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""Reflecting the enormous advances made in the field over the past ten years, this text synthesizes the latest developments in the ecology and evolution of animal parasites against a backdrop of parallel advances in parasite systematics, biodiversity and life cycles. This second edition has been thoroughly revised to meet the needs of a new generation of parasitology students. Balancing traditional approaches in parasitology with modern studies in parasite ecology and evolution, the authors present basic ecological principles as a unifying framework to help students understand the complex phenomenon of parasitism. Richly illustrated with over 250 figures, the text is accompanied by case study boxes designed to help students appreciate the complexity and diversity of parasites and the scientists who study them. This unique approach, presented clearly and with a minimum of jargon and mathematical detail, encourages students from diverse backgrounds to think generally and conceptually about parasites and parasitism"--"

"Banyak manfaat didapatkan dari mengkonsumsi sayuran. Namun sayuran dapat menjadi perantara penularan parasit usus (STH dan kista protozoa) dan meningkatkan angka kesakitan akibat infeksi ini. Berbagai penelitian membuktikan kontaminasi parasit usus diberbagai sayuran dengan jumlah beragam. Hal ini diperburuk dengan kebiasaan mengkonsumsi sayuran mentah (lalapan) di Indonesia. Maka dilakukanlah penelitian pada kubis karena sering dikonsumsi mentah.Penelitian ini ingin mengetahui perbedaan jumlah kontaminasi parasit usus yang ditemukan pada kubis pasar tradisional dan pasar swalayan serta ingin mengetahui efektifitas perendaman antara larutan garam-cuka dengan air. Penelitian ini adalah cross sectional dengan menggunakan 20 sampel kubis pasar tradisional dan 20 sampel kubis pasar swalayan yang dibeli secara acak di lima wilayah Jakarta.Hasil penelitian menunjukkan perbedaan bermakna (p=0,006) jumlah parasit yang ditemukan pada kubis pasar tradisional (2570 parasit) dan kubis pasar swalayan (1610 parasit). Ditemukan pada seluruh sampel (tradisional dan swalayan) terkontaminasi STH, 9 dari 20 sampel kubis pasar tradisional dan 7 dari 20 sampel kubis pasar swalayan terkontaminasi kista protozoa. Didapatkan pula perbedaan bermakna (p<0,05) jumlah parasit usus pada kubis yang direndam dengan larutan garam-cuka dibandingkan dengan air. Berdasarkan hasil penelitian ini jumlah parasit pada kubis pasar tradisional jauh lebih tinggi dan perendaman dengan larutan garam-cuka lebih baik dibandingkan dengan air.

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We can get a lot of benefit by consuming vegetables. But, vegetables also can be a medium to transmit parasites (STH and protozoa) and increase the morbidity because of infection. Some researches proved parasites contamination in any vegetables with vary of number. It is worsen by a habit to consume raw vegetables (lalap) in Indonesia. This research was done by using cabbage that often consume uncooked.This research wants to know the difference parasites quantity in cabbage from traditional and modern market and also the effectiveness between salt-acid solution and water as immersion medium. This cross-sectional research use 20 samples of cabbages from traditional market and 20 samples of cabbages from modern market in Jakarta.The result show a different parasites quantity (p value = 0,006) between cabbage from traditional market (2570 parasites) and modern market (1610 parasites). All of samples (traditional and modern market) are contaminated by STH, 9 of 20 cabbages from traditional market and 7 of 20 cabbages from modern market are contaminated by protozoa. And also there is a different parasites quantity (p value < 0,05) found in cabbage using salt-acid solution then water only. As conclusion number of parasites found in cabbages from traditional market is higher than cabbages from modern market and using salt-acid as immersion medium is better than water only."

"[ABSTRAKSayuran kemangi yang sering dikonsumsi secara mentah misalnya sebagai lalapan, dapat menjadi media transmisi infeksi parasit usus yaitu Soil Transmitted Helminths (STH) dan kista protozoa. Penelitian ini merupakan penelitian analitik observasional dengan metode potong lintang, menggunakan 40 sampel sayuran kemangi yang dibeli secara acak dari pasar tradisional dan swalayan di Jakarta. Dua puluh sampel dari pasar tradisional dan 20 sampel dari pasar swalayan kemudian direndam selama 24 jam dalam larutan garam cuka dan air sebagai kontrol. Perendaman ini dilakukan untuk memperoleh jumlah kontaminasi parasit usus. Data berupa jumlah telur STH atau kista protozoa kemudian diproses dengan SPSS versi 20 dan dianalisis dengan uji t tidak berpasangan atau uji Mann Whitney. Hasil penelitian menunjukan 14 sampel terkontaminasi STH, 7 sampel dari pasar tradisional dan 7 sampel dari pasar swalayan, dan seluruh sampel (100%) terkontaminasi kista protozoa. Jumlah parasit usus yang ditemukan sebesar 1780 pada pasar tradisional dan 1550 pada pasar pasar swalayan. Hasil penelitian ini menunjukkan bahwa tidak terdapat perbedaan bermakna (p > 0,05) antarajumlah kontaminasi parasit usus yang ditemukan pada pasar tradisional dan swalayan Jakarta, dan diperoleh perbedaan bermakna (p<0,05) antara jumlah parasit usus yang ditemukan pada media perendaman larutan garam cuka dan air. Jenis pasar tidak mempengaruhi kontaminasi parasit usus pada sayuran kemangi dan penggunaan larutan garam cuka sebagai media perendaman berpengaruh terhadap jumlah parasit usus yang ditemukan.

