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"Latar belakang: Aloe vera adalah tanaman yang telah banyak digunakan sebagai obat tradisional, salah satunya dalam penyembuhan ulserasi. Namun, belum ada penelitian yang menguji pengaruh bagian kulit Aloe vera dalam penyembuhan ulserasi mukosa mulut.Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit Aloe vera dalam mempercepat penyembuhan ulserasi mukosa mulut.Metode: Penelitian ini menggunakan 12 ekor tikus Sprague Dawley, yang dibagi dalam 4 kelompok secara acak, yaitu kelompok kontrol (3 ekor), kelompok perlakuan ekstrak kulit Aloe vera 6.25% (3 ekor), kelompok perlakuan ekstrak kulit Aloe vera 12.5% (3 ekor), dan kelompok perlakuan ekstrak kulit Aloe vera 25% (3 ekor). Daerah aplikasi adalah mukosa labial rahang bawah. Pada hari pertama kelompok kontrol dan perlakuan diaplikasikan H2O2 10% 3 X 5 menit dengan interval 5 menit, setiap hari selama 3 hari berturut-turut untuk menimbulkan ulserasi. Pada hari ke-4, 5 dan 6, kelompok kontrol diaplikasikan NaCl 0.9%, kelompok perlakuan diaplikasikan ekstrak kulit Aloe vera sebanyak 3 X 5 menit, dengan interval 90 menit. Pada hari ke-7 dan ke-9, satu ekor tikus dari tiap-tiap kelompok dimatikan dan dibuat sediaan mikroskopiknya, sementara sisanya tetap diberi perlakuan. Pada hari ke-11 seluruh sisa tikus dimatikan dan dibuat sediaan mikroskopiknya. Penilaian dilakukan secara mikroskopik dengan menggunakan metode skoring modifikasi Schlossberg A dan Ferigino PD serta Eda dan Fukuyama.Hasil: Ekstrak kulit Aloe vera konsentrasi 6.25%, 12.5%, dan 25% dapat menurunkan tingkat peradangan ulserasi mukosa mulut, sehingga mempercepat proses penyembuhannya. Secara statistik, uji Mann-Whitney pada tiap kelompok menunjukkan perbedaan yang bermakna (p<0.05).Kesimpulan: Berdasarkan penelitian, dapat disimpulkan bahwa ekstrak kulit Aloe vera konsentrasi 6.25% adalah konsentrasi terkecil yang efektif dalam mempercepat penyembuhan ulserasi mukosa mulut.

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Background: Aloe vera has been known as traditional treatment for many health problems, one of them is for ulceration healing. However, there has not been a research about the effect of outer leaf of Aloe vera on oral mucous ulceration healing.Objectives: This research?s intention is to find out the effect of Aloe vera outer leaf extract on Healing acceleration of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were used in this research, and divided into 4 groups: control group with NaCl 0.9% (3 rats), concentration 6.25% group (3 rats), concentration 12.5% group (3 rats), and concentration 25% group (3 rats). The application area is mandible labial oral mucous. On the first day, each rat received application of hydrogen peroxide 10% 3 x 5 minutes, with 5 minutes interval, for 3 days to create the ulceration. On day 4th, 5th and 6th, each group received their own application 3 x 5 minutes, with 90 minutes interval. On day 7th and 9th one of the rat in each group were taken and the microscopic preparatory were made, while the rest of the rats were receiving the same application. On the day 11th, All the rats remain were taken and the microscopic preparatory were made. Microscopic examination was done by scoring using modification of Schlossberg & Ferigino and Eda. S & Fukuyama methods.Result: Aloe vera outer leaf extract 6.25%, 12.5%, and 25%, reduce the inflammation, thus accelerate the healing process of oral mucous ulceration. Statistically with Mann-Whitney test showed that there are significant differences among each group (p<0.05).Conclusion: Based on this research, it can be concluded that Aloe vera outer leaf extract 6.25% is the lowest concentration that is effective for accelerating oral mucous inflammation."

