Teknik rekayasa jaringan kini dikembangkan untuk perawatan kerusakan tulang yang besar. Pada kasus one wall defect dibutuhkan scaffold dalam bentuk membran yang dikombinasikan dengan RGD untuk memfasilitasi regenerasi jaringan. Tujuan: Mengetahui efek penambahan RGD kepada scaffoldmembran kitosan terhadap proliferasi sel pulpa manusia. Metode: Scaffold membran kitosan kulit udang RGD dipaparkan kepada sel pulpa manusia hasil primary culture dan diuji menggunakan MTT-assay. Hasil: Terdapat peningkatan proliferasi sel pulpa manusia yang bermakna pada kelompok scaffold membran kitosan kulit udang RGD dibandingkan dengan kelompok kontrol. Kesimpulan:Scaffold membran kitosan kulit udang RGD mampu meningkatkan proliferasi sel pulpa manusia.

---

Background: Tissue engineering is now being developed to treat large bone defect. A membrane scaffold with addition of RGD is needed to treat one wall defect as it is capable to fasilitate tissue regeneration.

Objective: To analyze the effect of RGD addition to shrimp shells chitosan scaffold membrane on human dental pulp cell proliferation.

Methods: Human dental pulp cell was exposed by shrimp shells chitosan membrane scaffold with RGD addition and was tested using MTT assay.

Result: Proliferation of human dental pulp cell exposed by shrimp shells chitosan membrane scaffold RGD shows a significant increase compared to control.

Conclusion: Shrimp shells chitosan scaffold membrane RGD can increase human dental pulp cell proliferation.