Hasil Pencarian  ::  Simpan CSV :: Kembali

"DNA forensik adalah teknik identifikasi individu berdasarkan profil DNA STR. Bercak darah merupakan barang bukti utama dalam kejadian kriminal. Paparan lingkungan diketahui merusak DNA, oleh karena itu penelitian ini menganalisis pengaruh lingkungan terhadap marka STR untuk kepentingan forensik. Bercak darah dari 12 individu diletakkan pada kain katun steril dikelompokkan menjadi 3 kelompok perlakuan lingkungan (dalam, luar dan tanah); 4 kelompok perlakuan lama paparan (1, 7, 14, dan 28 hari). DNA diekstraksi, dikuantifikasi menggunakan PCR real time kuantitatif, dilanjutkan amplifikasi 24 lokus STR. Stabilitas DNA diukur berdasarkan tingkat keberhasilan amplifikasi alel pada 24 lokus STR. Hasil penelitian menunjukkan hubungan bermakna (P<0.05) antara stabilitas DNA dengan pengaruh lingkungan, yang ditemukan pada lama paparan hari ke-7, 14 dan 28. Paparan lingkungan dalam tanah menunjukkan pengaruh kuat terhadap stabilitas DNA. Hasil korelasi Gamma menunjukkan paparan lingkungan memiliki tingkat korelasi yang tinggi terhadap stabilitas DNA, yaitu semakin ekstrim lingkungan dan semakin lama paparan lingkungan tersebut, stabilitas DNA semakin menurun ditunjukkan dengan terjadinya beberapa loss of heterozigosity dan allelic drop-out. Analisis pada tiap lokus menunjukkan semakin panjang fragmen alel pada lokus semakin mudah alel tersebut terdegradasi. Pada penelitian ini lokus CSF1PO (<350 pb), SE33 (360 pb) dan TPOX (250 pb) memiliki kecenderungan allelic drop-out paling tinggi.

---

Forensic DNA is an identification technique based on STR DNA profile. Blood stain is the main evidence in criminal act. Recent researches showed that environment have role to DNA degradation, therefore this research was designed to show environmental effect on DNA STR for forensic application of blood stain. Blood from 12 people dropped on sterile cottons and divided on 3 groups environmental (room, outdoor, soil). Each group has 4 sub-treatments time of environmental exposure (1, 7, 14, 28 days). DNA extracted from treated blood stain, quantified with real time qPCR, then 24 STR locus amplified on multiplex PCR. DNA stability measured based on amplification success rate of 24 STR locus. The results show that there were significant association (P<0.05) between stability DNA and environment effect. DNA on soil has the lowest stability among three environment condition. Significant differences were shown more significant at 28 days. Based on Gamma correlation analysis, the more extreme an environment and the longer DNA being exposed, the more decrease stability of DNA. It was shown by LOH and allelic drop-out. The longer allele, the more increase the rate of DNA degradation. In this research CSF1PO (<350bp), SE33 (<360bp), and TPOX (<250bp) have the lowest stability."

"From the Publisher: John Butler, bestselling author of Forensic DNA Typing, now applies his expertise on the subject of DNA analysis into an introductory textbook. Fundamentals of Forensic DNA Typing walks students step-by-step through the DNA analysis process beginning with collection of evidence at a crime scene to the statistical interpretation of the results. Also included are brief discussions of such news worthy topics as victim identification from the September 11, 2001 attacks, the identification of the remains of the Romano vs, the last Russian Royal family, and the O.J. Simpson case. New applications, such as genetic genealogy and tracing domestic pet hairs to perpetrators, are also detailed. With its clear and understandable style and extensive list of online ancillaries and study aids, this textbook will make the subject accessible to students in forensic science courses worldwide. Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect; Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A. Boxes. "Data, Notes & Applications" sections throughout; Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key."

"DNA typing -- the analysis of a biological sample for a person's genetic signature -- has led to the unprecedented exoneration of hundreds of wrongfully convicted people. And every day we hear stories about how police used DNA to capture a dangerous rapist or killer. Reading these accounts, it is hard not to think of DNA typing as an unmitigated good. Who can argue with a technology that helps catch bad guys and correct law enforcement mistakes? But there is a darker side to this story -- a version less likely to play out on dramatic television shows. In Inside the Cell, Erin Murphy shows how DNA typing can be subject to misuse, mistake, and error, and lead to a police state run amok"

"Synopsis"As a communicator and researcher engaged forensic DNA analyses, John Butler is at the pinnacle of his profession. As with the previous two volumes in this series, Butler leaves no stone unturned in the clarity of his A to Y coverage of forensic DNA interpretation. This book is bound to find its way onto the fingertips of practitioners, attorneys, and students."--Richard Saferstein, Ph.D., Chief Forensic Scientist (Retired) ".It is an excellent companion to his prior book on methodology, providing important information on interpretation of results. The book should prove valuable to all people involved in processing and interpreting results used in forensic genetics and criminal investigation."-- Bruce McCord, Ph.D., Professor, Florida International University "Advanced Topics in DNA Typing/Interpretation is Dr. John Butler's best book yet in his critically acclaimed series of DNA text books..."-- Stephen Patrick Hogan Adjunct Associate Professor of Biological Science and Criminal Justice, State University of New York at Albany Read less"