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"ABSTRAKPendahuluan: Fusi spinal posterolateral adalah prosedur sering dilakukan dalam tindakan arthrodesis spinal yang menggunakan autograft dari krista iliaka. Penggunaan autograft memiliki keterbatasan dan komplikasi. Tingkatpseudoarthrosis berkisar antara 5-35%. Penggunaan platelet rich plasma (PRP) sebagai faktor osteoinduktif memiliki dasar ilmiah. Platelet dengan cepatmenempel pada permukaan tandur tulang dan mengalami degranulasi, kemudian melepaskan faktor-faktor pertumbuhan yang memicu penyembuhan tulang dan inkorporasi tandur. Studi ini bertujuan untuk mengetahui efek pemberian PRP terhadap tingkat fusi pada tindakan fusi spinal pada kelinci putih New Zealand Metode: Fusi posterolateral dilakukan pada 16 ekor kelinci putih New Zealand yang terbagi dalam 2 kelompok, yaitu kelompok fusi dengan autograft dan fusi dengan autograft + PRP. PRP diambil dari vena perifer lalu disentrifugasi, kemudian dilanjutkan dengan tindakan fusi posterolateral, pengambilan tandur krista iliaka, dan aplikasi PRP. Observasi dilakukan selama 8 minggu. Evaluasi dinilai secara radiologis dengan modifikasi skor Bridwell dan histologis dengan skor Huo/Friedlaender.Hasil: Secara radiologis, pada kelompok perlakuan fusi spinal dengan autograftdidapatkan 5 sampel definite fusion, 1 sampel probable fusion, dan 2 sampelnonunion. Sementara itu, pada kelompok perlakuan fusi spinal dengan autograft + PRP terdapat 5 sampel definite fusion, 3 sampel probable fusion, dan tidak ada nonunion. Namun, tidak terdapat perbedaan radiologis yang bermakna. Kelompok fusi spinal dengan autograft + PRP memiliki rerata dan median skor histologis yang lebih tinggi dibandingkan kelompok fusi spinal dengan autograft saja secara bermakna (8 vs 6,5).Simpulan: Pemberian PRP bersama dengan autograft pada fusi spinalposterolateral memiliki pengaruh terhadap tingkat fusi pada fusi spinal padakelinci putih New Zealand. Pemberian PRP dapat dipertimbangkan dan ditelitilebih lanjut sebagai faktor osteoinduktif alternatif dalam fusi spinal posterolateral

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ABSTRACTIntroduction: Posterolateral spinal fusion is a common procedure in spinal arthrodesis using autograft from iliac crest. Autografts utilization possess limitations and complication with pseudoarthrosis ranging between 5-35%. Platelet rich plasma (PRP) usage as an osteoinductive factors is based on scientific reasons. Platelets can quickly adhere to the surface of the bone graft and degranulate, releasing growth factors afterwards and inducing bone healing and graft incorporation. This study aim to establish the effect of PRP administration on fusion rate for spinal fusion on New Zealand white rabbit.Method: Posterolateral fusions were done on 16 white New Zealand rabbits divided into two groups: fusion with autograft and fusion with autograft + PRP. PRP was taken from peripheral vein and centrifuged. Posterolateral fusion and graft harvesting from iliac crest were followed by PRP application. Observation was done for 8 weeks. Evaluation was done radiologically with modified Bridwell score and histologically with Huo/Friedlaender score.Results: Radiologically, for spinal fusion with autograft group, the result were 5 definite fusion, 1 probable fusion, and 2 nonunion fusion. For spinal fusion with autograft + PRP, there was 5 definite fusion, 3 probable fusion, and no nonunion. There was no significant difference radiologically. Spinal fusion with autograft + PRP had higher mean and median for histological score compared to spinal fusion with autograft (8 vs 6.5).Conclusion: PRP administration with autograft for posterolateral spinal fusion affected fusion rate for spinal fusion on New Zealand white rabbit. PRP administration can be considered and studied further as an alternative for osteoinductive factors for posterolateral spinal fusion"

"In this paper ,the growth of GaN:Mn thin films by plasma-assisted metalorganic chemical vapor deposition (PAMOCVD) method is reported ..."

"This study was designed to optimalize the use of opididymal or ejaculate sperm and plasma for in vitro fertilization,that sperm agglutination was found at preparation. The rate of sperm agglutination was calculated the head-to-head sperm agglutination that were incubated in KR-HEPES medium in 38.5 oC with 5% CO2 at 1,3,5 and 7 hours culture in vitro....."