"ABSTRACTAloe Vera is one of plant that can be used as food and traditionaltreatment. In traditional treatment, this plant has effect on healingacceleration of oral mucous ulceration. In dentistry, this plant can be used as oral mucous ulceration treatment. The aim of this research is to examine the effective dose of Aloe Vera inner leaf extract on healing acceleration of oral mucous ulceration. Twelve Sprague Dawley rats were used in this research, and divided into 2 groups, 3 rats to control group and 9 rats to treatment group. Each rat received application of hydrogen peroxide 10% to create ulceration. Control group received application of NaCl 0.9% for 3,5,7 days. Treatment group received application 6.25%, 12.5%, and 25% Aloe Vera Inner Leaf Extract for 3,5,7 days. Wound healing was observed by in histological method with light microscope, and then was scored using stages of inflammation score of Schlossberg & Ferigino and Eda. S & Fukuyama methods. Statistical analysis with Mann Whitney and Kruskal Wallis methods showed that Aloe Vera inner leaf extract 25% is the most effective concentration on healing acceleration of oral mucousulceration (p< 0,05). "

"Pendahuluan: Aloe vera adalah tanaman yang telah banyak digunakan sebagai obat tradisional, salah satunya adalah untuk pengobatan ulserasi. Aloe vera mengandung bahan-bahan anti inflamasi yang berperan dalam proses penyembuhan.Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak campuran kulit dan daging Aloe vera dalam mempercepat proses penyembuhan ulserasi mukosa mulut.Metode: Dalam penelitian ini digunakan 12 ekor tikus Sprague Dawley, yang dibagi dalam 4 kelompok secara acak, yaitu kelompok kontrol yang diaplikasi larutan NaCl 0,9% (3 ekor), kelompok perlakuan yang diaplikasi ekstrak campuran kulit dan daging Aloe vera 6.25% (3 ekor), 12.5% (3 ekor), dan 25% (3 ekor). Pada hari pertama kelompok kontrol dan perlakuan diaplikasi larutan H2O2 10% sebanyak 3 X 5 menit dengan interval 5 menit, selama 3 hari berturut-turut pada daerah vestibulum mukosa labial inferior. Pada hari ke-4, 5 dan 6, kelompok kontrol diaplikasi NaCl 0.9% dan kelompok perlakuan diaplikasi ekstrak kulit dan daging Aloe vera sebanyak 3 X 5 menit, dengan interval 90 menit. Pada hari ke-7 dan 9 satu ekor tikus dari tiap-tiap kelompok dimatikan dan dibuat sediaan mikroskopik, sementara sisanya tetap diberi perlakuan. Pada hari ke-11 seluruh sisa tikus dimatikan dan dibuat sediaan mikroskopik. Penilaian dilakukan secara histologik dengan menggunakan kombinasi skor radang menurut Schlossberg A. dan Eda S.Hasil: Ekstrak campuran kulit dan daging Aloe vera 6.25%, 12.5% dan 25% dapat menurunkan tingkat radang pada ulserasi mukosa mulut. Secara statistik, uji Mann Whitney pada tiap kelompok menunjukkan perbedaan bermakna (p< 0.05).Kesimpulan: Berdasarkan hasil penelitian diperoleh bahwa aplikasi ekstrak campuran kulit dan daging Aloe vera 6.25% selama 3 hari adalah konsentrasi terendah dan waktu tercepat dalam penyembuhan ulserasi mukosa mulut.

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Introduction: Aloe vera has been known as a herbal plant that is used to treat many health problems like mucous ulceration. The whole leaf Aloe vera consists of some anti inflammatory agents that contribute on healing process.Objective: The aim of this study is to examine the effect of the whole leaf Aloe vera extract on accelerate healing of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were used in this research, and divided into 4 groups: control group applied with Natrium Chloride solution 0.9% (3 rats), treatment group applied with the whole leaf Aloe vera extract 6.25% (3 rats), 12.5% (3 rats), and 25% group (3 rats). On the first day, Hydrogen Peroxide solution 10% is applied in each rat for 3 x 5 minutes on the vestibulum of labial inferior mucous. This method is continued for 3 days. On day 4th, 5th and 6th, each group received its own application 3 x 5 minutes, with 90 minutes interval. On day 7th and 9th, one rat in each groups were sacrificed and the microscopic slide were made, while other remain treated. On the day 11th, all the rats were sacrificed and the microscopic slides were made. Histological examination was done using combined score of Schlossberg A. and Eda S?s methods.Result: The whole leaf Aloe vera extract 6.25%, 12.5% and 25% could reduce inflammation on oral mucous ulceration. Statistically with Mann Whitney test showed that there is significant differences among each group (p<0.05).Conclusion: Application of the whole leaf Aloe vera extract 6.25% for 3 days is the lowest concentration and the shortest time in healing process of oral mucous ulceration."

"Mucous membrane pemphigoid (MMP) also known as cicatricial pemphigoid is a chronic autoimmune subepithelial blistering disease primarily affecting people over 50 years old. MMP may affect any mucosal surface which is particularly oral mucosal, conjungtiva, esophagus, or larinx is involved. MMP rarely occurs in children and adolescents, but several cases of MMP affecting 2 to 18 years old patients have been reported in previous studies. In this paper we reported a case of MMP in an eighteen year old male patient who had been diagnosed with oral mucous membrane pemphigoid by anamnesis, clinical feature, histopathology. The patient was treated with systemic corticosteroid, topical corticosteroid, oral rinse and multivitamins. Until recently corticosteroid remains as the most effective and the drug of choice to treat pemphigoid lesions. Clinicians need to pay attention in using this drug because of its potential side effect."

