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Potensi ekstrak metanol akar pasak bumi (eurycoma longifolia jack) sebagai imunomodulator mencit putih jantan (mus musculus) terhadap vaksin BCG = The potency of methanol extract of pasak bumi root (eurycoma longifolia jack) as immunomodulators in male white mice (mus musculus) toward the BCG vaccine

Kensarita; Andi Yasmon, supervisor; Heri Wibowo, supervisor; Beti Ernawati Dewi, examiner; Mohamad Sadikin, examiner; Erni Hernawati Purwaningsih, examiner (, 2014)
 Abstrak
[ABSTRAK

Kemampuan Mycobacterium tuberculosis yang dapat memanipulasi dan
menghindari respon imun adalah tantangan dalam mencari terapi dan vaksinasi
efektif. Penelitian ini untuk menguji potensi ekstrak metanol akar Eurycoma
longifolia Jack dalam memodulasi peningkatan IgA dan IgG mencit putih jantan
yang divaksinasi BCG, sekaligus untuk menganalisis peningkatan sitokin
proinflamasi yang berhubungan dengan produksi IgA dan IgG. Plasma diambil
sebelum dan setelah vaksinasi BCG pada 18 ekor mencit yang dibagi ke dalam 2
kelompok; perlakuan dan kontrol. Kadar IgA, IgG, TNF-! dan IL-10 diukur
dengan metode ELISA. Hasil menunjukkan bahwa peningkatan IgA kelompok air
(0,33±0,16) lebih tinggi dibandingkan kelompok pasak bumi (0,30±0,30),
sedangkan peningkatan IgG kelompok pasak bumi (0,38±0,25) terlihat lebih
tinggi dibandingkan kelompok air (0,29±0,35). Rasio TNF-!/IL-10 kelompok
pasak bumi (0,46±0,07) lebih tinggi dibandingkan kelompok air (0,41±0,05).
Terdapat korelasi antara TNF-! dengan IgA pada kelompok air (r=0,601,
p=0,035) juga dengan IgG pada kelompok pasak bumi (r=0,559, p=0,059).
Disimpulkan, pemberian pasak bumi cenderung berpotensi memodulasi
peningkatan IgG, tetapi tidak IgA. Selain itu, pemberian pasak bumi juga
cenderung meningkatkan sitokin proinflamasi yang mempengaruhi produksi IgA
dan IgG.


ABSTRACT

The ability of Mycobacterium tuberculosis to manipulate and evade host?s
immune response is a challenge in finding an effective therapy and vaccination.
The purpose of this research were to investigate the potency of methanol extract
of the roots of Eurycoma longifolia Jack as immunomodulators for male white
mice vaccinated with BCG, as well as to analyze the increase of proinflammatory
cytokines associated with IgA and IgG production. Plasma were taken before and
after BCG vaccination from 18 mice, that were divided into 2 groups; treatment
and control. IgA, IgG, TNF-! and IL-10 levels were measured by using ELISA.
The results showed that the enhancement of IgA group with water (0,33±0,16)
was higher than group with pasak bumi (0,30±0,30). On the other hand the
enhancement of IgG group with pasak bumi (0,38±0,25) was higher than group
with water (0,29±0,35). The ratio of TNF-!/IL-10 group with pasak bumi
(0.46±0.07) was higher than group with water (0.41±0.05). There are correlarions
between TNF-! and IgA in the group with water (r=0.601, p=0.035) as well as
between TNF- ! and IgG in group with pasak bumi (r=0.559, p=0.059). As the
conclusion, methanol extract of pasak bumi root tend to potentially modulate the
increase IgG production, but not IgA. In addition, it also tends to increase the
proinflamasi cytokines associated with IgA and IgG production., The ability of Mycobacterium tuberculosis to manipulate and evade host’s
immune response is a challenge in finding an effective therapy and vaccination.
The purpose of this research were to investigate the potency of methanol extract
of the roots of Eurycoma longifolia Jack as immunomodulators for male white
mice vaccinated with BCG, as well as to analyze the increase of proinflammatory
cytokines associated with IgA and IgG production. Plasma were taken before and
after BCG vaccination from 18 mice, that were divided into 2 groups; treatment
and control. IgA, IgG, TNF-! and IL-10 levels were measured by using ELISA.
The results showed that the enhancement of IgA group with water (0,33±0,16)
was higher than group with pasak bumi (0,30±0,30). On the other hand the
enhancement of IgG group with pasak bumi (0,38±0,25) was higher than group
with water (0,29±0,35). The ratio of TNF-!/IL-10 group with pasak bumi
(0.46±0.07) was higher than group with water (0.41±0.05). There are correlarions
between TNF-! and IgA in the group with water (r=0.601, p=0.035) as well as
between TNF- ! and IgG in group with pasak bumi (r=0.559, p=0.059). As the
conclusion, methanol extract of pasak bumi root tend to potentially modulate the
increase IgG production, but not IgA. In addition, it also tends to increase the
proinflamasi cytokines associated with IgA and IgG production.]
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 Metadata
No. Panggil : T-Pdf
Pengarang :
Pengarang/kontributor lain :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2014
Program Studi :
Naskah Ringkas :
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xiv, 73 pages : illustration ; 28 cm + appendix
Catatan Bibliografi : pages 41-45
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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T-Pdf 15-17-384294621 TERSEDIA
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