memberikan pengaruh terhadap penghilangan selubung mucilage, sedangkan penambahan nitrogen pada medium memengaruhi penghilangan selubung mucilage pada ketiga strain uji. Teknik hidrolisis efektif untuk menghilangkan selubung mucilage strain SO-24 pada medium dengan dan tanpa unsur nitrogen. Sementara itu, strain SO-115 dan SO-163 hanya efektif pada medium dengan unsur nitrogen. Ketiga strain uji menghasilkan konsentrasi DNA yang rendah yaitu berkisar dari 1,58 mdash;4,52 ng/ L dengan kemurnian DNA di bawah 1,8.

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ABSTRACTResearch on the optimization of hydrolysis techniques to improved DNA concentration and purity of filamentous Cyanobacteria which were grown in medium with and without nitrogen content has been conducted. The research aims to know the effect of homogenization with glass beads and addition of nitrogen in medium on mucilage sheath removal of filamentous Cyanobacteria. Strains of Cyanobacteria was used is SO 24, SO 115, and SO 163. Each strain was grown in Blue Green 11 BG 11 medium with and without nitrogen content, and then homogenized with glass beads, hydrolyzed process, and DNA was extracted. The effectivity of hydrolysis techniques was achieved based on microscopic observation with and without negative staining, and based on measuring result of DNA concentration and purity. The result showed that homogenization process doesn rsquo t affect mucilage sheath removal, however addition of nitrogen affects mucilage sheath removal. The hydrolysis techniques effective on mucilage sheath removal from strain SO 24 which was grown in medium with and without nitrogen content. Meanwhile, for strain SO 115 and SO 163 were effective only in medium with nitrogen content. The result of DNA concentration showed that all of strains showed low DNA concentration with value range from 1,58 mdash 4,52 ng L with DNA purity was below 1,8."

"ABSTRAKPenelitian mengenai optimasi teknik hidrolisis terhadap penghilangan selubung mucilage Cyanobacteria filamen bercabang pada strain SO-130, SO-133, dan SO-198 telah dilakukan. Optimasi tersebut ialah homogenisasi dan penambahan nitrogen dalam medium pertumbuhan. Penelitian bertujuan untuk menganalisis proses hidrolisis terhadap isolat uji yang ditumbuhkan pada medium dengan dan tanpa unsur nitrogen. Selain itu bertujuan untuk menganalisis pengaruh hidrolisis terhadap konsentrasi dan kemurnian DNA hasil isolasi. Ketiga strain yang digunakan dikultur menggunakan medium cair Blue Green BG 11 dengan dan tanpa nitrogen. Perlakuan hidrolisis selubung mucilage dilakukan sebanyak lima kali ulangan dengan modifikasi homogenisasi menggunakan glass beads BioSpect 3,2 mm sebanyak lima butir. Pengamatan mikroskopis filamen dilakukan pada setiap tahapan dengan dan tanpa pengecatan negatif. Hasil penelitian menunjukkan hilangnya selubung mucilage di ketiga strain uji yang ditumbuhkan pada medium dengan dan tanpa nitrogen setelah proses hidrolisis. Kemurnian DNA pada ketiga strain di setiap perlakuan berkisar dari 0,81 mdash;1,19, yang mengindikasikan belum didapatnya DNA murni. Konsentrasi DNA pada ketiga strain berkisar dari 1,63 mdash;6,47 ng/ L dan menghasilkan pola yang sama yaitu konsentrasi DNA mengalami penurunan dari kontrol ke perlak

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ABSTRACTch on the optimization of hydrolysis techniques of the removal of mucilage branching filamentous Cyanobacteria on SO 130, SO 133, and SO 198 strains has been done. The optimizations are homogenizing process and adding nitrogen in growth medium. The aim of the study was to analyze the process of hydrolysis based on the comparison of isolate culture medium in medium with and without nitrogen. The other aim was to analyze the effect of hydrolysis in purity and concentration of isolated DNA. The three strains used were cultured using a Blue Green BG 11 liquid medium with and without nitrogen. The hydrolysis treatment of the mucilage sheath was performed five replications with homogenization modification with five glass beads BioSpect 3,2 mm. The results showed that microscopic observations with and without negative staining yielded the same data for all three strains, that is the mucilage sheath was disappear after hydrolysis process in the three strains that cultured in medium with and without nitrogen. The DNA purity in all treatments results were at range 0,81 mdash 1,19 in the three strains, indicating the absence of pure DNA. The DNA concentration results were at range 1,63 mdash 6,47 ng L and have the same pattern in the three strains that is decreasing from control to treatment."