"Di masa kini, keamanan bahan pangan telah menjadi salah satu fokus utama bagi seluruh komponen sektor pangan, karena meningkatnya kesadaran masyarakat akan makanan yang aman, lezat, dan sehat. Patogen asing dan mikroorganisme pengurai merupakan mikroorganisme yang paling bertanggung jawab atas wabah penyakit yang ditularkan melalui makanan. Terdapat beberapa teknik termal konvensional yang dapat digunakan untuk membasmi mikroorganisme ini, seperti pasteurisasi, sterilisasi, pemanasan ohmik, autoklaf, dll. Namun, teknik-teknik ini semakin kurang diminati di pasaran, karena penerapan panas yang sangat tinggi berdampak buruk pada atribut kualitas fisiko-kimia, nutrisi, dan sensorik dari makanan yang diolah. Oleh sebab itu, berbagai teknologi alternatif non-termal telah dikembangkan dan dipelajari untuk memperoleh bahan pangan yang tetap aman dengan minimnya penggunaan bahan pengawet dan bebas bahan kimia, namun tetap mempertahankan sifat alaminya seperti kualitas sensorik dan gizi dalam waktu yang lama. Sebagai solusi alternatif, salah satunya dapat digunakan Plasma Activated Water (PAW). Metode generasi PAW yang optimal dan sering digunakan adalah Dielectric Barrier Discharge (DBD), karena efisiensinya dalam menghasilkan plasma dan kemudahan pengaplikasiannya dalam skala besar. Pada penelitian ini, akan dicipatakan Plasma Activated Water (PAW) menggunakan metode produksi Dielectric Barrier Discharge (DBD) dengan alat Ozone Generator. Agar sesuai dengan perkembangan zaman, maka peralatan Ozone Generator ini dicoba dilengkapi dengan sistem nyala-mati otomatis, agar campur tangan manusia dapat diminimalisir dalam pengendalian sistem sebisa mungkin. Dari percobaan, diukur parameter pH, konduktivitas, dan tingkatan ORP, dengan memerhatikan perkembangan parameter tersebut berdasarkan variasi percobaan volume air 100 mL, 200 mL, dan 300 mL, dan waktu treatment 1 menit, 3 menit, dan 5 menit. Hasil perkembangan parameter yang diukur diperoleh sudah sesuai dengan karakteristik terbentuknya PAW, yang ditemukan pada berbagai jurnal ilmiah yang meneliti karakteristik fisikokimiawi pada PAW. Sehingga, dapat dikatakan sistem Plasma Generator sederhana ini berhasil memproduksi PAW, dimana dengan parameter-parameter yang berhasil diukur, memiliki potensi untuk menjaga berbagai kualitas fisikokimia, sensorik, dan nutrisi pada bahan pangan lebih tahan lama.

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Nowadays, food safety has become one of the main focuses for all components of the food sector, due to increasing public awareness of safe, delicious and healthy food. Foreign pathogens and decomposing microorganisms are the microorganisms most responsible for food-borne disease outbreaks. There are several conventional thermal techniques that can be used to eradicate these microorganisms, such as pasteurization, sterilization, ohmic heating, autoclaving, etc. However, these techniques are becoming less popular on the market, as the application of very high heat has an adverse impact on the physico-chemical, nutritional and sensory quality attributes of the processed food. For this reason, various alternative non-thermal technologies have been developed and studied to obtain food ingredients that remain safe with minimal use of preservatives and are free of chemicals, but still maintain their natural properties such as sensory and nutritional qualities for a long time. As an alternative solution, one of them can be used Plasma Activated Water (PAW). The optimal and frequently used PAW generation method is Dielectric Barrier Discharge (DBD), because of its efficiency in producing plasma and ease of application on a large scale. In this research, Plasma Activated Water (PAW) will be created using the Dielectric Barrier Discharge (DBD) production method with an Ozone Generator. In order to be in line with current developments, the Ozone Generator equipment has been tried to be equipped with an automatic on-off system, so that human intervention can be minimized in controlling the system as much as possible. From the experiment, the parameters pH, conductivity, and ORP level were measured, by paying attention to the development of these parameters based on experimental variations in water volumes of 100 mL, 200 mL, and 300 mL, and treatment times of 1 minute, 3 minutes, and 5 minutes. The results of the development of the measured parameters were found to be in accordance with the characteristics of PAW formation, which were found in various scientific journals that examined the physicochemical characteristics of PAW. So, it can be said that this simple Plasma Generator system has succeeded in producing PAW, which, with the parameters that have been successfully measured, has the potential to maintain various physicochemical, sensory and nutritional qualities in foodstuffs that last longer."

"A study was performed on a laser-induced shock wave plasma generated on high concentration Au-Ag-Cu alloys by a Q-switched Nd-YAG laser of 4.8 mJ under reduced air pressure of 2 Torr. It was found that the total emission intensity of the secondary plasma is proportional to the intensity of the primary plasma, Assuming linear proportionality between the intensity of the primary plasma and the number of atoms vaporized from the target, it is proposed that the quantitative analysis can be applied to the intensities of the analytical emission Iines normalized by the total intensity of the primary plasma. This experimental result demonstrated for each metal element shows an excellent linear relationship between the normalized emission line intensity and the content of corresponding element after primary plasma normalization."