"Latar Belakang. Pentingnya peranan protektif keratinized mucosa (KM) yang adekuat (>2mm) di sekitar implan sebagai soft tissue seal dalam menjaga barrier terhadap penetrasi bakteri yang dapat menyebabkan penyakit peri-implant masih menjadi perdebatan karena adanya inkonsistensi hasil. Tujuan. Untuk mengetahui hubungan lebar mukosa berkeratin di sekitar implan dengan brushing discomfort (BD) dan kesehatan jaringan peri-implan secara klinis dan radiografis. Metode. Penelitian cross-sectional ini mengevaluasi 17 pasien dengan 31 implan di klinik spesialis periodonsia RSKGM FKG UI. Sampel di bagi menjadi kelompok KM adekuat dan inadekuat. Pasien di periksa secara klinis dengan skor mPI, DI dan GI pada bagian bukal permukaan implan, radiografis periapkcal untuk mendapatkan data marginal bone level (MBL). BD di nilai menggunakan visual analogue scale (VAS). Hasil. Perbandingan antar dua kelompok dengan skala numerik di uji dengan independent t-test. Terdapat perbedaan skor mPI (0,01), DI (0,03) dan GI (0,05) di antara dua kelompok. Sedangkan untuk brushing discomfort (0,88) dan MBL (0,46) tidak terdapat perbedaan yang signifikan. Kesimpulan. Inadekuat KMberkaitan dengan peningkatan akumulasi plak, debris dan inflamasi mukosa di sekitar implan.

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Background. The importance of the protective role of adequate keratinized mucosa (KM) around an implant as a soft tissue seal in maintaining a barrier against bacterial penetration that can cause peri-implant disease is still being debated due to inconsistentresults. Aim. To determine the relationship between the width of the keratinized mucosa around the implant with brushing discomfort and peri-implant tissue clinically and radiographically. Method. This cross-sectional study evaluates 17 patients (31implants) at the periodontic specialist clinic, RSKGM FKG UI. The sample group was divided into adequate and inadequate KM groups. Patients were clinically examined using score mPI, DI and GI on the buccal site of the implant, and periapical radiographs were taken to evaluate marginal bone level (MBL). Brushing discomfort was assessed using a visual analogue scale (VAS). Results. The comparison between the two groups with a numerical scale was tested using an independent t-test. There were significant differences in (0.01), DI (0.03), and GI (0.05) between the two groups. However, there was no significant difference for brushing discomfort (0.88) and MBL (0.46). Conclusion. Inadequate KM is associated with increased accumulation of plaque,debris, and mucosal inflammation around the implant."

"Latar Belakang: Perubahan genetik pada p53 menyebabkan imortalisasi dan kecenderungan sel bertransformasi menjadi neoplasma. Imortalisasi ini berhubungan dengan pemeliharaan panjang telomer oleh telomerase. hTERT adalah komponen kunci telomerase yang aktivitasnya ditekan oleh p53.Tujuan: Menganalisis profil protein hTERT pada sel galur KSSRM HSC-3 dan HSC-4 serta jaringan mukosa mulut normal. Metode: Profil protein hTERT dianalisis menggunakan teknik SDS-PAGE dan Gel Doc, Quantity One.Hasil: Protein hTERT diekspresikan oleh sel galur KSSRM mulut tipe HSC 3 dan HSC 4 serta 2 dari 17 sampel protein jaringan mukosa mulut normal.Simpulan: Protein hTERT yang diekspresikan oleh sel galur KSSRM berhubungan dengan kondisi mutan p53. Adanya ekspresi protein hTERT pada jaringan mukosa mulut normal diperkirakan karena adanya sel keratinosit dan infiltrasi sel hematopoietik.

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Backround: Genetic alteration on p53 allows cellular immortalization and predisposes cells to neoplastic transformation. This immortalization is related to telomere length maintenance by telomerase. hTERT is a key component of telomerase, which activity is suppressed by p53.Objectives: To analyze the hTERT protein profile in HSC-3 and HSC-4 OSCC cell lines and normal human oral mucosa tissue. Methods: SDS-PAGE and Gel Doc, Quantity One were used for analyzing hTERT protein profile.Results: hTERT protein expressed in HSC-3 and HSC-4 OSCC cell lines and 2/17 protein samples of normal human oral mucosal tissues.Conclusion: hTERT protein that was expressed by OSCC cell lines is related to their status of mutant p53. The existing of hTERT protein on normal human oral mucosas tissue may be caused by keratinocyte cells and infiltrated hemapoietic cells."

