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UI - Skripsi (Membership) :: Kembali

Pengaruh pemberian ekstrak biji jengkol (archidendron pauciflorum) pada aktivitas spesifik katalase jaringan hati tikus yang diintoksikasi karbon tetraklorida ( CCL4 ) = The effect of jengkol seed extract (archidendron pauciflorum) on catalase specific activity of ( CCL4) induced liver injury in rats

Mochamad Faisal Adam; Mohamad Sadikin, supervisor; Ani Retno Prijanti, supervisor; Erni Hernawati Purwaningsih, examiner (, 2013)
 Abstrak
[ABSTRAK

Berbagai penyakit yang disebabkan radikal bebas semakin meningkat
khususnya di Indonesia mengingat paparan sinar ultraviolet yang cukup
banyak di daerah tropis, pembangunan yang pesat, serta adanya perubahan
gaya hidup. Oleh karena itu peran antioksidan eksogen diperlukan
untukomembantu antioksidan endogen, seperti enzim katalase, agar terhindar
dari stres oksidatif yang ditimbulkan radikal bebas. Jengkol (Archidendron
pauciflorum), salah satu tanaman tropis Indonesia, memiliki potensi
antikosidan kuat karena memiliki asam jengkolat,oyang tersusun dari dua
molekul sisteinlyangodikenal sebagai antioksidan. Selain itu jengkol juga
memiliki kandungan antioksidan lain seperti vitamin C dan flavonoid,
terutama pada bijinya. Penelitian ini dilakukan untuk mengetahui pengaruh
pemberian ekstrak biji jengkol pada aktivitas spesifik katalase jaringan hati
tikus. Sebanyak 32 ekor tikuslSprague Dawley dibagi dalam empat kelompok
secara acak, yaitu kelompok perlakuan standar, kelompok dengan pemberian
ekstrak biji jengkol, kelompok dengan pemberian CCl4 sebagai indikator
kerusakan hati, serta kelompok dengan pemberian ekstrak biji jengkol
disertailCCl4. Homogenatkhati tikus masing-masing kelompok diukur
aktivitas spesifik katalasenyaldengan metode Mates. Analisis statistik
menunjukkan bahwa ekstrak biji jengkol dapat menurunkan aktivitaslspesifik
katalase, baik pada hati tikus normal (p=0.000) maupun pada hati yang
dirusak CCl4, walaupun tidak bermakna (p=0.832).lHal tersebut diperkirakan
karena gugus sulfhidiril (SH) dari sistein yang dibebaskan dari asam
jengkolat, yang dapat menginaktivasi kerja enzim katalase.


ABSTRACT

Free radical-related disease are more increasing especially in Indonesia
because of tropical situation there such as ultraviolet and life style changes.
Exogen antioxidants are increasingly needed to help endogen antioxidants
activity, such as catalase, to avoid oxidative stress induced by free radical
exposure. One of indonesian tropical plant, Jengkol (Archidendron
pauciflorum) is believed have strong potential antioxidant source, jengkolic
acid, a compund consisting of two cysteine molecules which has been known
as antioxidants, besides, their other known sources of antioxidant: vitamin C,
and flavonoid. Research is conducted to find the effect of Jengkol seeds
extract towards specific catalase activity of rat?s liver. Thirty two Spraguedawley
strain rats are divided into four groups: control group, a group given
jengkol seeds extract, a negative control group given CCl4 to show
hepatocytes toxicity, and a group given both CCl4 and jengkol seeds extract.
Homogenate of rat liver from each groups are measured for their spesific
catalase activity using Mates methods. The result shows jengkol seeds extract
reduced specific catalase activity in normal rat liver significantly (p=0,000),
also in injuried liver by CCl4, although no significant correlation found
(p=0,832). This finding shows a possible inactivation of catalase enzyme due
to sulfhydril (SH) groups from cysteine after being released by jengkolic acid., Free radical-related disease are more increasing especially in Indonesia
because of tropical situation there such as ultraviolet and life style changes.
Exogen antioxidants are increasingly needed to help endogen antioxidants
activity, such as catalase, to avoid oxidative stress induced by free radical
exposure. One of indonesian tropical plant, Jengkol (Archidendron
pauciflorum) is believed have strong potential antioxidant source, jengkolic
acid, a compund consisting of two cysteine molecules which has been known
as antioxidants, besides, their other known sources of antioxidant: vitamin C,
and flavonoid. Research is conducted to find the effect of Jengkol seeds
extract towards specific catalase activity of rat’s liver. Thirty two Spraguedawley
strain rats are divided into four groups: control group, a group given
jengkol seeds extract, a negative control group given CCl4 to show
hepatocytes toxicity, and a group given both CCl4 and jengkol seeds extract.
Homogenate of rat liver from each groups are measured for their spesific
catalase activity using Mates methods. The result shows jengkol seeds extract
reduced specific catalase activity in normal rat liver significantly (p=0,000),
also in injuried liver by CCl4, although no significant correlation found
(p=0,832). This finding shows a possible inactivation of catalase enzyme due
to sulfhydril (SH) groups from cysteine after being released by jengkolic acid.]
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No. Panggil : S-Pdf
Pengarang :
Pengarang/kontributor lain :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2013
Program Studi :
Naskah Ringkas : Download naskah ringkas
Kode Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xiii, 51 pages : illustration ; 30 cm. + Appendix
Catatan Bibliografi : pages 47-51
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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