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ABSTRACTBasil is often consumed uncooked, instance as lalapan, but it can be a medium of transmission of the intestinal parasites infection, Soil Transmitted Helminthes (STH) and protozoan cysts. This research used an observational analytic crosssectional method, which used 40 samples of basil purchased randomly from the traditional and selfservice markets in Jakarta. Twenty samples from traditional markets and 20 samples from selservice markets were soaked in acetous salt solution and water as a control study to obtain the number of STH eggs or protozoan cysts. Data were processed by SPSS 20 version then analyzed by t test or Mann Whitney. Result of research showed 14 samples were contaminated by STH, 7 from traditional markets and 7 from selfservice markets, and all samples (100%) were contaminated by protozoan cysts. The number of parasites is 1780 from traditional markets and 1550 from selfservice markets. Results of this research showed, there was no significant difference (p>0.05) between the prevalence of intestinal parasites in traditional and selfservice markets in Jakarta, and there was significant difference (p<0.05) between the prevalence of intestinal parasites by sedimentation method in acetous salt solution and water. Type of market does not affect the prevalence of intestinal parasites in basil, and acetous salt solution as soaking media in sedimentation method, affects the prevalence of parasites.;Basil is often consumed uncooked, instance as lalapan, but it can be a medium of transmission of the intestinal parasites infection, Soil Transmitted Helminthes (STH) and protozoan cysts. This research used an observational analytic crosssectional method, which used 40 samples of basil purchased randomly from the traditional and selfservice markets in Jakarta. Twenty samples from traditional markets and 20 samples from selservice markets were soaked in acetous salt solution and water as a control study to obtain the number of STH eggs or protozoan cysts. Data were processed by SPSS 20 version then analyzed by t test or Mann Whitney. Result of research showed 14 samples were contaminated by STH, 7 from traditional markets and 7 from selfservice markets, and all samples (100%) were contaminated by protozoan cysts. The number of parasites is 1780 from traditional markets and 1550 from selfservice markets. Results of this research showed, there was no significant difference (p>0.05) between the prevalence of intestinal parasites in traditional and selfservice markets in Jakarta, and there was significant difference (p<0.05) between the prevalence of intestinal parasites by sedimentation method in acetous salt solution and water. Type of market does not affect the prevalence of intestinal parasites in basil, and acetous salt solution as soaking media in sedimentation method, affects the prevalence of parasites.;Basil is often consumed uncooked, instance as lalapan, but it can be a medium of transmission of the intestinal parasites infection, Soil Transmitted Helminthes (STH) and protozoan cysts. This research used an observational analytic crosssectional method, which used 40 samples of basil purchased randomly from the traditional and selfservice markets in Jakarta. Twenty samples from traditionalmarkets and 20 samples from selservice markets were soaked in acetous salt solution and water as a control study to obtain the number of STH eggs or protozoan cysts. Data were processed by SPSS 20 version then analyzed by t test or Mann Whitney. Result of research showed 14 samples were contaminated by STH, 7 from traditional markets and 7 from selfservice markets, and all samples (100%) were contaminated by protozoan cysts. The number of parasites is 1780from traditional markets and 1550 from selfservice markets. Results of this research showed, there was no significant difference (p>0.05) between the prevalence of intestinal parasites in traditional and selfservice markets in Jakarta, and there was significant difference (p<0.05) between the prevalence of intestinal parasites by sedimentation method in acetous salt solution and water. Type ofmarket does not affect the prevalence of intestinal parasites in basil, and acetous salt solution as soaking media in sedimentation method, affects the prevalence of parasites.;Basil is often consumed uncooked, instance as lalapan, but it can be a medium of transmission of the intestinal parasites infection, Soil Transmitted Helminthes (STH) and protozoan cysts. This research used an observational analytic crosssectional method, which used 40 samples of basil purchased randomly from the traditional and selfservice markets in Jakarta. Twenty samples from traditionalmarkets and 20 samples from selservice markets were soaked in acetous salt solution and water as a control study to obtain the number of STH eggs or protozoan cysts. Data were processed by SPSS 20 version then analyzed by t test or Mann Whitney. Result of research showed 14 samples were contaminated by STH, 7 from traditional markets and 7 from selfservice markets, and all samples (100%) were contaminated by protozoan cysts. The number of parasites is 1780from traditional markets and 1550 from selfservice markets. Results of this research showed, there was no significant difference (p>0.05) between the prevalence of intestinal parasites in traditional and selfservice markets in Jakarta, and there was significant difference (p<0.05) between the prevalence of intestinal parasites by sedimentation method in acetous salt solution and water. Type ofmarket does not affect the prevalence of intestinal parasites in basil, and acetous salt solution as soaking media in sedimentation method, affects the prevalence of parasites., Basil is often consumed uncooked, instance as lalapan, but it can be a medium of transmission of the intestinal parasites infection, Soil Transmitted Helminthes (STH) and protozoan cysts. This research used an observational analytic crosssectional method, which used 40 samples of basil purchased randomly from the traditional and selfservice markets in Jakarta. Twenty samples from traditionalmarkets and 20 samples from selservice markets were soaked in acetous salt solution and water as a control study to obtain the number of STH eggs or protozoan cysts. Data were processed by SPSS 20 version then analyzed by t test or Mann Whitney. Result of research showed 14 samples were contaminated by STH, 7 from traditional markets and 7 from selfservice markets, and all samples (100%) were contaminated by protozoan cysts. The number of parasites is 1780from traditional markets and 1550 from selfservice markets. Results of this research showed, there was no significant difference (p>0.05) between the prevalence of intestinal parasites in traditional and selfservice markets in Jakarta, and there was significant difference (p<0.05) between the prevalence of intestinal parasites by sedimentation method in acetous salt solution and water. Type ofmarket does not affect the prevalence of intestinal parasites in basil, and acetous salt solution as soaking media in sedimentation method, affects the prevalence of parasites.]"