"One of the traditional plants that has been broadly known as the healthand healing plants is Aloe vera. It is often use to treat burns, wounds, abrasions, skin disease, alopecia, and irritation. It is further more used as a hair washed to promote hair growth and as general cosmetics to improve the complexion and to smoothen the skin. The aim of the study was to examine and compare the reaction of fresh Aloe vera and distillate Aloe vera on the inflammed oral mucosa caused by hydrogen peroxide 10%. 14 rats were used in this research and divided into 4 groups; fresh Aloe vera group (4rats), distillate Aloe vera group (4 rats), control group I (3 rats), and control group II (3 rats). All rats in each group received 9x10 minutes application of hydrogen peroxide 10% on their vestibulum mucosa, for 3 days, except rats in control group I received application of aquadest. On the 4th day, all rats in each group received 9x5 minutes with their own group's material for 3 days. The histological examination was done by scoring the density of lymphocytes in the inflammation area. Statistical result shows that there are differences of oral mucosa reactions between distillate Aloe group and fresh Aloe group. Fresh Aloe group shows the decrease of lymphocytes cell higher than distillate Aloe group. Based on this research, it can be concluded that application with fresh Aloe vera can cure oral mucosa inflammation cause by hydrogen peroxide 10% than application with distillate Aloe vera."

"Oral mucous inflammation is common in our community. Therefore we need an anti-inflammatory medicine with affordable price, relative low adverse effects, and easily obtained in our environment. Sweet fennel is a plant that widely used in our community as a decorating plant, food flavouring and herbal medicine for various diseases. The affectivity for fennel has not been clinically examined. Therefore we are interested in finding our the affectivity of sweet fennel as an alternative anti-inflammatory. The aim of this research is to examine and compare reaction of both crushed and distilled fennel fruit on inflamed oral mucosa caused by hydrogen peroxide 10%. 14 rats were used and divided into 4 groups, and controls (6 rats). They received hydrogen peroxide 10% applications on vestibulum mucosa for there days to make the inflammation. After that they received crushed and distilled fennel. There days later they were killed and the vestibulum mucosa was excised for microscopic slides. The results show that the crushed the redness of oral mucosa. The conclusion is fennel could reduce an inflammation and there is no difference of healing either by crushed or distilled fennel."

"Latar Belakang: p73, homolog p53, diketahui memiliki kemampuan serupa dalam menekan pertumbuhan tumor. Protein p73 diekspresikan dalam berbagai level pada sel kanker dan jaringan normal yang berbeda. Belum diketahui bagaimana pola ekspresi protein p73 pada KSSRM dan pada jaringan mukosa mulut normal.Tujuan: Mengetahui profil protein p73 pada KSSRM tipe mutant p53 dan jaringan mukosa mulut normal berdasarkan berat molekul protein.Metode: Ekstrak protein dari HSC-3 dan HSC-4 serta jaringan mukosa normal dianalisa dengan teknik SDS PAGE untuk mendeteksi protein p73 berdasarkanberat molekulnya.Hasil:. pita protein p73 pada HSC-3 lebih tebal daripada HSC- 4. Terdapat variasi profil protein p73 pada mukosa mulut normal dengan pita protein tebal (8/17) dan sedang (5/17).Simpulan: Terdapat perbedaan profil protein p73 antara HSC-3 dan HSC-4 berkaitan dengan tingkat protein p53 dan SNP pada kodon 72. Kebanyakan sampel jaringan mukosa memperlihatkan ketebalan pita protein p73 yang cukup tinggi.

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Backround: p73, the homolog of p53, has a similar ability in tumor suppression. p73 protein expressed at a different level in various cancer cells and normal tissues. Profile of p73 protein in mutant p53 OSCC cell line and normal human oral mucosa have not been known.

Objectives: To observe p73 protein profile in mutant p53 OSCC cell lines and normal human oral mucosa.

Methods: The extracted protein of HSC-3 and HSC-4 cell lines and normal mucosal tissues were analyzed with SDS PAGE to detect p73 protein based on the molecular weight.

Results:. The band of p73 protein in HSC-3 shows a higher density compared to its density of HSC-4. A thick p73 protein band was shown on 8/17 of normal mucosal tissues while medium level of p73 protein band was shown on 5/17.

Conclusion: The protein profile between HSC-3 and HSC-4 were different related with p53 protein and SNP on codon 72 of each samples. Most of mucosal tissues shows a quite high density of p73 protein bands."