UI - Tesis (Open) :: Kembali

UI - Tesis (Open) :: Kembali

Pemanfaatan ekstrak centella asiatica linn dan acalypha indica linn dalam meningkatkan rekayasa sel punca mesenkim asal darah tepi untuk pendekatan terapi sel

Robi Irawan; Nurhadi Ibrahim, supervisor; Cholid Badri, examiner; Dangsina Moeloek, examiner (, 2011)
 Abstrak
[ABSTRAK

Terapi sel merupakan salah satu pendekatan penyembuhan penyakit
degenerasi yang memberikan harapan untuk dapat memperbaiki organ atau jaringan sehingga memberikan hasil yang memuaskan dalam hal regenerasi dan pengembalian fungsi normal suatu organ. Sel punca mesenkim diketemukan dalam darah manusia normal yang dapat dikultur. Sel punca mesenkim memiliki morfologi, cytoskeletal, cytoplasmik dan penanda permukaan (CD14-,CD31-, CD34-, CD44+, CD45-, CD73+, CD90+, CD105+, dan CD166+) yang sama seperti
precursor mensenkim sumsum tulang. Darah tepi merupakan sumber yang menjanjikan untuk digunakan sebagai alternatif sumber sel punca mesenkim untuk tujuan terapi sel karena memiliki keuntungan yaitu tidak invasif, mudah, tidak perlu dilakukan biopsi dan tidak memerlukan keahlian dalam mendapatkannya. Namun ada kekurangan yang dimiliki oleh sel punca mesenkim yang berasal dari darah tepi yaitu jumlah populasi lebih sedikit dibandingkan dengan populasi yang
dimiliki sel punca mesenkim yang berasal dari sumsum tulang.



Mengamati pengaruh pemberian ekstrak Centella asiatica (pegagan) dan Acalypha indica (air akar kucing) terhadap peningkatan efisiensi rekayasa sel pada kultur sel punca mesenkim asal darah tepi dalam pendekatan terapi sel.



Studi eksperimental in vitro pada kultur primer dan kultur post pasasi pada sel punca mesenkim asal darah tepi. Kelompok perlakuan terdiri atas beberapa kelompok yaitu satu kelompok control, 3 kelompok ekstrak air Acalypha indica (10mg/mL, 15mg/mL, 20mg/mL) dan 3 kelompok ekstrak air Centella asiatica
(10μg/mL,15μg/mL,20μg/mL) selama 17 hari untuk kultur primer dan 48 jam pada kultur post pasasi. Setelah diberi perlakuan, nilai viabilitas relatif sel dan tingkat proliferasi sel diukur dengan metode MTT.



Viabilitas relatif sel dan tingkat proliferasi sel pada kultur primer dan kultur post pasasi sel punca mesenkim dengan pemberian ekstrak Centella asiatica memiliki tingkat proliferasi lebih tinggi secara bermakna dibandingkan dengan kontrol dan pemberian ekstrak Acalypha indica Linn (p < 0,05).



Pemberian ekstrak Centella asiatica lebih bermanfaat dalam meningkatkan proliferasi sel dan viabilitas relatif sel dibandingkan ekstrak Acalypha indica pada kultur post pasasi PBMC yang diperlukan untuk mendapatkan sel punca mesenkim yang akan dijadikan terapi sel


ABSTRACT

Cell therapy is one of healing degeneration diseases approaching which
provides the hoping of organ or tissue repairing to provide satisfactory results in terms of regeneration and rehabilitation organ function. Mesenchymal stem cell found in the human peripheral blood. This stem cell have morphology, cytoskeletal, cytoplasmik and surface markers (CD14-, CD31-, CD34-, CD44 +, CD45-, CD73 +, CD90 +, CD105 + and CD166 +) which are the same with Bone marrow derived mesenchymal stem cell. Peripheral blood is a promising source
that can be used as an alternative source of /Mesenchymal stem cells for cell therapy because it has the advantage that are not invasive, easy to cultur, not necessary for biopsy treatment and requires no expertise to be collected. The disadvantages of Mesenchymal stem cells derived peripheral blood are less population compared to bone marrow derived mesenchymal stem cells.



This research purpose to observe the effect of Centella asiatica and
Acalypha indica extract in Mesenchymal stem cells derived peripheral blood cultured to approach cell therapy.



Experimental studies in vitro in primary culture and subculture of Mesenchymal stem cells derived peripheral blood. The treatment groups consisted of several groups: one control group, three groups of Acalypha indica water extract (10mg/mL, 15mg/mL, 20mg/mL) and three groups of Centella asiatica
water extract (10μg/mL, 15μg/mL, 20μg/mL) for 17 days primary culture and 48 hours subculture. Further treatment, the relative cell viability and cell proliferation rate are measured by MTT method.



Relative cell viability and cell proliferation rate of primary culture cells
and the Mesenchymal stem cells subculture from Centella asiatica extract have a significant higher proliferation than the control group and Acalypha indica Linn extract (p <0.05).



