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Ditemukan 12020 dokumen yang sesuai dengan query
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Coppola, Gary M.
New York: John Wiley & Sons, 1987
547.2 COP a
Buku Teks SO  Universitas Indonesia Library
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London: Academic Press, 1991
581.19 MET
Buku Teks  Universitas Indonesia Library
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London: Academic Press, 1991
581.19 MET
Buku Teks  Universitas Indonesia Library
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Dupont, A.
"ABSTRACT
The statement contained in the motto cited above, made as early as the year 1906, still has lost nothing of its value for the present time. Although innumerable investigations have been carried out on proteins, and much insight was gained from this, a lot of problems connected with the proteins, remain to be solved. As is well known, proteins are found in nature in the animal and vegetable kingdoms. The differences between these two groups of proteins are situated in the number and the amount of the various acids present in these proteins.
Up to comparatively recent years, however, the knowledge of the requirement for the amino acids in the diet was limited to in-formation obtained with the young rat. This animal was able to grow when receiving only nine amino acids: namely histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine; and grew significantly better when arginine was also provided. These ten amino acids were called "essential" for the growing rat, the remaining amino acids were called "non essential". However, there was no implication that the dietetically non-essential amino acids are of little or no value. In spite of the cautions issued, there has been a general tendency to assume, that the above classification has a very broad application. The investigations made by Rose et all. (1,2,3,4,5), have revealed that for adult man only eight amino acids are essential, namely, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine. Moreover in these investigations, it was demonstrated, that only foods with sufficient caloric and nitrogen supply and adequate amounts of these eight amino acids simultaneously are able to main﷓ thin a nitrogen balance. This fact is of far reaching importance, because, from this it follows, that the nutritive value of a meal, with respect to the proteins, is determined by the total amount of essential amino acids - in the proper proportions - present in the constituents of the meal. In Indonesia, rice is the most important constituent of a meal. However rice contains such a small amount of some essential amino acids, that even larger use is not sufficient to meet the requirements of man. Therefore, other foods, which complete this shortage, must be used in combination with rice.
In general, in Indonesia, foods containing animal protein are relatively scarce; therefore, it is necessary to replace these with foods containing vegetable protein. An important source of plant protein is found in the soybean. As a general rule, the soybean yields fair crops, without asking too much care. Botanically, the soybean has been referred to as Glycine hispida (Moench) Max. Recent studies indicate that the correct botanical name should be Glycine max (L.) Merril according to international botanical rules (6).
The early history of the soybean, like that of most important food crops, is lost in obscurity. In the Far East story tellers for centuries have related, tales of the remarkable history of the soybean. Ancient Chinese literature reveals that the soybean was extensively cultivated and highly valued as a food. There it is said to be one of the grains planted by one of the gods of agriculture, named Hou Tsi. The first written record of the plant is contained in the books Pen Tsao Kong Mu, a materia medica written by Emperor Sheng Nung in 2838. B.C.
In many of the early writings advice of agricultural experts is given on soil preferences, proper time of planting, methods and rates of planting, the best varieties to plant under different conditions and for different uses, time to harvest, methods of storage, and utilization of the many varieties for different purposes. Some of this expert advice goes as far back as 2207 B.C. The soybean was included in the second class of drugs, and was regarded as having many medicinal virtues; it was regarded as a specific remedy for the propel functioning of the heart, and other organs.
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1954
D114
UI - Disertasi Membership  Universitas Indonesia Library
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Arif Kurniawan
"Latar Belakang: Sel kanker membutuhkan asam amino untuk metabolismenya telah ditemukan pada beberapa studi. Metabolisme ini meyebabkan profil asam amino mengalami perubahan pada kanker payudara. Profil asam amino pada kanker payudara ini dapat menentukan prognosis penyakit pada kanker payudara.
Tujuan: Mengetahui hubungan profil asam amino dengan faktor risiko kanker payudara di RS Ciptomangunkusumo, Indonesia.
Metode: Penelitian ini merupakan penelitian cross-sectional pada 19 subjek kanker payudara dan 19 subjek kontrol yang memenuhi kriteria penelitian di RSCM, Jakarta, Indonesia. Asam amino dianalisis menggunakan teknik liquid chromatography. Data tersebut dianalisis menggunakan uji independent sample t-test dan uji Mann-Whitney U.
Hasil:
Perbedaan profil asam amino pada kanker payudara yang signifikan dibandingkan dengan kontrol ditemukan pada peningkatan asam amino sistin dan penurunan asam amino valin, lisin, histidin, alanin, ornitin, tirosin, glutamin, fenilalanin, dan asam amino prolin. Terdapat signifikansi antara asam amino dengan faktor risiko usia, jumlah paritas, riwayat pemberian ASI, usia menarke, dan kebiasaan berolahraga.

Background: Cancer cell needs amino acids for its metabolism has been found in recent studies. Amino acids profile changes due to cancer cells’ metabolism. This amino acids profile in breast cancer could determine the prognosis of disease in breast cancer.
Objective: To aimed relationship between amino acids profile in breast cancer with its risk factor in Ciptomangunkusumo Hospital, Indonesia.
