Penelitian ini mengkaji pengaruh intensitas pencahayaan (3000, 5000, dan 10000 lux) serta jenis kultur mikroalga mikroalga (Spirulina platensis, Chlorella vulgaris, dan konsorsium bioaktif) terhadap produksi biomassa dan kandungan bioaktif. Rancangan penelitian menggunakan metode faktorial 3×3 yang dianalisis dengan Two-Way ANOVA dan uji Tukey HSD. Hasil menunjukkan bahwa pencahayaan dan jenis kultur mikroalga berpengaruh signifikan (p < 0,0001) terhadap seluruh parameter, meliputi biomassa, laju pertumbuhan, klorofil, fikosianin, protein, lipid, dan karbon organik. Biomassa tertinggi dicapai oleh C. vulgaris pada 5000 lux, sedangkan produktivitas harian tertinggi diperoleh dari konsorsium pada intensitas yang sama. Kandungan klorofil tertinggi tercatat pada konsorsium 50%:50%, sementara fikosianin paling tinggi dihasilkan oleh S. platensis. Konsorsium dengan intensitas 10000 lux menghasilkan kandungan protein dan karbon organik tertinggi, sementara lipid tertinggi berasal dari S. platensis pada 10000 lux. Temuan ini menunjukkan bahwa sistem kultur campuran dan pencahayaan optimal dapat meningkatkan efisiensi metabolik mikroalga, sehingga berpotensi diterapkan dalam produksi bioenergi, pangan fungsional, dan biofarmasi.

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This study investigates the effect of light intensity (3000, 5000, and 10000 lux) and inoculum composition (Spirulina platensis, Chlorella vulgaris, and a 50%:50% consortium) on microalgal biomass production and bioactive compound accumulation. A 3×3 factorial experimental design was applied and analyzed using Two-Way ANOVA and Tukey HSD tests. The results revealed that both light intensity and inoculum composition significantly affected (p < 0.0001) all measured parameters, including biomass, specific growth rate, chlorophyll, phycocyanin, protein, lipid, and organic carbon content. The highest biomass was obtained from C. vulgaris at 5000 lux, while the highest daily productivity was recorded in the consortium under the same intensity. The highest chlorophyll content was found in the 50%:50% consortium, whereas the highest phycocyanin content was produced by S. platensis. The consortium under 10000 lux yielded the highest protein and organic carbon content, while the highest lipid content was produced by S. platensis at 10000 lux. These findings indicate that mixed cultures and optimized light conditions can enhance microalgal metabolic efficiency, suggesting potential applications in bioenergy, functional food, and biopharmaceutical industries.