"Tingginya permintaan pengujian kualitas air permukaan di laboratorium komersil menyebabkan adanya antrian. Dengan adanya antrian, maka sampel yang diserahkan memiliki kemungkinan tidak langsung diuji sehingga sampel akan mengalami fase yang dinamakan fase waktu tunggu. Penelitian ini bertujuan untuk menganalisis perubahan nilai konsentrasi nitrogen selama rentang waktu tunggu. Selain itu, penelitian ini bertujuan menentukan laju perubahan selama proses nitrifikasi berdasarkan nilai perubahan konsentrasi yang terjadi. Kemudian penelitian ini bertujuan untuk memperkirakan nilai konsentrasi awal dari setiap parameter yang ada di dalam proses nitrifikasi. Adapun metode yang digunakan untuk mengetahui perubahan nilai konsentrasi nitrogen selama waktu tunggu adalah dengan melakukan pengujian laboratorium berdasarkan HACH. Sedangkan penentuan laju perubahan yang terjadi dilakukan dengan menggunakan metode Solver. Analisis perkiraan nilai konsentrasi awal dilakukan dengan pemodelan yang menggunakan solusi persamaan ODE Linear faktor pengintegrasian. Hasil pengujian laboratorium menunjukan adanya perubahan nilai konsentrasi amonia, nitrit, dan nitrat dari sampel yang didiamkan selama rentang waktu 14 hari dengan kondisi tanpa diawetkan dan dengan diawetkan. Rentang persentase perubahan nilai konsentrasi yang terjadi adalah sebesar 0,4% - 100% untuk amonia, 7% - 300% untuk nitrit, dan 18,2% - 960% untuk nitrat. Nilai laju perubahan amonia secara berurutan adalah 0,74/hari dan 0,4/hari untuk sampel tanpa pengawetan dan dengan pengawetan sedangkan nitrit adalah sebesar 0,05/hari dan 0,08/hari. Nilai error terkecil ada pada hari kedua untuk meperkirakan konsentrasi awal. Nilai konsentrasi amonia, nitrit, dan nitrat dengan kondisi tanpa pengawetan dan dengan pengawetan secara berurutan dengan menggunakan hari kedua sebagai waktu acuan adalah 17,29 dan 13,17 mg/L, 2,84 dan 15,26 mg/L, 0,93 dan 1,46 mg/L

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The high demand for surface water quality testing in commercial laboratories causes queues. With the existence of a queue, the samples submitted have the possibility of not being directly tested so that the sample will experience a phase called the holding time phase. This study aims to analyze changes in the value of nitrogen concentrations during the waiting period. In addition, this study tries to determine the level of change during the nitrification process based on the value of the changes in concentration that occur. Then this study aims to develop the concentration of the initial value of each parameter in the nitrification process. The method used to determine changes in the value of nitrogen concentration during the holding time is by conducting laboratory tests based on HACH. While the rate of change that occurs is done using the Solver method. Analysis of the estimated value of the initial concentration was carried out by modeling using the solution of the Linear integrating factor ODE equation. The results of laboratory tests showed that there was a change in the concentration values of ammonia, nitrite, and nitrate from the samples which were left for a period of 14 days with unpreserved and preserved conditions. The proportion of change in concentration value that occured is 0,4% - 100% for ammonia, 7% - 300% for nitrite, and 18,2% - 960% for nitrate. The value of the rate of change of ammonia respectively is 0,74/day and 0,4/day for samples without preservation and with preservation while for nitrite is 0,05/day and 0.08/day. Most of the smallest error value exist on the second day to estimate the initial concentration. The concentration values of ammonia, nitrite and nitrate under the condition of unpreserved and preserved respectively using the second day as a time reference were 17,29 and 13,17 mg/L, 2,84 and 15,26 mg/L, 0,93 and 1,46 mg/L."