UI - Tesis (Membership) :: Kembali

UI - Tesis (Membership) :: Kembali

Efek mangiferin terhadap kerusakan otak akibat stres oksidatif dan inflamasi yang ditimbulkan oleh pemberian doksorubisin = The effect of mangiferin against brain damage caused by oxidative stress and inflammation induced by doxorubicin / Soni Siswanto

Soni Siswanto; Wawaimuli Arozal, supervisor; Vetnizah Juniantito, examiner; Anggi Gayatri, examiner; Sri Widia Jusman, examiner; Radiana Dhewayani Antarianto, examiner (, 2015)
 Abstrak
[ABSTRAK

Latar belakang. Doksorubisin (DOK), suatu antibiotika antrasiklin, digunakan
secara luas untuk terapi antikanker, namun penggunaan DOK dapat menimbulkan
efek samping, salah satunya gangguan kognitif. Penggunaan kemoterapi berbasis
DOK menunjukkan hingga 76% pasien mengalami penurunan kognitif.
Kerusakan otak akibat penggunaan DOK disebabkan oleh peningkatan TNF-α di
otak melalui uptake reseptor di sawar darah otak dan peningkatan produksi
melalui aktivasi NF-κB. Peningkatan TNF-α lebih lanjut dapat menyebabkan
inflamasi kronis yang dapat menimbulkan kematian sel saraf atau penyakit
degenerasi saraf. Mangiferin (MAG) merupakan salah satu senyawa
neuroprotektif, akan tetapi efek terhadap kerusakan otak akibat pemberian DOK
belum diketahui. Penelitian ini bertujuan untuk mengetahui efek MAG terhadap
kerusakan otak yang ditimbulkan oleh pemberian DOK.
Metode. Penelitian dilakukan terhadap tikus Sprague-Dawley yang diinduksi
menggunakan DOK dengan dosis total 15 mg/kgBB secara i.p mulai minggu
kedua. Pemberian MAG dilakukan secara p.o dengan dosis 30 dan 60 mg/kgBB
selama 7 minggu. Parameter yang diamati adalah fungsi kognitif, inflamasi (TNF-
α, NF-κB dan iNOS), stres oksidatif (SOD dan MDA) dan histopatologi dengan
pewarnaan HE.
Hasil. Pemberian DOK menyebabkan gangguan kognitif yang ditandai dengan
penurunan penggiliran labirin Y dan penurunan indeks diskriminasi pada
pengenalan obyek baru, disertai peningkatan parameter inflamasi yaitu ekspresi
TNF-α, NF-κB dan iNOS. Pemberian MAG bersama DOK menyebabkan
peningkatan fungsi kognitif, penurunan inflamasi dan penurunan stres oksidatif
serta histopatologi dewan pewarna HE.
Kesimpulan. Berdasarkan hasil pemeriksaan parameter pada penelitian
mengindikasikan bahwa mangiferin memiliki efek neuroproteksi terhadap
pemberian DOK.