Centella asiatica extract is more useful for increasing cell proliferation rate and relative cell viability compared to Acalypha indica extracts in PBMC culture to obtain mesenchymal stem cells that will be used for cell.;Cell therapy is one of healing degeneration diseases approaching which
provides the hoping of organ or tissue repairing to provide satisfactory results in
terms of regeneration and rehabilitation organ function. Mesenchymal stem cell
found in the human peripheral blood. This stem cell have morphology,
cytoskeletal, cytoplasmik and surface markers (CD14-, CD31-, CD34-, CD44 +,
CD45-, CD73 +, CD90 +, CD105 + and CD166 +) which are the same with Bone
marrow derived mesenchymal stem cell. Peripheral blood is a promising source
that can be used as an alternative source of /Mesenchymal stem cells for cell
therapy because it has the advantage that are not invasive, easy to cultur, not
necessary for biopsy treatment and requires no expertise to be collected. The
disadvantages of Mesenchymal stem cells derived peripheral blood are less
population compared to bone marrow derived mesenchymal stem cells.
This research purpose to observe the effect of Centella asiatica and
Acalypha indica extract in Mesenchymal stem cells derived peripheral blood
cultured to approach cell therapy.
Experimental studies in vitro in primary culture and subculture of
Mesenchymal stem cells derived peripheral blood. The treatment groups consisted
of several groups: one control group, three groups of Acalypha indica water
extract (10mg/mL, 15mg/mL, 20mg/mL) and three groups of Centella asiatica
water extract (10μg/mL, 15μg/mL, 20μg/mL) for 17 days primary culture and 48
hours subculture. Further treatment, the relative cell viability and cell proliferation
rate are measured by MTT method.
Relative cell viability and cell proliferation rate of primary culture cells
and the Mesenchymal stem cells subculture from Centella asiatica extract have a
significant higher proliferation than the control group and Acalypha indica Linn
extract (p <0.05).
Centella asiatica extract is more useful for increasing cell proliferation rate
and relative cell viability compared to Acalypha indica extracts in PBMC culture
to obtain mesenchymal stem cells that will be used for cell;Cell therapy is one of healing degeneration diseases approaching which
provides the hoping of organ or tissue repairing to provide satisfactory results in
terms of regeneration and rehabilitation organ function. Mesenchymal stem cell
found in the human peripheral blood. This stem cell have morphology,
cytoskeletal, cytoplasmik and surface markers (CD14-, CD31-, CD34-, CD44 +,
CD45-, CD73 +, CD90 +, CD105 + and CD166 +) which are the same with Bone
marrow derived mesenchymal stem cell. Peripheral blood is a promising source
that can be used as an alternative source of /Mesenchymal stem cells for cell
therapy because it has the advantage that are not invasive, easy to cultur, not
necessary for biopsy treatment and requires no expertise to be collected. The
disadvantages of Mesenchymal stem cells derived peripheral blood are less
population compared to bone marrow derived mesenchymal stem cells.
This research purpose to observe the effect of Centella asiatica and
Acalypha indica extract in Mesenchymal stem cells derived peripheral blood
cultured to approach cell therapy.
Experimental studies in vitro in primary culture and subculture of
Mesenchymal stem cells derived peripheral blood. The treatment groups consisted
of several groups: one control group, three groups of Acalypha indica water
extract (10mg/mL, 15mg/mL, 20mg/mL) and three groups of Centella asiatica
water extract (10μg/mL, 15μg/mL, 20μg/mL) for 17 days primary culture and 48
hours subculture. Further treatment, the relative cell viability and cell proliferation
rate are measured by MTT method.
Relative cell viability and cell proliferation rate of primary culture cells
and the Mesenchymal stem cells subculture from Centella asiatica extract have a
significant higher proliferation than the control group and Acalypha indica Linn
extract (p <0.05).
Centella asiatica extract is more useful for increasing cell proliferation rate
and relative cell viability compared to Acalypha indica extracts in PBMC culture
to obtain mesenchymal stem cells that will be used for cell, Cell therapy is one of healing degeneration diseases approaching which
provides the hoping of organ or tissue repairing to provide satisfactory results in
terms of regeneration and rehabilitation organ function. Mesenchymal stem cell
found in the human peripheral blood. This stem cell have morphology,
cytoskeletal, cytoplasmik and surface markers (CD14-, CD31-, CD34-, CD44 +,
CD45-, CD73 +, CD90 +, CD105 + and CD166 +) which are the same with Bone
marrow derived mesenchymal stem cell. Peripheral blood is a promising source
that can be used as an alternative source of /Mesenchymal stem cells for cell
therapy because it has the advantage that are not invasive, easy to cultur, not
necessary for biopsy treatment and requires no expertise to be collected. The
disadvantages of Mesenchymal stem cells derived peripheral blood are less
population compared to bone marrow derived mesenchymal stem cells.
This research purpose to observe the effect of Centella asiatica and
Acalypha indica extract in Mesenchymal stem cells derived peripheral blood
cultured to approach cell therapy.
Experimental studies in vitro in primary culture and subculture of
Mesenchymal stem cells derived peripheral blood. The treatment groups consisted
of several groups: one control group, three groups of Acalypha indica water
extract (10mg/mL, 15mg/mL, 20mg/mL) and three groups of Centella asiatica
water extract (10μg/mL, 15μg/mL, 20μg/mL) for 17 days primary culture and 48
hours subculture. Further treatment, the relative cell viability and cell proliferation
rate are measured by MTT method.
Relative cell viability and cell proliferation rate of primary culture cells
and the Mesenchymal stem cells subculture from Centella asiatica extract have a
significant higher proliferation than the control group and Acalypha indica Linn
extract (p <0.05).
Centella asiatica extract is more useful for increasing cell proliferation rate
and relative cell viability compared to Acalypha indica extracts in PBMC culture
to obtain mesenchymal stem cells that will be used for cell]
 File Digital: 1
Shelf
 T 30202-Pemanfaatan ekstrak-full text.pdf ::
 Metadata
No. Panggil : T30202
Pengarang :
Pengarang/kontributor lain :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2011
Program Studi :
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume
Deskripsi Fisik : xiii, 51 pages : illustration ; 28 cm+ appendix
Catatan Bibliografi : pages 52-56
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
  • Ketersediaan
  • Ulasan
  • Sampul
No. Panggil No. Barkod Ketersediaan
T30202 T30202 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20295114