Artikel Jurnal :: Kembali

Artikel Jurnal :: Kembali

Pengaruh media dasar ms dan n6 terhadap perkembangan embrio somatik pada kultur meristem jahe (zingiber officinale rosc.): The Effect of MS and N6 Basal Media to Somatic Embryo Development in Meristematic Culture of Ginger (Zingiber officinale Rosc.)

Sitti Fatimah Syahid (Pusat Penelitian Biologi, 2007)

 Abstrak

This study was performed to evaluate the development of somatic embryo from embryogenic calli of ginger meristem culture. Completely randomized design was applied, replicated 4 times. Embryogenic calli from meristem tissue of inner shoot bud of rhizome obtained on MS medium containing 100 mg/L glutamine, 2% sucrose with the addition of 1.0 mg/L 2,4-D and 3.0 mg/L BA, were subjected to proliferation medium, MS and N basal media containing 3% mannitol. Then, transferred into somatic embryo maturation medium, either MS or N basal media supplemented with 6% sucrose. The number of somatic embryos-formed significantly affected by the proliferation medium applied. The highest number of somatic embryos (about 82.0 per 1 g friable calli) was achieved on the MS medium, 4 weeks after incubation. In addition, the optimum growth of embryogenic calli containing somatic embryos was obtained on MS and N medium supplemented with 6% sucrose. There were significantly difference between the media applied (MS and N ) to somatic embryos maturation. The highest number of mature somatic embryos (57.2 embrios) was achieved on the MS medium, 18 days after incubation.

 Metadata

Jenis Koleksi : Artikel Jurnal
No. Panggil : BBIO 8:5 (2007)
Entri utama-Nama orang :
Subjek :
Penerbitan : Bogor: Pusat Penelitian Biologi, 2007
Sumber Pengatalogan : LibUI ind rda
ISSN : 01261754
Majalah/Jurnal : Pusat Penelitian Biologi
Volume : Vol. 8, No. 5, Agustus 2007: Hal. 343-352
Tipe Konten : text
Tipe Media : unmediated
Tipe Carrier : volume
Akses Elektronik : https://media.neliti.com/media/publications/53155-ID-none.pdf
Institusi Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 4 R. Koleksi Jurnal
  • Ketersediaan
  • Ulasan
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No. Panggil No. Barkod Ketersediaan
BBIO 8:5 (2007) 08-25-30619619 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 9999920567356
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