ABSTRACT

Introduction. Doxorubicin (DOK), an anthracycline antibiotic, is widely used for
anticancer therapy, but the use of DOK causing side effects, one of them is
cognitive impairment. Up to 76% of patients experienced cognitive decline caused
by DOK-based chemotherapy. Brain damage due to the use of DOK lead by an
increase in TNF-α in the brain through the receptors uptake in the blood brain
barrier and increasing production through activation of NF-κB. Increased TNF-α
can further lead to chronic inflammation which can lead nerve cells death or nerve
degeneration diseases. Mangiferin (MAG) is one of the neuroprotective
compound, but the effect on brain damage induced by DOK is still unknown. This
study aims to determine the effect of MAG on brain damage induced by DOK.
Methods. Research carried out on Sprague-Dawley rats induced by DOK i.p with
total dose 15 mg/kg that divided into 6 dose and given within 2 weeks, started
from 2nd week. The rats was administrated by MAG p.o with dose 30 and 60
mg/kg daily for 7 weeks. Parameters measured were cognitive function,
inflammatory parameters (TNF-α, NF-κB and iNOS), oxidative stress parameters
(SOD and MDA) and histopatology using HE staining.
Results. DOK cause cognitive disorders that characterized by decreased Y maze
alteration and discrimination index in new object recognition, and accompanied
by increasing inflammatory parameters that showed in increasing TNF-α, NF-κB
and iNOS expressions. Coadministration MAG with DOK led an increasing on
cognitive function, reducing the inflammation and oxidative stress.
Conclusion. Based on the results of the study, MAG indicated has a
neuroprotective effect on brain damage induced by DOK;Introduction. Doxorubicin (DOK), an anthracycline antibiotic, is widely used for
anticancer therapy, but the use of DOK causing side effects, one of them is
cognitive impairment. Up to 76% of patients experienced cognitive decline caused
by DOK-based chemotherapy. Brain damage due to the use of DOK lead by an
increase in TNF-α in the brain through the receptors uptake in the blood brain
barrier and increasing production through activation of NF-κB. Increased TNF-α
can further lead to chronic inflammation which can lead nerve cells death or nerve
degeneration diseases. Mangiferin (MAG) is one of the neuroprotective
compound, but the effect on brain damage induced by DOK is still unknown. This
study aims to determine the effect of MAG on brain damage induced by DOK.
Methods. Research carried out on Sprague-Dawley rats induced by DOK i.p with
total dose 15 mg/kg that divided into 6 dose and given within 2 weeks, started
from 2nd week. The rats was administrated by MAG p.o with dose 30 and 60
mg/kg daily for 7 weeks. Parameters measured were cognitive function,
inflammatory parameters (TNF-α, NF-κB and iNOS), oxidative stress parameters
(SOD and MDA) and histopatology using HE staining.
Results. DOK cause cognitive disorders that characterized by decreased Y maze
alteration and discrimination index in new object recognition, and accompanied
by increasing inflammatory parameters that showed in increasing TNF-α, NF-κB
and iNOS expressions. Coadministration MAG with DOK led an increasing on
cognitive function, reducing the inflammation and oxidative stress.
Conclusion. Based on the results of the study, MAG indicated has a
neuroprotective effect on brain damage induced by DOK, Introduction. Doxorubicin (DOK), an anthracycline antibiotic, is widely used for
anticancer therapy, but the use of DOK causing side effects, one of them is
cognitive impairment. Up to 76% of patients experienced cognitive decline caused
by DOK-based chemotherapy. Brain damage due to the use of DOK lead by an
increase in TNF-α in the brain through the receptors uptake in the blood brain
barrier and increasing production through activation of NF-κB. Increased TNF-α
can further lead to chronic inflammation which can lead nerve cells death or nerve
degeneration diseases. Mangiferin (MAG) is one of the neuroprotective
compound, but the effect on brain damage induced by DOK is still unknown. This
study aims to determine the effect of MAG on brain damage induced by DOK.
Methods. Research carried out on Sprague-Dawley rats induced by DOK i.p with
total dose 15 mg/kg that divided into 6 dose and given within 2 weeks, started
from 2nd week. The rats was administrated by MAG p.o with dose 30 and 60
mg/kg daily for 7 weeks. Parameters measured were cognitive function,
inflammatory parameters (TNF-α, NF-κB and iNOS), oxidative stress parameters
(SOD and MDA) and histopatology using HE staining.
Results. DOK cause cognitive disorders that characterized by decreased Y maze
alteration and discrimination index in new object recognition, and accompanied
by increasing inflammatory parameters that showed in increasing TNF-α, NF-κB
and iNOS expressions. Coadministration MAG with DOK led an increasing on
cognitive function, reducing the inflammation and oxidative stress.
Conclusion. Based on the results of the study, MAG indicated has a
neuroprotective effect on brain damage induced by DOK]
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No. Panggil : T-Pdf
Pengarang :
Pengarang/kontributor lain :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2015
Program Studi :
Naskah Ringkas :
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xvii, 107 pages : illustration ; 28 cm + appendix
Catatan Bibliografi : pages 57-66
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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