"ASBTRAKRuang Lingkup dan Cara Penelitian: Di Indonesia kasus infeksi oleh cacing Echinostoma spp. belum banyak dilaporkan, tetapi di beberapa tempat tertentu ditemukan secara endemi. Infeksi pada manusia terjadi secara kebetulan, yaitu bila manusia makan keong air yang mengandung metaserkaria dalam keadaan mentah atau setengah matang.Tujuan umum penelitian ini adalah ingin mengetahui keadaan infeksi cacing Echinostoma spp. pada keong Bellamya javanica (Vivi para javanica) yang merupakan sumber infeksi bagi manusia di Indonesia. Sejumlah 2500 keong telah dikumpulkan, dan secara acak dipilih 500 ekor untuk dilakukan pembedahan dan pemeriksaan metaserkaria.Metaserkaria yang dikumpulkan diinfeksikan terhadap mencit putih. Telah diinfeksi 30 ekor mencit- putih, masing-masing dengan 150 ekor metaser karia. Untuk keperluan identifikasi, cacing dewasa yang tumbuh dalam usus mencit dikumpulkan, kemudian dipulas dengan teknik pulasan 'trichrome' yang dimodifikasi.Hasil dan Kesimpulan: Pada penelitian ini didapatkan angka infeksi metaserkaria Echinostoma, spp. setinggoi 100 % pada keong B. javanica. Rata-rata tiap keong mengandung 802 ekor metaserkaria. Infektivitas metaserkaria pada mencit cukup Tinggi, yaitu dari 30 ekor mencit terdapat 27 ekor {90%) positif, sedangkan jumlah produksi seluruhnya 133 ekor cacing. Jadi tiap mencit rata-rata mengandung 9 ekor cacing.Hasil identifikasi spesies diperoleh 75 ekor (56,4%) E. recurvatum, 24 ekor (18,0%) E. ilocanum, dan 10 ekor (7,6%) E. revolutum; lainnya 24 ekor (18,0%) tidak dapat diidentifikasi. Dengan demikian dapat dikimpulkan bahwa keong B. javanica merupakan hospes perantara II cacing Echinostoma spp. yang sesuai dan berperan sebagai sumber infeksi potensial bagi manusia.

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ABSTRACT

Scope and Method of Study: Cases of echinostomiasis are rarely reported in Indonesia, but in some places endemic foci have been found and are considered as of . public health importance. Human infections occurred accidentally, and man got the infection by way of consuming raw or half cooked snails which contained metacercariae. The general objective of this study is to know whether Echinostoma spp. larvae found in B. javanica (Vivipara javanica) snails are the potential source of infection for man in Indonesia. In this study 2500 snails were collected, and 500 snails were randomly selected for dissecting and searching for metacercariae. Experimental infection of 30 white mice were then carried out with 150 metacercariae for each mouse. For species identification, adult worms were stained by a modified trachoma staining technique.

Findings and Conclusions: The infection rate of Echinostoma in B. javanica was found to be 100 %, with a mean number of 802 metacercariae for each snail. The infectivity of metacercariae for white mice is quite high: of 30 mice infected, 27 (90%) were positive. A total of 133 adult worms were found; the worms found in each mouse varied from 1 - 27, with a mean of 5 worms per mouse. Identification results: 75 (56.5%) were identified as E. recurvatum, 24 (18.2%) E. ilocanum, 10 (7.6%) E. revolutum, and 24 (18.2%) could not be identified. Thus, based on this evidence, the snail B. javanica could be considered as a potential host for Echinostoma spp."