Method: A cross-sectional study in 19 breast cancer subjects and 19 control subjects that included into the criteria was conducted in this research. Amino acids was examined with liquid chromatography technique. The data was analyzed with independent sample t-test and Mann-Whitney U test.
Result: There are significant differences of amino acids profile between breast cancer subjects and control subjects, essentially for increased cystine profile and decreased valine, lysine, histidine, alanine, ornithine, tyrosine, glutamine, phenylalanine, and proline. There are also significance between amino acids profile in breast cancer with risk factors, including age, parity, breastfeeding history, menarche age, and workout habit.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
T-pdf
UI - Tesis Membership  Universitas Indonesia Library
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Speakman, J.C.
New York: McGraw-Hill, 1966
541.22 SPE m
Buku Teks SO  Universitas Indonesia Library
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Speakman, J.C.
New York: McGraw-Hill, 1966
541.22 SPE m
Buku Teks SO  Universitas Indonesia Library
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Irsan Hasan
"Background: The determination of branched chain amino acids (BCAA) to tyrosine ratio (BTR) was available in making differentiation of chronic hepatitis from liver cirrhosis, because there was a strong association between BTR and staging (fibrosis) scores. Branched chain ammo acids to tvrosine ratio have a correlation with Fischer ratio and the examination is easier because it can be done by enzymatic assay.
Materials and Methods: To evaluate the correlation between BTR and Child-Pugh score, we examined the amino gram of 52 liver cirrhosis patients consisted of 26 Child-Pugh A, 19 Child-Pugh B, and 7 Child-Pugh C. The examination of amino gram was done by High Pressure Liquid Chromatograph (HPLC) analyzer. Branched chain amino acids to tyrosine ratio were compared to Child-Pugh score, albumin, ammonia level, number connection test to Fischer ratio.
Results: Significant differences in BTR among Child-Pugh A, B, C were observed (Child-Pugh A 7.75 ± 1.2; Child Pugh B 6.0 ± 1.23 and Child Pugh C 4.38 + 3.14 (p = 0.000)). Branched chain amino acids to tyrosine ratio had a weak correlation with albumin fr - 0.292; p = 0.036), ammonia level (r = 0.376; p = 0.006) and strong correlation with Fischer ratio (r = 0.818; p = 0.000). There was no significant correlation between BTR and number connection test.
Conclusion: These results showed that the determination of the molar ratio of branched chain amino acids to tyrosine well reflected the severity of liver cirrhosis and it can be used as a substitute of Fischer ratio."
2005
IJGH-6-1-April2005-1
Artikel Jurnal  Universitas Indonesia Library
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Alberto Christopher
"Apoptin merupakan protein dari virus anemia ayam yang dapat menginduksi apoptosis sel kanker. Penggunaannya sebagai senyawa antikanker dapat mengatasi kelemahan metode kemoterapi. Deteksi massa molekular apoptin dilakukan dengan SDS-PAGE memiliki keberagaman hasil dan asam-asam amino rekombinan dalam apoptin disinyalir menyadi penyebabnya. Karakterisasi asam-asam amino rekombinan dalam apoptin untuk membuktikan hipotesis tersebut dilakukan dengan dua variasi rekombinan plasmid, yakni modifikasi 12 histidin dan modifikasi 12 histidin-8 arginin yang ditransformasikan pada Escherichia coli DH5α. Kedua variasi modifikasi plasmid ini mendapatkan perlakuan yang sama. Transformasi plasmid dalam penelitian ini menggunakan metode heat shock dengan suhu 42oC dan bantuan CaCl2 dalam pembentukan sel Escherichia coli DH5α kompeten. Penentuan konsentrasi apoptin dilakukan dengan metode Lowry dan BSA sebagai protein standarnya. Deteksi massa molekular apoptin oleh metode SDS-PAGE dilakukan dengan konsentrasi gel sebesar 15% dan mendeteksi adanya pita protein di bawah 30 KDa. Uji karakterisasi asam amino yang dilakukan dengan Liquid Chromatography Mass Spectroscopy menunjukkan bahwa adanya konsentrasi asam amino berlebih dalam apoptin sehingga meningkatkan deteksi massa molekularnya oleh SDS-PAGE.

Apoptin is a protein from chicken anemia virus which could induce tumor cell apoptotic. The use of apoptin as anticancer could overwhelm chemotheraphy weaknesses. Apoptin molecular weight detection conducted by SDS-PAGE had various results while the recombinant amino acids are the suspects. Recombinant amino acids characterization of apoptin in order to prove the hypothesis was conducted by two variants of recombinant plasmid, which were 12 histidine modification dan 12 histidine-8 arginine modification, transformed into Escherichia coli DH5α. These two variations were given the same treatment. Heat shock method was used in plasmid transformation at 42oC and CaCl2 treatment was used in order to create Escherichia coli DH5α competent cells. Apoptin concentration determination was conducted by Lowry method and BSA was used as protein standard. Molecular weight detection of apoptin by SDS-PAGE was conducted using 15% gel concentration and there was protein band detected below 30 KDa. Amino acids characterization test conducted indicate that there are excess amino acids concentration in apoptin as the cause of increasing molecular weight detection by SDS-PAGE.
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Depok: Fakultas Teknik Universitas Indonesia, 2014
S55247
UI - Skripsi Membership  Universitas Indonesia Library
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